Culture dependent and independent analysis and appraisal of early stage biofilm-forming bacterial community composition in the Southern coastal seawater of India

Rajeev, Meora; Sushmitha, T.J.; Toleti, Subba Rao; Pandian, Shunmugiah Karutha

doi:10.1016/j.scitotenv.2019.02.171

Cited by 37 publications

(14 citation statements)

References 77 publications

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“…Contrariwise, Alphaproteobacteria were not present in the initial wastewater but had a strong presence in the final cultures and control sample afterwards bilge wastewater was exposed to aerobic batch conditions. Alphaproteobacteria are able to degrade hydrocarbons under aerobic conditions (Cappello et al 2016;Chen et al 2019) and they are commonly found in seawater (Rajeev et al 2019). Similar dynamics are described by Procópio (2020), whereas Alphaproteobacteria is the dominant class in biofilms exposed to oil and chemical surfactants in a marine environment, with Gammaproteobacteria being…”

Section: Aerobic Microbial Profilementioning

confidence: 99%

Comparison of different cultures and culturing conditions for the biological deterioration of organic load from real saline bilge wastewater: microbial diversity insights and ecotoxicity assessment

Mazioti

Vasquez

Vyrides

2021

Environ Sci Pollut Res

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Bilge wastewater is a high strength, typically saline wastewater, originating from operation of ships. In this study, the treatment of real bilge wastewater was tested using pure isolated aerobic strains and mixed cultures (aerobic and anaerobic). The Chemical Oxygen Demand (COD) and ecotoxicity decrease were monitored over time, while the microbial dynamics alterations in mixed cultures were also recorded. The isolated strains Pseudodonghicola xiamenensis, Halomonas alkaliphila and Vibrio antiquaries were shown to significantly biodegrade bilge wastewater. Reasonable COD removal rates were achieved by aerobic mixed cultures (59%, 9 days), while anaerobic mixed cultures showed lower performance (34%, 51 days). The genus Pseudodonghicola was identified as dominant under aerobic conditions both in the mixed cultures and in the control sample (raw wastewater), after exposure to bilge wastewater, demonstrating natural proliferation of the genus and potential contribution to COD reduction. Biodegradation rates were higher when initial organic load was high, while the toxicity of raw wastewater partially decreased after treatment.

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Section: Aerobic Microbial Profilementioning

confidence: 99%

Comparison of different cultures and culturing conditions for the biological deterioration of organic load from real saline bilge wastewater: microbial diversity insights and ecotoxicity assessment

Mazioti

Vasquez

Vyrides

2021

Environ Sci Pollut Res

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“…Recently, to better utilise the periphytic biofilms' capacity of biological purification and degradation, various kinds of artificial substrates have been applied to immobilise periphytons, substituting natural substrates (Witt et al, 2011;An et al, 2018). Previous studies demonstrated that periphytic biofilms on artificial substrates were highly heterogeneous and dynamic, and significantly different from those on natural substrates (rock and wood; Miao et al, 2019;Rajeev et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…At present, different artificial substrates have been used for periphytic biofilm immobilisation and water purification ( Matsumoto et al, 2012 ; Rajeev et al, 2019 ). For example, carbon fibres (CFs; An et al, 2018 ; Liu et al, 2018a ), activated CF (ACF; Chen et al, 2013 ), and polyvinyl chloride (PVC) plastic ( Xia et al, 2010 ; Liu et al, 2014 ; von Ammon et al, 2018 ) are widely used as carriers for microorganisms due to the advantages of their physicochemical properties (i.e., strong stability, superior biocompatibility, and large specific surface area).…”

Section: Introductionmentioning

confidence: 99%

Periphytic Biofilm Formation on Natural and Artificial Substrates: Comparison of Microbial Compositions, Interactions, and Functions

et al. 2021

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Periphytic biofilms have been widely used in wastewater purification and water ecological restoration, and artificial substrates have been progressively used for periphyton immobilisation to substitute natural substrates. However, there is insufficient knowledge regarding the interaction network structure and microbial functions in biofilm communities on artificial substrates, which are essential attribute affecting their applications in biofilm immobilisation. This study compared the community structure, co-occurrence network, and metabolic functions of bacterial and microeukaryotic periphytic biofilms during a 35-day indoor cultivation on artificial substrates, such as artificial carbon fibre (ACF) and polyvinyl chloride (PVC), and natural substrates, such as pebble and wood. Results demonstrated that different types of artificial substrates could affect the community composition and functional diversity of bacterial and microeukaryotic biofilms. The bacterial and microeukaryotic community on ACF and PVC showed significantly higher Simpson index compared to those on wood. Bacterial networks on artificial substrates were more complex than those on natural substrates, while the keystone species on natural substrates were more abundant, indicating that the bacterial communities on artificial substrates had stronger stability and resistance to external interference. Furthermore, the functional metabolic profiles predicted showed the abilities of bacterial communities to metabolise nitrogen and carbon sources colonised on artificial substrates were stronger than those on natural substrates. These findings demonstrated that artificial substrates could be special niches for microbial colonisation, possibly altering microbial compositions, interactions, and functions. Therefore, this study provides a powerful theoretical basis for choosing suitable artificial substrates for microbial aggregation and immobilisation technology.

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“…Biofilms enable to biodegrade and remove organic pollutants at different levels, promoting the metabolism, mineralization, and circulation of essential nutrients in aquatic ecosystems. The biofilm is mainly formed by different microbial species [10] embedded in the extracellular polymeric substances (EPS). Biofilms have mechanical stability and can exist on the surface of solid-liquid, solid-gas, liquid-gas [11].…”

Section: Introductionmentioning

confidence: 99%

“…Biofilms have mechanical stability and can exist on the surface of solid-liquid, solid-gas, liquid-gas [11]. Many factors can affect the colonization and community composition of biofilm, including substrate type, hydrodynamic force, nutrient concentration, and temperature [9,10,12]. The biofilm formation process is also influenced by various environmental factors, including conductivity, TOD (total oxygen demand) and salinity [13].…”

Section: Introductionmentioning

confidence: 99%

Effects of Plastic Debris on the Biofilm Bacterial Communities in Lake Water

Shen

Huang

Xie

et al. 2021

Water

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Increasing discharge of plastic debris into aquatic ecosystems and the worsening ecological risks have received growing attention. Once released, plastic debris could serve as a new substrate for microbes in waters. The complex relationship between plastics and biofilms has aroused great interest. To confirm the hypothesis that the presence of plastic in water affects the composition of biofilm in natural state, in situ biofilm culture experiments were conducted in a lake for 40 days. The diversity of biofilm attached on natural (cobble stones (CS) and wood) and plastic substrates (Polyethylene terephthalate (PET) and Polymethyl methacrylate (PMMA)) were compared, and the community structure and composition were also analyzed. Results from high-throughput sequencing of 16S rRNA showed that the diversity and species richness of biofilm bacterial communities on natural substrate (observed species of 1353~1945, Simpson index of 0.977~0.989 and Shannon–Wiener diversity index of 7.42~8.60) were much higher than those on plastic substrates (observed species of 900~1146, Simpson index of 0.914~0.975 and Shannon–Wiener diversity index of 5.47~6.99). The NMDS analyses were used to confirm the taxonomic significance between different samples, and Anosim (p = 0.001, R = 0.892) and Adonis (p = 0.001, R = 808, F = 11.19) demonstrated that this classification was statistically rigorous. Different dominant bacterial communities were found on plastic and natural substrates. Alphaproteobacterial, Betaproteobacteria and Synechococcophycideae dominated on the plastic substrate, while Gammaproteobacteria, Phycisphaerae and Planctomycetia played the main role on the natural substrates. The bacterial community structure of the two substrates also showed significant difference which is consistent with previous studies using other polymer types. Our results shed light on the fact that plastic debris can serve as a new habitat for biofilm colonization, unlike natural substrates, pathogens and plastic-degrading microorganisms selectively attached to plastic substrates, which affected the bacterial community structure and composition in aquatic environment. This study provided a new insight into understanding the potential impacts of plastics serving as a new habitat for microbial communities in freshwater environments. Future research should focus on the potential impacts of plastic-attached biofilms in various aquatic environments and the whole life cycle of plastics (i.e., from plastic fragments to microplastics) and also microbial flock characteristics using microbial plastics in the natural environment should also be addressed.

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Culture dependent and independent analysis and appraisal of early stage biofilm-forming bacterial community composition in the Southern coastal seawater of India

Cited by 37 publications

References 77 publications

Comparison of different cultures and culturing conditions for the biological deterioration of organic load from real saline bilge wastewater: microbial diversity insights and ecotoxicity assessment

Comparison of different cultures and culturing conditions for the biological deterioration of organic load from real saline bilge wastewater: microbial diversity insights and ecotoxicity assessment

Periphytic Biofilm Formation on Natural and Artificial Substrates: Comparison of Microbial Compositions, Interactions, and Functions

Effects of Plastic Debris on the Biofilm Bacterial Communities in Lake Water

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