Culture conditions influence satellite cell activation and survival of single myofibers

Renzini, Alessandra; Benedetti, A.; Bouché, Marina; Silvestroni, L.; Adamo, Sergio; Moresi, Viviana

doi:10.4081/ejtm.2018.7567

Cited by 14 publications

(12 citation statements)

References 29 publications

(28 reference statements)

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“…These tissues can be utilized to measure contractile function (Brooks and Faulkner, 1988) and assess insulin-stimulated glucose uptake (Hansen et al, 1994). To overcome the short-term culture duration of intact muscle explants, single myofibers can be carefully dissected and isolated from the muscle belly and cultured for up to 7 days (Renzini et al, 2018), though most studies utilize 48-96 h time points. The single myofiber model is the gold-standard model to study satellite cell activation in vitro, due to SCs being retained within their niche and the ability to study SC dynamics with multiple modalities (Pasut et al, 2013;Wang Y. X. et al, 2019).…”

Section: Skeletal Muscle Culture Models Skeletal Muscle Explants and mentioning

confidence: 99%

Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease

Khodabukus

2021

Front. Physiol.

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Skeletal muscle possesses remarkable plasticity that permits functional adaptations to a wide range of signals such as motor input, exercise, and disease. Small animal models have been pivotal in elucidating the molecular mechanisms regulating skeletal muscle adaptation and plasticity. However, these small animal models fail to accurately model human muscle disease resulting in poor clinical success of therapies. Here, we review the potential of in vitro three-dimensional tissue-engineered skeletal muscle models to study muscle function, plasticity, and disease. First, we discuss the generation and function of in vitro skeletal muscle models. We then discuss the genetic, neural, and hormonal factors regulating skeletal muscle fiber-type in vivo and the ability of current in vitro models to study muscle fiber-type regulation. We also evaluate the potential of these systems to be utilized in a patient-specific manner to accurately model and gain novel insights into diseases such as Duchenne muscular dystrophy (DMD) and volumetric muscle loss. We conclude with a discussion on future developments required for tissue-engineered skeletal muscle models to become more mature, biomimetic, and widely utilized for studying muscle physiology, disease, and clinical use.

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Section: Skeletal Muscle Culture Models Skeletal Muscle Explants and mentioning

confidence: 99%

Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease

Khodabukus

2021

Front. Physiol.

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“…Single myofibers were isolated from EDL muscles of 4-6-week-old mice, as described [31][32][33]. Briefly, EDL muscles were dissected from tendon to tendon and incubated in DMEM (Sigma-Aldrich St. Louis, MS, USA) containing 0.2% collagenase I (Sigma-Aldrich) for 45 min.…”

Section: Single Myofiber Isolation and Culturementioning

confidence: 99%

Targeting PKCθ Promotes Satellite Cell Self-Renewal

Benedetti

Fiore

Madaro

et al. 2020

IJMS

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Skeletal muscle regeneration following injury depends on the ability of satellite cells (SCs) to proliferate, self-renew, and eventually differentiate. The factors that regulate the process of self-renewal are poorly understood. In this study we examined the role of PKCθ in SC self-renewal and differentiation. We show that PKCθ is expressed in SCs, and its active form is localized to the chromosomes, centrosomes, and midbody during mitosis. Lack of PKCθ promotes SC symmetric self-renewal division by regulating Pard3 polarity protein localization, without affecting the overall proliferation rate. Genetic ablation of PKCθ or its pharmacological inhibition in vivo did not affect SC number in healthy muscle. By contrast, after induction of muscle injury, lack or inhibition of PKCθ resulted in a significant expansion of the quiescent SC pool. Finally, we show that lack of PKCθ does not alter the inflammatory milieu after acute injury in muscle, suggesting that the enhanced self-renewal ability of SCs in PKCθ-/- mice is not due to an alteration in the inflammatory milieu. Together, these results suggest that PKCθ plays an important role in SC self-renewal by stimulating their expansion through symmetric division, and it may represent a promising target to manipulate satellite cell self-renewal in pathological conditions.

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“…Sodium benzoate is a common preservative added to processed meat products (Hoang and Vu, 2016). Furthermore, antibiotics are often added to the growth media of cells in long-term culture to prevent any infection from bacteria (Burton et al, 2000, Renzini et al, 2018, Stephens et al, 2018. Patents for industrial production of cell-based meat state that the process will be achieved without antibiotics (or hormones) (Van Eelen, 2007).…”

Section: Growth Media For Proliferation Merging and Maturationmentioning

confidence: 99%

Review: Analysis of the process and drivers for cellular meat production

Warner

2019

Animal

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Cell-based meat, also called ‘clean’, lab, synthetic or in vitro meat, has attracted much media interest recently. Consumer demand for cellular meat production derives principally from concerns over environment and animal welfare, while secondary considerations include consumer and public health aspects of animal production, and food security. The present limitations to cellular meat production include the identification of immortal cell lines, availability of cost-effective, bovine-serum-free growth medium for cell proliferation and maturation, scaffold materials for cell growth, scaling up to an industrial level, regulatory and labelling issues and at what stage mixing of myo-, adipo- and even fibrocytes can potentially occur. Consumer perceptions that cell-based meat production will result in improvements to animal welfare and the environment have been challenged, with the outcome needing to wait until the processes used in cell-based meat are close to a commercial reality. Challenges for cell-based meat products include the simulation of nutritional attributes, texture, flavour and mouthfeel of animal-derived meat products. There is some question over whether consumers will accept the technology, but likely there will be acceptance of cell-based meat products, in particular market segments. Currently, the cost of growth media, industry scale-up of specific components of the cell culture process, intellectual property sharing issues and regulatory hurdles mean that it will likely require an extended period for cellular meat to be consistently available in high-end restaurants and even longer to be available for the mass market. The progress in plant-based meat analogues is already well achieved, with products such as the ImpossibleTM Burger and other products already available. These developments may make the development of cellular meat products obsolete. But the challenges remain of mimicking not only the nutritional attributes, flavour, shape and structure of real meat, but also the changes in regulation and labelling.

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Culture conditions influence satellite cell activation and survival of single myofibers

Cited by 14 publications

References 29 publications

Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease

Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease

Targeting PKCθ Promotes Satellite Cell Self-Renewal

Review: Analysis of the process and drivers for cellular meat production

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