Culture conditions influence conidial production by the barley pathogen Ramularia collo-cygni

Sghyer, Hind; Heß, Michael

doi:10.1007/s41348-019-00226-8

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…6,[10][11][12] Environmental conditions, including light intensity, leaf surface wetness, temperature, and water availability, may affect RLS development. 2,[13][14][15] Yield losses of up to 70% have been reported but are more commonly between 5% to 25%, with reductions in grain size and quality, 2, [16][17][18] [see Greif (2002) as cited in Sghyer and Hess 19 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of Ramularia collo‐cygni from barley in Australia using triplex quantitative and droplet digital PCR

Knight

Moslemi

Begum

et al. 2021

Pest Management Science

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BACKGROUND: Ramularia leaf spot (RLS), caused by Ramularia collo-cygni, is an emerging threat to barley (Hordeum vulgare L.) production. RLS has been reported in Australia, however only minimal information is available regarding its detection and distribution. Due to initial asymptomatic growth in planta, slow growth in vitro and symptomatic similarities to net blotch and physiological leaf spots, detection of this pathogen can be challenging. Quantitative polymerase chain reaction (PCR)-based methods for R. collo-cygni-specific identification and detection have been described, however these assays have been demonstrated to lack specificity. False-positive detections may have serious implications, thus we aimed to design a robust R. collocygni-specific PCR method.RESULTS: Using the phylogenetically informative RNA polymerase II second largest subunit (rpb2) and translation elongation factor 1-alpha (tef1-⊍) genes, along with the tef1-⊍ gene of H. vulgare, a triplex assay was developed for both quantitative and droplet digital PCR. The triplex assay detected R. collo-cygni DNA in barley leaves from New South Wales, South Australia, Tasmania, Victoria and Western Australia. No R. collo-cygni DNA was detected in barley seed grown in Western Australia.CONCLUSION: The presence of R. collo-cygni DNA has been confirmed in Australian barley crops, suggesting a distribution ranging across the southern barley growing regions of Australia. The R. collo-cygni-specific assay will be a valuable tool to assist with monitoring the distribution and impact of R. collo-cygni in Australia and other regions.

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Section: Introductionmentioning

confidence: 99%

Detection of Ramularia collo‐cygni from barley in Australia using triplex quantitative and droplet digital PCR

Knight

Moslemi

Begum

et al. 2021

Pest Management Science

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“…Despite the significance of these diseases in causing economic losses, our understanding of the genetic variability among different Ramularia isolates and the underlying factors contributing to this variability remains limited. One potential source of variability among Ramularia isolates is their ability to utilize different carbon sources [2]. Macronutrients, such as carbohydrates, proteins, lipids, and nucleic acids, are composed of essential elements including carbon, hydrogen, nitrogen, sulphur, and phosphorus.…”

Section: Introductionmentioning

confidence: 99%

Carbon Source Utilisation as a Basis of Variability among Different Ramularia Isolates Collected from Cotton Belt of Odisha, India

Boblina

Senapati

Panda

et al. 2022

IJECC

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The impact of various carbon sources on the growth and sporulation of six different Ramularia isolates was investigated by testing eight different sources of carbon in liquid Richard's medium. The mycelial growth was assessed by determining the dry biomass weight, and there were three replicate flasks for each combination of isolates and carbon source. The study showed that the extent of utilization of different carbon sources varied among the isolates, and the highest mean dry biomass weight was recorded by RGKN1 followed by RGGNPR1. Maltose and sucrose were found to be the best source of carbon supporting highest mycelial growth and sporulation. Starch and lactose supported the least mycelial growth and sporulation. The results clearly established the essentiality of carbon source for metabolic activities of the fungus. The study proved the existence of cultural variability among the Ramularia isolates basing on their carbon utilisation.

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“…Environmental conditions, including light intensity, leaf surface wetness, temperature and water availability, may affect RLS development (Havis et al 2015b;Hoheneder et al 2021b;Makepeace et al 2008;McGrann and Brown 2018). Yield losses of up to 70% have been reported but are more commonly between 5 to 25%, with reductions in grain size and quality (Harvey 2002;Havis et al 2015b;Pereyra et al 2014;Pinnschmidt and Jørgensen 2009; Greif 2002 as cited in Sghyer and Hess 2019).…”

Section: Introductionmentioning

confidence: 99%

Detection ofRamularia collo-cygnifrom barley (Hordeum vulgare) in Australia using triplex quantitative and digital PCR

Knight

Moslemi

Begum

et al. 2021

Preprint

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Ramularia leaf spot (RLS), caused by Ramularia collo-cygni, is an emerging threat to barley (Hordeum vulgare) production. RLS has been reported in Australia; however only minimal information is available regarding its detection and distribution. Due to initial asymptomatic growth in planta, slow growth in vitro and symptomatic similarities to net blotch and physiological leaf spots, detection of this pathogen can be challenging. Quantitative PCR-based methods for R. collo-cygni-specific identification and detection have been described, however these assays (based upon the internal transcribed spacer [ITS] region) have been demonstrated to lack specificity. False-positive detections may have serious implications, thus we aimed to design a robust R. collo-cygni-specific PCR method. Using the phylogenetically informative RNA polymerase II second largest subunit (rpb2) and translation elongation factor 1-α (tef1-α) genes, along with the tef1-α gene of H. vulgare, a triplex assay was developed for both quantitative and digital PCR. The triplex assay was used to assess DNA of barley leaves from New South Wales, South Australia, Tasmania, Victoria and Western Australia, along with DNA of seeds from Western Australia. Detection of R. collo-cygni DNA was confirmed for leaf samples from New South Wales, South Australia, Tasmania, Victoria and Western Australia, indicating a distribution ranging across the southern barley growing regions of Australia. No R. collo-cygni DNA was detected in seed from Western Australia. The R. collo-cygni-specific assay will be a valuable tool to assist with monitoring the distribution of R. collo-cygni in Australia and other regions.

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Culture conditions influence conidial production by the barley pathogen Ramularia collo-cygni

Cited by 6 publications

References 25 publications

Detection of Ramularia collo‐cygni from barley in Australia using triplex quantitative and droplet digital PCR

Detection of Ramularia collo‐cygni from barley in Australia using triplex quantitative and droplet digital PCR

Carbon Source Utilisation as a Basis of Variability among Different Ramularia Isolates Collected from Cotton Belt of Odisha, India

Detection ofRamularia collo-cygnifrom barley (Hordeum vulgare) in Australia using triplex quantitative and digital PCR

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