Culture conditions for mycelial growth of Poria cocos

Jo, Woo-Sik; Park, Ju-Ri; Oh, So-Ra; Kang, Min-Gu; Kim, Woo-Hyun; Park, Seung-Chun

doi:10.14480/jm.2016.14.1.6

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Journal of Mushroom

2016

DOI: 10.14480/jm.2016.14.1.6

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Culture conditions for mycelial growth of Poria cocos

Woo-Sik Jo¹,

Ju-Ri Park²,

So-Ra Oh³

et al.

Abstract: This study was carried out to determine the basic mycelial culture conditions for Poria cocos growth. According to colony diameter and mycelial density, suitable media for mycelial growth were Malt yeast extract, Potato dextrose agar, Yeast extract agar, and Yeast malt agar. The optimum temperature for mycelial growth was between 25 and 35 o C, and the optimum pH value was between 4 and 7. Carbon and nitrogen sources were fructose and yeast extract. The optimum C/N ratio was about 10 to 1 with 2% glucose. Othe… Show more

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2024

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Cited by 3 publications

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Interactions between two strains of lactic acid bacteria and Laetiporus sulphureus strain FH24 and FH319, and Wolfiporia cocos strain FH9 mycelium

Todorov,

Orban,

Hammer

et al. 2024

LWT

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Interactions between two strains of lactic acid bacteria and Laetiporus sulphureus strain FH24 and FH319, and Wolfiporia cocos strain FH9 mycelium

Todorov,

Orban,

Hammer

et al. 2024

LWT

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An omics-based characterization of Wolfiporia cocos reveals three CYP450 members involved in the biosynthetic pathway of pachymic acid

Liu,

Jing,

et al. 2024

Commun Biol

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Wolfiporia cocos is a medicinal mushroom used in China. It biosynthesizes pachymic acid (PA), a main therapeutic triterpene associated with therapies. Nowadays, the unknown PA biosynthesis leads to difficulties in increasing its content in W. cocos. Herein, we report sequencing, assembling, and characterization of the genome and several transcriptomes of W. cocos. Sequence mining determined candidate genes that encode lanosterol synthase, sterol O-acyltransferase, and sterol C-24 methyltransferase likely involved in the steps from lanosterol to PA. Gene cluster analysis identified four CYP450 cDNAs likely involved in the biosynthesis of PA, namely WcCYP64-1, WcCYP64-2, WcCYP52, and WcCYP_FUM15, which were subjected to both overexpression and silencing in mycelia. The overexpression of each of WcCYP64-1, WcCYP52 and WcCYP_FUM15 increased the content of PA, 16α-hydroxytrametenolic acid, eburicoic acid, and tumulosic acid, while the silencing of each gene either significantly or slightly decreased the contents of these four compounds, indicating their involvement in the PA biosynthesis. In addition, different temperatures affected the expression of these genes and the formation of PA. By contrast, the overexpression and silencing of WcCYP64-2 did not alter the formation of these compounds. Taken together, these findings determine more potential steps in the biosynthetic pathway of PA for metabolic engineering.

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Optimizing liquid fermentation for Wolfiporia cocos: gene expression and biosynthesis of pachymic acid and mycelial biomass

Noushahi,

Khan,

Khan

et al. 2024

Letters in Applied Microbiology

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Wolfiporia cocos, a versatile fungus acclaimed for its nutritional and therapeutic benefits in Traditional Chinese Medicine, holds immense potential for pharmaceutical and industrial applications. In this study, we aimed to optimize liquid fermentation techniques and culture medium composition to maximize mycelial biomass (MB) yield, pachymic acid (PA) concentration, and overall PA production. Additionally, we investigated the molecular basis of our findings by quantifying the expression levels of genes associated with PA and MB biosynthesis using quantitative real-time polymerase chain reaction. Under the optimized fermentation conditions, significant results were achieved, with maximum MB reaching 6.68 g l−1, PA content peaking at 1.25 mg g−1, and a total PA yield of 4.76 g l−1. Notably, among the four examined genes, squalene monooxygenase, exhibited enhanced expression at 0.06 ratio under the optimized conditions. Furthermore, within the realm of carbohydrate-active enzymes, the glycoside hydrolases 16 family displayed elevated expression levels at 21 ratios, particularly during MB production. This study enhances understanding of genetic mechanism governing MB and PA production in W. cocos, highlighting the roles of squalene monooxygenase and glycoside hydrolases 16 carbohydrate-active enzymes.

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Culture conditions for mycelial growth of Poria cocos

Cited by 3 publications

References 2 publications

Interactions between two strains of lactic acid bacteria and Laetiporus sulphureus strain FH24 and FH319, and Wolfiporia cocos strain FH9 mycelium

Interactions between two strains of lactic acid bacteria and Laetiporus sulphureus strain FH24 and FH319, and Wolfiporia cocos strain FH9 mycelium

An omics-based characterization of Wolfiporia cocos reveals three CYP450 members involved in the biosynthetic pathway of pachymic acid

Optimizing liquid fermentation for Wolfiporia cocos: gene expression and biosynthesis of pachymic acid and mycelial biomass

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