CULTURE CHARACTERISTICS AND ENZYMATIC ACTIVITY OF
 
 SARCODONTIA CROCEA
 
 (
 
 BASIDIOMYCOTA
 
 ) STRAINS COLLECTED FROM THE CENTRAL RUSSIAN UPLAND

Шахова, Н. В.; Volobuev, Sergey

doi:10.31857/s0026364820060100

Cited by 6 publications

(5 citation statements)

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“…Besides the specialisation in development on certain host species (S. crocea prefers the wood of Malus spp. ), this group of fungi is characterized by being associated with living plants, remaining vitality on dead plant remnants only for a short time Shakhova, Volobuev, 2020a). Apparently, chips or nutrient media based on water extracts from the chips of seed fruit trees were treated by S. crocea strains as an organic matter of a dead plant, as evidenced by low growth rate, depressed mycelial mat and partial loss of the activity of lignolytic complex enzymes.…”

Section: Resultsmentioning

confidence: 99%

“…Fungal strains were isolated ex situ both from basidiocarps and basidiospores on the territory of Belgorod, Oryol and Rostov Regions in 2006-2019 using the traditional methods of solid phase cultivation (Shakhova, Volobuev, 2020b). The identification of strains was performed using the ITS1-5.8S-ITS2 nrDNA analysis (Shakhova, Volobuev, 2020a) and based on the cultural and morphological parameters described by J. A. .…”

Section: Methodsmentioning

confidence: 99%

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A Comparative Study on Growth and Lignocellulolytic Activity of Nine Sarcodontia Crocea Strains in Four Different Media

Volobuyev¹,

Шахова²

2020

DPW

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The results of a study of growth characteristics, macromorphological features and biosynthetic potential of nine dikaryotic strains of Sarcodontia crocea (Polyporales, Basidiomycota) maintained in the Komarov Botanical Institute Basidiomycetes Culture Collection (LE-BIN) are presented. The strains studied were extracted from the basidiocarps collected on Malus domestica in the Belgorod, Oryol and Rostov Oblasts, as well as by seeding of basidiospores. The cultural and morphological characteristics and enzymatic activity of S. crocea were tested on both standard nutrient media (malt-extract agar – MEA, glucose-peptone agar – GPA) and modified semi-synthetic agarized media. Original compositions has been developed and for the first time nutrient media pre-pared using water-based wood extracts from Malus domestica (Malus-M) and Pyrus communis (Pyrus-M) have been approved. It was found that a significant reduction in growth rates was observed during the cultivation of S. crocea on agarized nutrient media of GPA, Malus-M and Pyrus-M. The studied strains on Malus-M and Pyrus-M exhibited high colony variability, sparse micelian mat, and loss of zonality and air mycelium intensity compared to MEA. It was shown that the composition of the nutrient medium strongly determined the ability of S. crocea strains to produce lignocelluolytic complex enzymes. The cellulolytic activity was noted for strains on all media studied, but no reliable differences were found in the cultivation of strains on sugar-rich MEA and three other semi-synthetic media. Only two strains (LE-BIN 2138 and 4355) were identified as having high cellulolytic activ-ity when grown on MEA. The absence of lignolytic complex enzyme activity was demonstrated when the strains were cultivated on new modified semi-synthetic agarized media of Malus-M and Pyrus-M.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A Comparative Study on Growth and Lignocellulolytic Activity of Nine Sarcodontia Crocea Strains in Four Different Media

Volobuyev¹,

Шахова²

2020

DPW

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“…Kotl. ), and its biosynthetic potential [63,64]; and on a stenotrophic basidiomycete found in the Republic of Dagestan, Fomitiporia hippophaeicola (H. Jahn) Fiasson & Niemelä, which is the causative agent of sea buckthorn (Hippophaë rhamnoides) wood rot [65]; and on a xylothrophic fungus, Fistulina hepatica LE-BIN 3801 [66].…”

Section: Cultural Characteristicsmentioning

confidence: 99%

“…Donk (32 strains). Two Pleurotus species are widely distributed, abundantly fruiting in nature, and easy to culture, while such a number of strains of a rather rare species S. setosa can be explained by a special interest in its investigation [63,64]. More than 20 strains of the following species are preserved in the collection: 28 strains of Lentinula edodes (Berk.)…”

Section: Molecular Analysismentioning

confidence: 99%

The Conservation and Study of Macromycetes in the Komarov Botanical Institute Basidiomycetes Culture Collection—Their Taxonomical Diversity and Biotechnological Prospects

Psurtseva,

Kiyashko,

Senik

et al. 2023

JoF

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Culture collections (CCs) play an important role in the ex situ conservation of biological material and maintaining species and strains, which can be used for scientific and practical purposes. The Komarov Botanical Institute Basidiomycetes Culture Collection (LE-BIN) preserves a large number of original dikaryon strains of various taxonomical and ecological groups of fungi from different geographical regions. Started in the late 1950s for the investigation of Basidiomycetes’ biological activity, today, in Russia, it has become a unique specialized macromycetes collection, preserving 3680 strains from 776 species of fungi. The Collection’s development is aimed at ex situ conservation of fungal diversity, with an emphasis on preserving rare and endangered species, ectomycorrhizal fungi, and strains useful for biotechnology and medicine. The main methods applied in the collection for maintaining and working with cultures are described, and the results are presented. Some problems for the isolation and cultivation of species are discussed. The taxonomical structure and variety of the strains in the collection fund are analyzed, and they show that the taxonomical diversity of fungi in the LE-BIN is commensurable with the largest CCs in the world. The achievements from the ex situ conservation of the diversity of macromycetes and the main results from the screening and investigation of the collection’s strains demonstrate that a number of strains can be prospective producers of enzymes (oxidoreductases and proteases), lipids, and biologically active compounds (terpenoids, phthalides, etc.) for biotechnology and medicine.

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“…Occasionally there were observed chlamydospores broadly ellipsoid (width -4.0 (5.0)-5.5 (6.6) µm, length -10.0 (15.0)-17.0 (24.0) µm) or globose chlamydospores (10.0-16.0 µm in diameter). Detailed descriptions of some strains are presented in Shakhova and Volobuev [53]. ECOLOGY: S. crocea is the most abundant pathogen of Malus trees.…”

Section: Boletales Coniophoraceaementioning

confidence: 99%

Synopsis of the macrofungi (Basidiomycota) on wood of fruit trees in the Central Black Earth Region of Russia

Volobuev

Большаков

Шахова

2021

Ûg Ross.: èkol. razvit.

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Aim. To summarise and analyse data on the species diversity, distribution and substrate spectra of wood-inhabiting basidial macromycetes growing on fruit trees in the Belgorod, Voronezh, Kursk, Lipetsk, Oryol and Tambov Regions.Material and Methods. The work involved a critical examination of literature sources, LE, OHHI, and VU herbaria. We undertook field researches in 2019-2020, identified specimens collected based on light microscopy techniques and isolated the basidial fungi in a pure culture.Results. Data on 97 species of basidial macrofungi from 68 genera associated with Malus, Pyrus and Prunus wood in the Central Black Earth Region are summarised. Antrodia serpens and Ceriporia torpida are newly known to Russia. 65 new dikaryot-ic strains for 22 species of Agaricomycetes have been introduced into the LE-BIN. A total of 65 species are associated with Malus, 34 species with Pyrus and 29 species with Prunus. Five species (Lyomyces crustosus, Stereum hirsutum, Trametes hirsuta, T. ochracea and T. versicolor) grow on all three genera of host. These species specialise in fruit trees include Sarcodontia crocea and Phellinus pomaceus.Conclusion. Pathogenic activity has been clearly observed for 32 species of fungi found on trunks and branches of living trees, causing necrosis and trunk rot. Furthermore, it is recommended that regular phytopathological monitoring of orchards should be carried out, taking the group of xylotrophic fungi into account.

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CULTURE CHARACTERISTICS AND ENZYMATIC ACTIVITY OF SARCODONTIA CROCEA ( BASIDIOMYCOTA ) STRAINS COLLECTED FROM THE CENTRAL RUSSIAN UPLAND

Cited by 6 publications

References 0 publications

A Comparative Study on Growth and Lignocellulolytic Activity of Nine Sarcodontia Crocea Strains in Four Different Media

A Comparative Study on Growth and Lignocellulolytic Activity of Nine Sarcodontia Crocea Strains in Four Different Media

The Conservation and Study of Macromycetes in the Komarov Botanical Institute Basidiomycetes Culture Collection—Their Taxonomical Diversity and Biotechnological Prospects

Synopsis of the macrofungi (<i>Basidiomycota</i>) on wood of fruit trees in the Central Black Earth Region of Russia

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