2019
DOI: 10.1371/journal.pone.0212922
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Culture and Real-time Polymerase Chain reaction sensitivity in the diagnosis of invasive meningococcal disease: Does culture miss less severe cases?

Abstract: BackgroundInvasive meningococcal disease (IMD) is a highly lethal disease. Diagnosis is commonly performed by culture or Realtime-PCR (qPCR).AimsOur aim was to evaluate, retrospectively, whether culture positivity correlates with higher bacterial load and fatal outcome. Our secondary aim was to compare culture and qPCR sensitivity.MethodsThe National Register for Molecular Surveillance was used as data source. Cycle threshold (CT), known to be inversely correlated with bacterial load, was used to compare bacte… Show more

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Cited by 25 publications
(18 citation statements)
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References 20 publications
(33 reference statements)
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“…Clinical detection of Neisseria meningitidis in many countries currently uses PCR, ELISA, and bacterial culture,[ 9 ] and PCR is considered the most authoritative standard. However, these technologies have limited applicability in poor and developing countries.…”
Section: Introductionmentioning
confidence: 99%
“…Clinical detection of Neisseria meningitidis in many countries currently uses PCR, ELISA, and bacterial culture,[ 9 ] and PCR is considered the most authoritative standard. However, these technologies have limited applicability in poor and developing countries.…”
Section: Introductionmentioning
confidence: 99%
“…One reason that could explain the increase in IMDs is the peak of cases recorded in Tuscany in 2015 and 2016, especially in adolescents and young adults [ 40 , 43-45 ] and in Liguria in 2016 and 2017 [ 40 , 41 ]. Moreover, there have also been improvements in the laboratory diagnosis confirmation system, both at regional and national level [ 33 ]. For example, in Tuscany during 2015, real-time polymerase chain reaction (PCR), a more sensitive, rapid and accurate laboratory diagnostic test [ 33 ], was made available to all hospitals by a regional law [ 46 ], reducing the underestimation of IMD [ 29 ] and consequently improving the accuracy of the surveillance system and differentiating IBIs [ 29 , 33 , 47 ].…”
Section: Discussionmentioning
confidence: 99%
“…The clinical detection of Neisseria meningitidis in many countries currently uses PCR, ELISA, and bacterial culture [8] , and PCR is considered the most authoritative standard. However, these technologies have limited applicability in poor and developing countries.…”
Section: Introductionmentioning
confidence: 99%