Cultural Isolation and PCR Based Assay for Detection of flaA gene of Campylobacter jejuni from Acute Diarrheic Patients in Tertiary Care Hospital at Dhaka, Bangladesh

Roy, Sushmita; Shamsuzzaman, SM; Mamun, Kazi Zulfiquer; Ahmed, Mejbah Uddin; Halder, Soma

doi:10.9734/bmrj/2015/19818

Cited by 3 publications

(5 citation statements)

References 27 publications

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“…In this study, higher sensitivity rate were observed for cipro oxacin (69%) and nalidixic acid (65.5%) and this nding is in line with similar study done elsewhere in Ethiopia [13] and beyond [55,56]. Highest sensitivity rate of nalidixic acid (100%) were reported in Jimma, Ethiopia [12], Sudan [38] and Zambia [40] compared to the present study.…”

Section: Discussionsupporting

confidence: 91%

Campylobacter Jejuni and Its Antimicrobial Susceptibility Pattern Among Under- Five Children with Gastroenteritis in Northwest Ethiopia

wudie

Melese

Mekonnen

2021

Preprint

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Introduction: Campylobacter jejuni is the leading causes of diarrheal disease worldwide among under five years of age. Epidemiological data about C. jejuni and drug susceptibility profile of isolates among children with symptom of gastroenteritis has a paramount importance for evidence based decision. However, such information is not available in the study area. Hence, this study was carried out fill this information gap.Methods: An institution based cross-sectional study was conducted among under-five children (U5C) visiting four primary health centers’ pediatric clinic due to acute gastroenteritis from February to May, 2019. Socio-demographic data were collected in a face to face interview using structured questionnaire. Stool specimen was collected and transported to the Bahir Dar University, Medical Microbiology Laboratory. The specimens were inoculated on Charcoal Cefoperazone Deoxycholate Agar. Identification and antibacterial susceptibility test was performed using standard bacteriological methods. The collected data were entered to EpiData 3.1 and analyzed using SPSS version 23.Results: Among a total of 196 U5C with acute gastroenteritis, 39 (19.9%) were positive for Campylobacter species. The proportion of C. jejuni was 14.8% (29/196). The multivariate logistic regression analyses revealed that care givers (AOR=3.76, 95%CI: 1.29-10.98), guardians hand washing practice before food preparation (AOR=3.63, 95%CI: 1.15-11.46), and contact with domestic animals (AOR=4.23, 95%CI: 1.46-12.28) demonstrated significant association with C. jejuni infection. Among 29 C. jejuni isolates, 24 (82.8%), 24 (82.8%) and 18 (62.1%) were resistant for tetracycline, cefoxitine and erythromycin, respectively. Conclusions: The proportion of C. jejuni infection was high. Children who had contact with animals showed association with C. jejuni infection. Additionally, majority of C. jejuni showed resistance for tetracycline, cefoxitin, ampicillin and erythromycin.

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Section: Discussionsupporting

confidence: 91%

Campylobacter Jejuni and Its Antimicrobial Susceptibility Pattern Among Under- Five Children with Gastroenteritis in Northwest Ethiopia

wudie

Melese

Mekonnen

2021

Preprint

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“…Several studies in Bangladesh revealed 13.5-17.4% Campylobacter species in diarrhoeal children. 3,11,18,19 Campylobacter jejuni were isolated from diarrhoeic stool samples was 15.4% in Northwest Ethiopia, 20 12.7% in South Ethiopia, 21 18% in Pakistan, 9 which were in accordance with the present study. But higher prevalence of Campylobacter species were detected in diarrhoeic children 21%.…”

Section: Discussionsupporting

confidence: 91%

“…In this study, among 24 isolated Campylobacter species 91.7% of cases were Campylobacter jejuni and 8.3% of cases were Campylobacter coli. Roy et al 11 reported 88.89% of cases were Campylobacter jejuni and 11.11% of cases were Campylobacter coli among isolated Campylobacter spp. in Bangladesh.…”

Section: E DI Erence In Frequency Ofmentioning

confidence: 98%

“…10 In Bangladesh, isolation rate of Campylobacter jejuni causing acute childhood diarrhoea is 17.4%, 3 and 12%. 11 In general, the transmission of Campylobacter occurs to humans by eating undercooked or contaminated poultry, unpasteurised dairy products and contaminated water or coming with the contact faeces of a dog or cat. But person-to-person spread of Campylobacter is not frequent.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of Campylobacter Species in the Stool of Under Five Children With Acute Diarrhea in a Tertiary Care Hospital of Bangladesh

et al. 2021

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Campylobacter species is the main etiology of gastroenteritis due to bacteria. To determine prevalence of Campylobacter species in stool of children less than five years of age with acute diarrhoea, this observational study was conducted in the Department of Microbiology, Sylhet M A G Osmani Medical College, Sylhet from January to December, 2017. Stool samples were collected from 162 under-five children with acute diarrhoea admitted in the Department of Paediatrics. Isolation of Campylobacter species were done by stool culture. About two third of the children were male (65.4%) and more than one fourth of the affected children (26.65%) were in age group 6-12 months. Campylobacter species was isolated in 24 (15%) sample and among them, Campylobacter jejuni were 22 (91.7%) and Campylobacter coli were 2 (8.3%). Isolation rate of Campylobacter species did not differ significantly between age group of 6-12 months and above 12 months (p=0.211) of age; male and female children (p=0.288); among socioeconomic status (p=0.673) and between residential status (p=0.108). Isolation rate of Campylobacter species are frequent among under five children with acute diarrhea and most of the children came from low socioeconomic background and were male. However, a large multicenter study needs to be conducted to generate more evidence regarding the issue. Bangladesh Med J. 2020 Sept; 49(3) : 11-16

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“…In Dhaka, Roy reported 13.5% Campylobacter found in acute diarrhoeal patients. 17 So the rate of isolation varies from place to place where contaminated water and food of animal origin, especially improperly processed chicken acts as vehicle for transmission of campylobacteriosis of human. 18 In this study Shigella spp.…”

Section: Discussionmentioning

confidence: 99%

Causative agents for diarrhoea in under 5 children in a tertiary care hospital

Pervin

Jhora²,

Paul³

et al. 2019

Bangladesh med. j. Khulna

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Background: nfectious diarrhoea is a major public health problem in developing countries like Bangladesh. Bacteria and parasites are important etiological agents for diarrhoea in infants and children. Objectives: To isolate and identify the causative organisms of diarrhoea from diarrhoeal stools in under 5 children. Methodology: This was an observational cross sectional study carried out at Microbiology Department of Sir Salimullah Medical College, Dhaka. A total of 217 diarrhoeal patients upto 5 years age, attending Pediatric unit of Sir Salimullah Medical College and Mitford Hospital, Dhaka and Dhaka Shishu Hospital, Dhaka during the period of July 2015 to June 2016, were enrolled in the study. For isolation of enteric bacteria, diarrhoeal stools were inoculated on Mackonkey agar media and for Campylobacter spp. diarrhoeal stools were inoculated on selective Modified Campy-BAP media and nonselective Blood agar media with membrane filter. Positive culture was confirmed by Gram stain morphology and standard biochemical tests. Oocysts of Cryptosporidium were identified by Modified Ziehl Neelsen staining and other parasites were identified by wet mount preparation. Results: Out of 217 diarrhoeal stools bacteria were isolated from 97 (44.7%) patients. Among 97 cases, Escherichia coli were detected in 51(52.58%) patients; Campylobacter spp. were detected in 23(23.71%) patients; Shigella spp. were detected in 15(15.46%) patients and Salmonella spp. were detected in 8(8.25%) patients. Microscopic finding of diarrhoeal stools were detected by pus cells with or without RBCs in 90(70.31%) samples. Cysts of Giardia intestinalis were found in 9(4.14%) samples, cysts of Entamoeba histolytica in 2(0.9%) samples, eggs of Trituris trichiura in 2(0.9%) samples and oocysts of Cryptosporidium were found in.5(2.30%) samples. Conclusion: Escherichia coli was the most common bacteria and Giardia intestinalis was predominant parasite causing diarrhoea in under 5 children. Bang Med J (Khulna) 2018; 51 : 25-28

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Cultural Isolation and PCR Based Assay for Detection of flaA gene of Campylobacter jejuni from Acute Diarrheic Patients in Tertiary Care Hospital at Dhaka, Bangladesh

Cited by 3 publications

References 27 publications

Campylobacter Jejuni and Its Antimicrobial Susceptibility Pattern Among Under- Five Children with Gastroenteritis in Northwest Ethiopia

Campylobacter Jejuni and Its Antimicrobial Susceptibility Pattern Among Under- Five Children with Gastroenteritis in Northwest Ethiopia

Isolation of Campylobacter Species in the Stool of Under Five Children With Acute Diarrhea in a Tertiary Care Hospital of Bangladesh

Causative agents for diarrhoea in under 5 children in a tertiary care hospital

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