Cultural and pathogenic variation in potato isolates of Rhizoctonia solani in Northern Ireland

Chand, Tara; Logan, C.

doi:10.1016/s0007-1536(83)80129-6

Cited by 49 publications

(30 citation statements)

References 5 publications

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“…The results are consistent with what was obtained in Britain by Woodhall et al (2007) where he focused most of the isolates (92.6%) to the group AG3 PT. Similar results were reported in France by Campion et al (2003), the same results happened in Northern Ireland (Chand and Logan, 1983). In the State of Michigan America, three anastomosis groups were found; these are: AG2-2, AG3 and AG4, AG3 and considered the pathogenic isolates of the potato (Wharton et al, 2007).…”

Section: Discussionsupporting

confidence: 61%

Pathogenicity Test and Anastomosis Group of Rhizoctonia solani the Causal Organism of Stem Canker and Black Scurf Disease of Potato in Egypt

Abdel-Sattar

El-Marzouky

Ibrahim

2017

Journal of Applied Plant Protection

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Stem canker and black scurf caused by Rhizoctonia solani Kuhn is considered the most destructive disease and reduce productivity of potato. Pathogenicity test of 12 isolates of R. solani obtained from two governorates, ElSharkiya (New El-Salhia region) and Ismailia (El-Shabab region) revealed that all isolates were pathogenic, however isolate No.RS7 was the most virulent isolate in the development of the stem canker and black scurf disease. Molecular technique using PCR applied to determine anastomosis group of isolate No. RS7 showed that this isolate belong to AG3.

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Section: Discussionsupporting

confidence: 61%

Pathogenicity Test and Anastomosis Group of Rhizoctonia solani the Causal Organism of Stem Canker and Black Scurf Disease of Potato in Egypt

Abdel-Sattar

El-Marzouky

Ibrahim

2017

Journal of Applied Plant Protection

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“…Other studies have indicated that AG2-1 isolates from potato are generally not very virulent on potato plants (Carling andLeiner 1990a, Woodhall et al 2007), which was found also in this study. The black scurf symptoms caused by one Finnish AG2-1 isolate in one experiment were very mild, consistent with previous reports in which AG2-1 has been occasionally recovered from black scurf on tubers grown in the fi eld in Alaska (Carling and), Northern Ireland (Chand and Logan 1983) and the United Kingdom (Woodhall et al 2007).…”

Section: Resultssupporting

confidence: 90%

“…The remaining isolates were of AG2-1 or AG5 (Campion et al 2003, Woodhall et al 2007. Similar results indicating predominance of AG3 and occasional occurrence of other AGs have been reported from many other countries including Ireland (Chand and Logan 1983), Alaska ), Canada (Bains and Bisth 1995), USA (Bandy et al 1984(Bandy et al , 1988, Peru (Anquiz and Martin 1989), South Africa (Truter and Wehner 2004), China (Chang and Tu 1980), Japan (Abe and Tsuboki 1978), and Australia (Balali et al 1995).…”

Section: Introductionsupporting

confidence: 78%

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Formation of canker lesions on stems and black scurf on tubers in experimentally inoculated potato plants by isolates of AG2-1, AG3 and AG5 of Rhizoctonia solani: a pilot study and literature review

Lehtonen¹,

Wilson²,

Ahvenniemi³

et al. 2008

AFSci

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Development of black scurf on potato tubers (cv. Nicola) was compared in plants inoculated with isolates of Rhizoctonia solani of three anastomosis groups (AG2-1, AG3 and AG5) which occur in potato crops in Finland. All isolates induced stem canker lesions but only isolates of AG3 formed efficiently black scurf on progeny tubers. Among the AG2-1 and AG5 isolates tested, only one AG2-1 isolate formed a few sclerotia on 13.5 % of the progeny tubers in one experiment. The data indicate that isolates of AG3 differ from those of AG2-1 and AG5 in having a higher ability to form sclerotia on tubers. Therefore, while AG2-1 and AG5 isolates have a broader host range, AG3 is more efficient in producing black scurf, which provides this anastomosis group with more efficient means of dissemination on seed potatoes. These differences probably explain the predominance of AG3 (98.9 % of isolates) in potato crops in Finland and other northern potato production areas.

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“…The R. solani strain Ci96, provided by Neiker Institute (Vitoria, Spain) was grown on potato dextrose agar (PDA) medium [20% (w/v) potato, 1.5% (w/v) glucose and 1.5% (w/v) agar] at 25°C with light for 5 days, and subcultured as needed. The inoculation was done as described by Chand & Logan (1983). After 4 months, the crop was harvested and the tuber symptoms were assessed in five tubers per plant.…”

Section: Resistance Evaluation Of the Transgenic Plants In Greenhousementioning

confidence: 99%

Enhanced resistance to Rhizoctonia solani by combined expression of chitinase and Ribosome Inactivating Protein in transgenic potatoes (Solanum tuberosum L.)

Mahmoud¹,

Chikh-Rouhou²,

Boughalleb³

et al. 2012

Span. j. agric. res.

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Potato (Solanum tuberosum L.) is susceptible to many fungal pathogens including Rhizoctonia solani. In the present study, the potato cultivar Desirée was transformed via Agrobacterium tumefaciens strain GV3101 containing the binary plasmid pGJ132 harboring both the chitinase (chiA) and rip30 genes. The potato leaf disc was used as an explant for transformation. PCR, Southern blot and Western blot were used for characterization of the transgenic plants. In this study it was shown that not all the plants developed in selective medium were positive for the corresponding gene using the PCR technique. Southern blot analysis confirmed that transgenic plants integrated 2-3 copies of chiA and rip30 genes respectively into their genome. The expression of the CHIA and RIP30 proteins was confirmed in the leaf extracts of the transgenic clones by Western blot analysis. Transgenic potato plants expressing rip30 and chiA genes showed enhanced resistance to R. solani in a greenhouse assay.Additional key words: fungal resistance; genes; proteins; southern blot; western blot. ResumenResistencia aumentada a Rhizoctonia solani por la expresión combinada de quitinasa y proteínas inactivantes de los ribosomas en patatas transgénicas La patata (Solanum tuberosum L.) es susceptible a muchos hongos fitopatógenos, incluyendo Rhizoctonia solani. En el presente estudio, se transformó el cultivar de patata 'Desirée' mediante Agrobacterium tumefaciens, cepa GV3101, que contiene el plásmido binario pGJ132 que alberga los genes quitinasa (chiA) y rip30. Se utilizaron discos de hojas como explante para la transformación de plantas. Se utilizaron las técnicas de PCR, Southern y Western blot para la caracterización de las plantas transgénicas. En este estudio se demostró, mediante PCR, que no todas las plantas que se desarrollaron en medio selectivo fueron positivas para el gen correspondiente. El análisis de Southern blot confirmó que las plantas transgénicas integraron en su genoma 2-3 copias de los genes chiA y rip30. Se llevó a cabo un ensayo de invernadero para evaluar la resistencia a R. solani de los clones transgénicos que expresan los transgenes. Las plantas transgénicas que expresan los genes rip30 y chiA mostraron una resistencia completa a R. solani.

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Cultural and pathogenic variation in potato isolates of Rhizoctonia solani in Northern Ireland

Cited by 49 publications

References 5 publications

Pathogenicity Test and Anastomosis Group of Rhizoctonia solani the Causal Organism of Stem Canker and Black Scurf Disease of Potato in Egypt

Pathogenicity Test and Anastomosis Group of Rhizoctonia solani the Causal Organism of Stem Canker and Black Scurf Disease of Potato in Egypt

Formation of canker lesions on stems and black scurf on tubers in experimentally inoculated potato plants by isolates of AG2-1, AG3 and AG5 of Rhizoctonia solani: a pilot study and literature review

Enhanced resistance to Rhizoctonia solani by combined expression of chitinase and Ribosome Inactivating Protein in transgenic potatoes (Solanum tuberosum L.)

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