Cultural and Antigenic Properties of Newly Established Cell Strains Derived From Adenocarcinomas of the Human Colon and Rectum

Tompkins, W. A. F.; Watrach, A. M.; Schmale, John D.; Schultz, Richard M.; Harris, Jennifer A.

doi:10.1093/jnci/52.4.1101

Cited by 251 publications

(93 citation statements)

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“…The Mebus strain of BCV was grown on the human rectal carcinoma cell line, HRT 18 (Tompkins et al, 1974), as previously described (King & Brian, 1982;Hogue et al, 1984). Cells were grown as monolayers in Dulbecco's modified Eagle's medium containing 50 p.g/ml gentamicin (Sigma) and 10% foetal bovine serum (Sterile Systems).…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Processing of the Bovine Enteric Coronavirus Haemagglutinin Protein

Hogue¹,

Kienzle²,

Brian³

1989

Journal of General Virology

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SUMMARYThe haemagglutinin molecule on the bovine enteric coronavirus has been identified as a glycoprotein of 140K composed of disulphide-linked subunits of 65K. In this study, we have shown the subunits to be identical by demonstrating an unambiguous aminoterminal amino acid sequence. The unglycosylated subunit was found to have an M r of 42.5K and to undergo rapid disulphide linkage and glycosylation. Glycosylation was found to be of the asparagine-linked type and some of the oligosaccharides underwent processing to complex forms. Studies with inhibitors of glycosylation suggested that a processing of the haemagglutinin oligosaccharide takes place on the virion whilst it is in the Golgi apparatus. Each haemagglutinin subunit on the mature virion was estimated to possess six or seven carbohydrate chains of either the high-mannose or hybrid type, and three or four chains of the complex type.

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Section: Methodsmentioning

confidence: 99%

Synthesis and Processing of the Bovine Enteric Coronavirus Haemagglutinin Protein

Hogue¹,

Kienzle²,

Brian³

1989

Journal of General Virology

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“…Stools were obtained from 39 individuals (13 with diarrhoea and 26 controls) on 5, 7, 9 and 11 July. From (Tompkins et al 1974) (kindly supplied by Dr J. Laporte, INRA, Theiverval-Grignon, France) and SKCO-1 and HT-29 derived from human colonic cancers (Fogh & Trempe, 1975) (The Memorial Sloan Kettering Institute, New York).…”

Section: Laboratory Methodsmentioning

confidence: 99%

An epidemic of acute diarrhoea in rural southern India associated with echovirus type 11 infection

Patel

Daniel

Mathan

1985

J. Hyg.

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SUMMARYAn epidemic of diarrhoea with two distinct waves affected a village of 1375 people in southern India in 1983. The first wave of the epidemic, from the last week of December 1982, had a sharp peak in January 1983 and was over by March. Echovirus type 11 was isolated from patients, who also had a serum antibody response to the virus. During the second wave of the epidemic, from May to September 1983, the clinical features were different andShigella flexneriwas isolated without significant viral isolates. Infection during the first wave did not protect from the second wave. Virus isolation was in human intestinal tumourderived differentiated epithelial cell lines; such cell lines may be useful for the isolation and identification of entcroviruses in clinical samples.

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“…The HCT-8 cell line was originally derived from a male patient with an adenocarcinoma of the ileocecal region (Tompkins et al, 1974). Cultures were maintained in RPMI-1640 (Gibco ± BRL, Gent, Belgium), supplemented with 10% heat-inactivated foetal bovine serum, 1 mM sodium pyruvate, 100 mg/ml streptomycin, 250 IU/ml penicillin and 2.5 mg/ml fungizone (hereafter called culture medium).…”

Section: Cellsmentioning

confidence: 99%

The αE-catenin gene (CTNNA1) acts as an invasion-suppressor gene in human colon cancer cells

Vermeulen

Nollet

Teugels³

et al. 1999

Oncogene

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The acquisition of invasiveness is a crucial step in the malignant progression of cancer. In cancers of the colon and of other organs the E-cadherin/catenin complex, which is implicated in homotypic cell-cell adhesion as well as in signal transduction, serves as a powerful inhibitor of invasion. We show here that one allele of the aE-catenin (CTNNA1) gene is mutated in the human colon cancer cell family HCT-8, which is identical to HCT-15, DLD-1 and HRT-18. Genetic instability, due to mutations in the HMSH6 (also called GTBP) mismatch repair gene, results in the spontaneous occurrence of invasive variants, all carrying either a mutation or exon skipping in the second aE-catenin allele. The aE-catenin gene is therefore, an invasionsuppressor gene in accordance with the two-hit model of Knudsen for tumour-suppressor genes.

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Cultural and Antigenic Properties of Newly Established Cell Strains Derived From Adenocarcinomas of the Human Colon and Rectum

Cited by 251 publications

References 0 publications

Synthesis and Processing of the Bovine Enteric Coronavirus Haemagglutinin Protein

Synthesis and Processing of the Bovine Enteric Coronavirus Haemagglutinin Protein

An epidemic of acute diarrhoea in rural southern India associated with echovirus type 11 infection

The αE-catenin gene (CTNNA1) acts as an invasion-suppressor gene in human colon cancer cells

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