Culturable actinomycetes from desert ecosystem in northeast of Qinghai-Tibet Plateau

Ding, Dong; Chen, Guichen; Wang, Bochan; Wang, Qilan; Liu, Demei; Peng, Min; Shi, Peng

doi:10.1007/s13213-012-0469-9

Cited by 38 publications

(24 citation statements)

References 30 publications

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“…BLAST search results implied that these 41 isolates belonged to the genus Streptomyces (33 isolates), Nocardia (7 isolates) and Kribbella (1 isolate). From the sequencing result, it was clear that Streptomyces was found to be the dominant genus in the soil of protected forest ecosystems which was also reported previously 12,19,70,71 .…”

Section: Discussionsupporting

confidence: 82%

Antimicrobial biosynthetic potential and diversity of culturable soil actinobacteria from forest ecosystems of Northeast India

Sharma

Thakur²

2020

Sci Rep

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Actinobacteria is a goldmine for the discovery of abundant secondary metabolites with diverse biological activities. this study explores antimicrobial biosynthetic potential and diversity of actinobacteria from Pobitora Wildlife Sanctuary and Kaziranga National Park of Assam, India, lying in the Indo-Burma mega-biodiversity hotspot. A total of 107 actinobacteria were isolated, of which 77 exhibited significant antagonistic activity. 24 isolates tested positive for at least one of the polyketide synthase type I, polyketide synthase type II or non-ribosomal peptide synthase genes within their genome. their secondary metabolite pathway products were predicted to be involved in the production of ansamycin, benzoisochromanequinone, streptogramin using DoBISCUIT database. Molecular identification indicated that these actinobacteria predominantly belonged to genus Streptomyces, followed by Nocardia and Kribbella. 4 strains, viz. Streptomyces sp. PB-79 (GenBank accession no. KU901725; 1313 bp), Streptomyces sp. Kz-28 (GenBank accession no. KY000534; 1378 bp), Streptomyces sp. Kz-32 (GenBank accession no. KY000536; 1377 bp) and Streptomyces sp. Kz-67 (GenBank accession no. KY000540; 1383 bp) showed ~89.5% similarity to the nearest type strain in EzTaxon database and may be considered novel. Streptomyces sp. Kz-24 (GenBank accession no. KY000533; 1367 bp) showed only 96.2% sequence similarity to S. malaysiensis and exhibited minimum inhibitory concentration of 0.024 µg/mL against methicilin resistant Staphylococcus aureus ATCC 43300 and Candida albicans MTCC 227. This study establishes that actinobacteria isolated from the poorly explored Indo-Burma megabiodiversity hotspot may be an extremely rich reservoir for production of biologically active compounds for human welfare. Since ancient times, mankind has been exploring nature for bioactive organisms to treat different ailments. More than 100000 natural products have been identified in the last 150 years, which include highly assorted chemical classes such as polyketides, alkaloids, non-ribosomal peptides, isoprenoids or phenylpropanoids 1,2. Microbial biodiversity can provide us with the richest and the most versatile reservoir of potentially active natural products. A large portion of genomes from beneficial microbes are dedicated to the production of these valuable natural products. A single microbe is capable of making 30-50 natural product compounds 3. There are approximately 1 million natural products out of which about 25% are biologically active showing either positive activity or toxicity. Among the prospective sources of natural products, bacteria are prolific sources and a vast majority of these products are produced by a phylum of Gram-positive bacteria known as the "Actinobacteria" 4-6. Actinobacteria are diverse group of bacteria with high content of guanosine-cytosine (65-75%) ranging from 2.5-9.7 Mb genome size 7. Among actinobacteria, about 75% of antibiotics such as ivermectin, streptomycin, nystatin and tetracycline are produced by the microbial world's...

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Section: Discussionsupporting

confidence: 82%

Antimicrobial biosynthetic potential and diversity of culturable soil actinobacteria from forest ecosystems of Northeast India

Sharma

Thakur²

2020

Sci Rep

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“…315 from Mongolian desert soil (Kurapova et al, 2012 ). In addition to Streptomyces , strains belonging to Micromonospora, Saccharothrix, Streptosporangium , and Cellulomonas were obtained from the Qinghai-Tibet Plateau (Ding et al, 2013a ), while Micromonospora, Actinomadura , and Nocardiopsis were reported from soda saline soils of the ephemeral salty lakes in Buryatiya (Lubsanova et al, 2014 ).…”

Section: Xerophilic Strains Isolated From Arid Areasmentioning

confidence: 99%

“…In another study, 53 Actinobacteria isolated from the Qinghai-Tibet Plateau were grouped into four RFLP patterns and identified as Streptomyces, Micromonospora, Saccharothrix, Streptosporangium , and Cellulomonas . Most of these strains had the potential to produce active compounds in addition to the detection of NRPS, PKS-I, and PKS-II genes (Ding et al, 2013a ). Hence, the metagenomic analysis of the bioactive secondary metabolites (Schofield and Sherman, 2013 ; Wilson and Piel, 2013 ) can also be assessed in the future, in order to distinguish the chemical potential of drought adapted Actinobacteria and their conserved secondary metabolites biosynthetic pathways.…”

Section: Biologically Active Metabolites Reported From Xerophilic mentioning

confidence: 99%

Actinobacteria from Arid and Desert Habitats: Diversity and Biological Activity

2016

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The lack of new antibiotics in the pharmaceutical pipeline guides more and more researchers to leave the classical isolation procedures and to look in special niches and ecosystems. Bioprospecting of extremophilic Actinobacteria through mining untapped strains and avoiding resiolation of known biomolecules is among the most promising strategies for this purpose. With this approach, members of acidtolerant, alkalitolerant, psychrotolerant, thermotolerant, halotolerant and xerotolerant Actinobacteria have been obtained from respective habitats. Among these, little survey exists on the diversity of Actinobacteria in arid areas, which are often adapted to relatively high temperatures, salt concentrations, and radiation. Therefore, arid and desert habitats are special ecosystems which can be recruited for the isolation of uncommon Actinobacteria with new metabolic capability. At the time of this writing, members of Streptomyces, Micromonospora, Saccharothrix, Streptosporangium, Cellulomonas, Amycolatopsis, Geodermatophilus, Lechevalieria, Nocardia, and Actinomadura are reported from arid habitats. However, metagenomic data present dominant members of the communities in desiccating condition of areas with limited water availability that are not yet isolated. Furthermore, significant diverse types of polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) genes are detected in xerophilic and xerotolerant Actinobacteria and some bioactive compounds are reported from them. Rather than pharmaceutically active metabolites, molecules with protection activity against drying such as Ectoin and Hydroxyectoin with potential application in industry and agriculture have also been identified from xerophilic Actinobacteria. In addition, numerous biologically active small molecules are expected to be discovered from arid adapted Actinobacteria in the future. In the current survey, the diversity and biotechnological potential of Actinobacteria obtained from arid ecosystems, along with the recent work trend on Iranian arid soils, are reported.

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“…Micromonosporae have been isolated from diverse natural habitats (Genilloud 2012), notably from rhizosphere soil (Carro et al 2013b;Thawai et al 2016) from desert locations in China (Ding et al .2013) and from tissues of a broad range of plants, such as Triticum aestivum (Coombs and Franco 2003), Zea mays (Shen et al 2014), and Parathelypteris beddomei (Zhao et al 2017); as well as from nitrogen-fixing root nodules of actinorhizal plants (Trujillo et al 2006;Carro et al 2013a) and legumes (Trujillo et al 2007;Garcia et al 2010;Trujillo et al 2010;Carro et al 2018b).…”

Section: Introductionmentioning

confidence: 99%

Hunting for cultivable Micromonospora strains in soils of the Atacama Desert

Carro

Razmilic

Nouioui

et al. 2018

Antonie van Leeuwenhoek

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Innovative procedures were used to selectively isolate small numbers of Micromonospora strains from extreme hyper-arid and high altitude Atacama Desert soils. Micromonosporae were recognised on isolation plates by their ability to produce filamentous microcolonies that were strongly attached to the agar. Most of the isolates formed characteristic orange colonies that lacked aerial hyphae and turned black on spore formation, whereas those from the high altitude soil were dry, blue-green and covered by white aerial hyphae. The isolates were assigned to seven multi- and eleven single-membered groups based on BOX-PCR profiles. Representatives of the groups were assigned to either multi-membered clades that also contained marker strains or formed distinct phyletic lines in the Micromonospora 16S rRNA gene tree; many of the isolates were considered to be putatively novel species of Micromonospora. Most of the isolates from the high altitude soils showed activity against wild type strains of Bacillus subtilis and Pseudomonas fluorescens while those from the rhizosphere of Parastrephia quadrangulares and from the Lomas Bayas hyper-arid soil showed resistance to UV radiation.

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Culturable actinomycetes from desert ecosystem in northeast of Qinghai-Tibet Plateau

Cited by 38 publications

References 30 publications

Antimicrobial biosynthetic potential and diversity of culturable soil actinobacteria from forest ecosystems of Northeast India

Antimicrobial biosynthetic potential and diversity of culturable soil actinobacteria from forest ecosystems of Northeast India

Actinobacteria from Arid and Desert Habitats: Diversity and Biological Activity

Hunting for cultivable Micromonospora strains in soils of the Atacama Desert

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