Culturability and Survival of Marine, Freshwater and Clinical Pseudomonas aeruginosa

Khan, Nurul H.; Ahsan, Mahbuba; Taylor, William D.; Kogure, Kazuhiro

doi:10.1264/jsme2.me09178

Cited by 37 publications

(14 citation statements)

References 51 publications

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“…Identification of the soil parameters influencing P. aeruginosa survival should be investigated further also taking into account strain origin (human or animal) and intrinsic characteristics (i.e., antibiotic resistance properties) as well as the nature of P. aeruginosa sources (waste water, organic amendment. However we cannot rule out that P. aeruginosa survived whatever the conditions but became non-culturable, as in aquatic environments (Khan et al, 2010). …”

Section: Discussionmentioning

confidence: 94%

Low occurrence of Pseudomonas aeruginosa in agricultural soils with and without organic amendment

Deredjian

Colinon

Hien

et al. 2014

Front. Cell. Infect. Microbiol.

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The occurrence of Pseudomonas aeruginosa was monitored at a broad spatial scale in French agricultural soils, from various soil types and under various land uses to evaluate the ability of soil to be a natural habitat for that species. To appreciate the impact of agricultural practices on the potential dispersion of P. aeruginosa, we further investigated the impact of organic amendment at experimental sites in France and Burkina Faso. A real-time quantitative PCR (qPCR) approach was used to analyze a set of 380 samples selected within the French RMQS (“Réseau de Mesures de la Qualité des Sols”) soil library. In parallel, a culture-dependent approach was tested on a subset of samples. The results showed that P. aeruginosa was very rarely detected suggesting a sporadic presence of this bacterium in soils from France and Burkina Faso, whatever the structural and physico-chemical characteristics or climate. When we analyzed the impact of organic amendment on the prevalence of P. aeruginosa, we found that even if it was detectable in various manures (at levels from 103 to 105 CFU or DNA targets (g drywt)−1 of sample), it was hardly ever detected in the corresponding soils, which raises questions about its survival. The only case reports were from a vineyard soil amended with a compost of mushroom manure in Burgundy, and a few samples from two fields amended with raw urban wastes in the sub-urban area of Ouagadougou, Burkina Faso. In these soils the levels of culturable cells were below 10 CFU (g drywt)−1.

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Section: Discussionmentioning

confidence: 94%

Low occurrence of Pseudomonas aeruginosa in agricultural soils with and without organic amendment

Deredjian

Colinon

Hien

et al. 2014

Front. Cell. Infect. Microbiol.

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“…Possible changes in S. saprophyticus cell wall could differentiate isolates in beach waters. Such differences were demonstrated in marine Pseudomonas aeruginosa [53]. Indeed, PFGE protocols are validated for clinical isolates and may be less suitable for environmental organisms.…”

Section: Resultsmentioning

confidence: 99%

Staphylococcus saprophyticusRecovered from Humans, Food, and Recreational Waters in Rio de Janeiro, Brazil

Sousa

da-Silva

Sorenson

et al. 2017

International Journal of Microbiology

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Staphylococcus saprophyticus is an important agent of urinary tract infection (UTI) in young women, but information about this pathogen in human microbiota and in common environment is lacking. The aim of this study was to characterize S. saprophyticus isolates from genitoanal microbiota of 621 pregnant women, 10 minas cheese packs, and five beaches in Rio de Janeiro city and compare PFGE profiles of these isolates with five UTI PFGE clusters described in this city. We investigated 65 S. saprophyticus isolates from microbiota, 13 from minas cheese, and 30 from beaches and 32 UTI isolates. Antimicrobial resistance was determined by disk diffusion, MIC by agar dilution, and PCR. Erythromycin-resistance genes erm(C), msr(A), msr(B), mph(C), and lin(A) were found in 93% of isolates. Trimethoprim-sulfamethoxazole resistance correlated with dfrG or dfrA genes. Three cefoxitin-resistant isolates carried the mecA gene. All isolates obtained from cheese were susceptible to all antimicrobial agents. Six of 10 pregnant women with >1 isolate had monoclonal colonization. Isolates from pregnant women shared 100% similarity with UTI PFGE cluster types A and E obtained almost 10 years previously, suggesting temporal persistence of S. saprophyticus. Antimicrobial resistance of beach isolates reflected the profiles of human isolates. Taken together, results indicate a shared source for human and environmental isolates.

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“…Pseudomonas aeruginosa is an extremely adaptable opportunistic pathogen of both plants and animals found ubiquitously through soil, freshwater, and marine environments (55). In contrast, P. fluorescens is not considered to be pathogenic and usually exists in a symbiotic relationship within the plant rhizosphere (56).…”

Section: Discussionmentioning

confidence: 99%

Ubiquitin Activates Patatin-Like Phospholipases from Multiple Bacterial Species

et al. 2014

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Phospholipase A 2 enzymes are ubiquitously distributed throughout the prokaryotic and eukaryotic kingdoms and are utilized in a wide array of cellular processes and physiological and immunological responses. Several patatin-like phospholipase homologs of ExoU from Pseudomonas aeruginosa were selected on the premise that ubiquitin activation of this class of bacterial enzymes was a conserved process. We found that ubiquitin activated all phospholipases tested in both in vitro and in vivo assays via a conserved serine-aspartate catalytic dyad. Ubiquitin chains versus monomeric ubiquitin were superior in inducing catalysis, and ubiquitin-like proteins failed to activate phospholipase activity. Toxicity studies in a prokaryotic dual-expression system grouped the enzymes into high-and low-toxicity classes. Toxicity measured in eukaryotic cells also suggested a two-tiered classification but was not predictive of the severity of cellular damage, suggesting that each enzyme may correspond to unique properties perhaps based on its specific biological function. Additional studies on lipid binding preference suggest that some enzymes in this family may be differentially sensitive to phosphatidyl-4,5-bisphosphate in terms of catalytic activation enhancement and binding affinity. Further analysis of the function and amino acid sequences of this enzyme family may lead to a useful approach to formulating a unifying model of how these phospholipases behave after delivery into the cytoplasmic compartment.

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Culturability and Survival of Marine, Freshwater and Clinical Pseudomonas aeruginosa

Cited by 37 publications

References 51 publications

Low occurrence of Pseudomonas aeruginosa in agricultural soils with and without organic amendment

Low occurrence of Pseudomonas aeruginosa in agricultural soils with and without organic amendment

Staphylococcus saprophyticusRecovered from Humans, Food, and Recreational Waters in Rio de Janeiro, Brazil

Ubiquitin Activates Patatin-Like Phospholipases from Multiple Bacterial Species

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