Cultivos celulares primarios de Lutzomyia shannoni (Diptera: psychodidae) y estudio cariologico preliminar de la especie

“…Forty slides were examined, each containing from 3 to 12 karyotypes. The chromosomes were identified according to relative size and centromere position (Sessions 1996).As reported by Bello et al (1997) the complement is eight chromosomes for L. shannoni (Fig. 1A).…”

mentioning

confidence: 95%

Brain cell karyotype of the phlebotomine sand fly Lutzomyia shannoni (Dyar) (Diptera: Psychodidae)

Jiménez

Bello

Ferro

et al. 2001

Mem. Inst. Oswaldo Cruz

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The brain cell karyotype of New World sand fly Lutzomyia shannoni was described. This species has four pairs of chromosomes, 2N=8, with one pair of heteromorphic chromosomes.Key words: Lutzomyia shannoni -brain cell karyotype -heteromorphic chromosome Brain cell karyotypes of four Old World and eight New World sand flies were described and compared by Kreutzer et al. (1987Kreutzer et al. ( , 1988. Among the eight New World sand flies species, the chromosome number varies from 2N=6 in Lutzomyia trapidoi to 2N=8 in the following species:. spinicrassa, and L. longipalpis. Heteromorphic chromosomes were not observed in these species (Kreutzer et al. 1988). On the other hand, Yin et al. (1999) studied patterns of G-banding in mitotic karyotypes in populations of L. longipalpis and found ancestral patterns in the populations. This paper provides preliminary data of cytogenetic studies of New World sand fly L. shannoni.Fourth instar larvae were obtained from a L. shannoni colony maintained since 1992 in the Entomology Laboratory of Instituto Nacional de Salud of Colombia, and originated from collections in the Lower Magdalena Valley. The techniques used for rearing the sand fly followed standard protocols in our laboratory. The larval diet was made with bovine manure and dog food; this mixture was stored for three months and stirred every two weeks. The females were fed on hamster blood (Ferro et al. 1998 Kreutzer et al. (1987). The larvae were placed in 0.1% colchicine for 4 h; then the heads of larvae were removed and placed in a 2% orcein stain for 10 min, 25 ml glacial acetic acid, and 25 ml 85% lactic acid. After staining, the heads were placed in a small drop of 50% acetic acid on a microscope slides. The contens of the head capsule were removed by pressure. Then the pieces of the head capsule were discarded and the small drop of stain was added. The cover slide was placed on the slide and the tissue was squashed in folded filter paper to absorb excess stain/acid. The slides were photographed with an Olympus microscope system at 1000X. Forty slides were examined, each containing from 3 to 12 karyotypes. The chromosomes were identified according to relative size and centromere position (Sessions 1996).As reported by Bello et al. (1997) the complement is eight chromosomes for L. shannoni (Fig. 1A). We observed several karyotypes with one pair, chromosome I, as heteromorphic (Fig. 1B) and probably an X and Y chromosome. Determination of the sex of the larvae was not possible before dissection. Chromosomes I, III and IV were metacentric, whereas chromosome II was submetacentric. This karyotype was similar to that of L. spinicrassa except that in this species the heteromorphic chromosome was not observed (Kreutzer et al. 1987).Only in Old World sand fly Phlebotomus perniciosus has been reported with a pair of chromosome heteromorphic (Kreutzer et al. 1987). The pair of chromosome heteromorphic observed in L. shannoni and P. perniciosus suggest the presence of sex chomosomes in other species of the genera Lutzomyia an...

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Líneas celulares de dípteros: características, aplicaciones y aportes desde Colombia

Jiménez Camacho,

Losada,

Rangel

et al. 2023

Cienc. En Desarro.

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Los cultivos celulares son una herramienta que permite el mantenimiento de células in vitro conservando al máximo sus características fisiológicas, bioquímicas y genéticas, los cuales pueden presentar propiedades muy diversas dependiendo de la especie, tejido de origen y el medio de cultivo empleado. El uso de cultivos celulares de dípteros constituye una metodología valiosa en diferentes disciplinas, tales como fisiología, genética, bioquímica y patología; así, por ejemplo, se han podido estudiar diversas enfermedades tanto de interés en medicina humana como en veterinaria. En la presente revisión se presenta información de múltiples líneas celulares del orden Diptera, las cuales se han empleado para llevar a cabo estudios de interacción célula a célula, flujo intracelular de metabolitos, producción de bioinsecticidas, amplificación viral y producción de péptidos antimicrobiano. En Colombia, se han establecido 12 líneas celulares de dípteros las cuales fueron caracterizadas y estandarizadas, algunas de ellas se han empleado como sustratos en estudios del ciclo biológico de parásitos del género Leishmania, también, en la evaluación a la susceptibilidad de arbovirus. En esta tendencia, se analiza y discute la información de las líneas celulares derivadas de dípteros con el fin de realizar el presente trabajo de revisión sobre sus características y aplicaciones.

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Cultivos celulares primarios de Lutzomyia shannoni (Diptera: psychodidae) y estudio cariologico preliminar de la especie

Cited by 3 publications

References 1 publication

Genetic variability among populations of Lutzomyia (Psathyromyia) shannoni (Dyar 1929) (Diptera: Psychodidae: Phlebotominae) in Colombia

Genetic variability among populations of Lutzomyia (Psathyromyia) shannoni (Dyar 1929) (Diptera: Psychodidae: Phlebotominae) in Colombia

Brain cell karyotype of the phlebotomine sand fly Lutzomyia shannoni (Dyar) (Diptera: Psychodidae)

Líneas celulares de dípteros: características, aplicaciones y aportes desde Colombia

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