Cultivo in vitro de raíces pilosas del arbusto Phyllanthus acuminatus (Phyllanthaceae)

Abstract

Introducción: En condiciones naturales, las raíces del arbusto, Phyllanthus acuminatus, producen bajas concentraciones de metabolitos secundarios de interés medicinal. Esto abre una oportunidad para el cultivo in vitro, para aumentar la concentración de metabolitos. Objetivo: Determinar las condiciones óptimas de cultivo líquido para raíces pilosas de P. acuminatus. Métodos: Se utilizó la evaluación del crecimiento de la biomasa según porcentaje de inóculo inicial (0.50 y 0.10 %), porcentaje de nutrientes de los medios (100, 50 y 25 %) y tasa de agitación (90, 100 y 110 min-1) (N= 15 repeticiones). Resultados: Las mejores condiciones de cultivo líquido fueron: 0.10 % de inóculo inicial, nutrientes al 25 % y 90 min-1 para la tasa de agitación. Hay diferencias entre las raíces pilosas y las raíces no transformadas. Conclusiones: es factible producir raíces pilosas de P. acuminatus a gran escala, aplicando e implementando las condiciones evaluadas de porcentaje de inóculo, nutrientes en el medio y tasas de agitación utilizadas en este estudio.

Evaluation of Salicylic Acid and Methyl Jasmonate as Elicitors in Phyllanthus acuminatus Hairy Roots by Non-Targeted Analysis Using High-Resolution Mass Spectrometry

“…Some of the interesting substances found in roots are secondary metabolites; their concentrations within the plant are low and fluctuate due to geographic, seasonal, and environmental variations, making field plant extracts economically impractical since large land extensions would be needed to recover a reasonable amount of biomass. Thus, the cultivation of in vitro organs through genetic engineering is a feasible alternative for producing phytochemicals of interest [ 6 ].…”

“…In vitro culture of Phyllanthus sp. is feasible since its cultivation does not require complex nutrients, growth medium, or other special conditions [ 6 , 7 ]. Also, hairy root extracts of Phyllanthus amarus were shown to induce apoptotic cell death in human breast cancer cells, indicating that the generated plant material possesses interesting bioactive metabolites [ 8 ].…”

“…On many species, including Phyllanthus pulcher , eliciting compounds such as salicylic acid (SA) or methyl jasmonate (MeJA) ( Figure 1 ) has increased the production of secondary metabolites. Jasmonic acid (JA) and MeJA are the compounds most commonly studied and widely used as excitons for promoting the accumulation of secondary metabolites in horticultural products, improving their nutritional quality [ 6 ]. MeJA has been proven to alter the levels of various primary metabolites, including plant sugars, organic acids, and amino acids [ 11 , 12 ].…”

“…tal variations, making field plant extracts economically impractical since lar sions would be needed to recover a reasonable amount of biomass. Thus, of in vitro organs through genetic engineering is a feasible alternative for p tochemicals of interest [6].…”