Cultivation of the yeast <i>candida lipolytica</i> on hydrocarbons. I. Degradation of <i>n</i>‐alkanes in batch fermentation of gas oil

Dostálek, Milan; Munk, V.; Volfová, O.; Pecka, K.

doi:10.1002/bit.260100103

Cited by 26 publications

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“…Lower values of 50 to 90% weight of substrate utilization were observed for some bacteria (5). The highest cell yields of 110 to 130% were reported for the bacterial genera of Micrococcus and Nocardia (16,22), whereas yeast grown on petroleum give a yield of 80 to 100% (4,13,19). Cell yields differ according to the microorganisms used and hydrocarbon substrate applied in the process.…”

Section: Discussionmentioning

confidence: 99%

Batch Production of Protein from Ethane and Ethane-Methane Mixtures

Volesky¹,

Zajic²

1971

Applied Microbiology

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A culture of Graphium grows upon natural gas and a mineral salt solution. Ethane is the preferred substrate but methane is co-utilized. A stirred-tank type fermentor was used to study batch growth. Maximum production rate of biomass was 80 mg/liter * hr, at pH 4, using simple synthetic supporting medium with ammonium sulfate as a nitrogen source. This rate was observed after 40 hr of fermentation. A doubling time of 3.7 hr was observed. The corresponding specific growth rate was 0.187 per hr. A magnetic drive fermentor was used to study the effect of continuous recycle of gases in a gas-tight system. The rate of oxygen utilization is approximately 2.1 times higher than for ethane. Oxygen must not be allowed to become limiting in recycle gases. The calculated efficiency of overall biomass synthesis averages 30%. Hyphal and unicellular tissue of Graphium contains 52% protein. It compares favorably with standard FAO protein in its content of amino acids.

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Section: Discussionmentioning

confidence: 99%

Batch Production of Protein from Ethane and Ethane-Methane Mixtures

Volesky¹,

Zajic²

1971

Applied Microbiology

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Cultivation of the yeast Candida lipolytica on hydrocarbons. II. One‐stage continuous cultivation on gas oil

Dostálek

Munk

Volfová

et al. 1968

Biotech & Bioengineering

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SummaryContinuous cultivation of the yeast Candida lipolytzca on gas oil was studied from the viewpoint of biomass production and oil deparaffination. Optimum conditions were found a t the dilution rate D = 0.164.19 when biomass productivity 1.7 g/l/hr and yield coefficient y = 0.92 were achieved. At deparaffination to the same freezing point, more than double the production of biomass and deparaffined oil during a given time unit was achieved in a continuous process than in batch cultivation. Consumption of substrate was followed in both cultivation processes and it was confirmed that individual n-alkanes of gas oil were degraded at various rates and yields. Results proved optimum cultivation conditions to depend on concentration and composition of the paraffinic fraction of gas oil used. To achieve these conditions the continuous process may be controlled by choice of suitable dilution rate and concentration of gas oil.

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Clutivation of yeast on gas oil

Munk¹,

Dostálek²,

Volfová³

1969

Biotech & Bioengineering

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The results achieved by the cultivation of the yeast Candida lipolytica on gas oil are referred. By using a distillation fraction of gas oil distilling between 180-4OO0C, containing 10-2070 of n-alkanes, the optimal condition for biomass production and deparaffination were estimated for various dilution rates and various amounts of gas oil in the medium. The main factor, which influences the yield coefficient by hydrocarbon fermentation is the polyauxie of the hydrocarbon substrate. The penetration of dispersed hydrocarbons into the yeast cell is demonstrated on electron micrographs and the velocity and reversibility of this process is estimated by using tritium-traced hexadecane.

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Cultivation of the yeast candida lipolytica on hydrocarbons. I. Degradation of n‐alkanes in batch fermentation of gas oil

Cited by 26 publications

References 4 publications

Batch Production of Protein from Ethane and Ethane-Methane Mixtures

Batch Production of Protein from Ethane and Ethane-Methane Mixtures

Cultivation of the yeast Candida lipolytica on hydrocarbons. II. One‐stage continuous cultivation on gas oil

Clutivation of yeast on gas oil

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