Cultivation of the early chick embryo in vitro

Romanoff, Alexis L.

doi:10.1002/ar.1090870407

Cited by 15 publications

(10 citation statements)

References 8 publications

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“…In the past, investigators have cultured embryos in glass dishes (Assheton, 1896;Fere, 1900;Loisel, 1900;Paton, 1911;Vogelaar and van den Boogert, 1925;Vollmar, 1935;Schmidt, 19371, ceramic dishes (Corner and Richter, 1973), beakers (Romanoff, 1943;Boone, 1963;Palen and Thorneby, 1976), plastic shells (Quisenberry and Dillon, 1962), petri dishes (Williams and Boone, 1961), sandwich bags (Schlesinger, 1966;Elliott and Bennett, 1971), and in sandwich-type plastic wrap in several configurations (Criley, 1971;Dunn, 1974) with varying degrees of success. Only relatively recently, however, has shell-less culture become a useful laboratory technique.…”

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confidence: 99%

Effects of varying chamber construction and embryo pre‐incubation age on survival and growth of chick embryos in shell‐less culture

1981

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Shell-less culture involves culturing chick embryos with associated yolk and albumen outside of the eggshell and shell membranes. The technique allows direct access to and continuous observation of cultured embryos almost to the time of hatching. The plastic wrap/culture tripod technique described in this paper allows normal embryonic growth and differentiation from 48 hours (in ovo pre-incubation age) through at least 10 days of total incubation. As the duration of in ovo pre-incubation is decreased below 36 hours, there is a concomitant increase in the percentage of grossly abnormal embryos associated with decreased survival and retarded growth and differentiation. Survival of embryos pre-incubated for 72 hours through 13 and 18 days of total incubation is greater than 80% and 40%, respectively. The wrap/tripod technique allows substantially better survival and more normal development of cultured embryos than does the petri dish technique of Auerbach et al. (1974). Embryonic growth in 7.8-cm-diameter chambers is significantly greater than in either larger (10.6 cm) or smaller (5.2 cm) diameter chambers. Some gas exchange through the culture chamber walls appears necessary for optimal embryonic survival and growth. Suspending egg contents in either Safeway or Handi-Wrap plastic wrap (both of intermediate permeability) in 7.8-cm tripods resulted in superior growth and/or survival compared to suspension in Silastic sheeting (high permeability), Saran Wrap (low permeability), glass dishes (nonpermeable), or glass dishes lined with an inner layer of Safeway wrap(nonpermeable).

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confidence: 99%

Effects of varying chamber construction and embryo pre‐incubation age on survival and growth of chick embryos in shell‐less culture

1981

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“…This agrees with the observation on Barnett (1982) that exposure of the contents of a fertilized chicken egg to a humid atmosphere after 48 hr of incubation allowed good long-term survival. In contrast to the in vitro cultivation methods of Assheton (1896) or Romanoff (1943), our in ovo transplantation and cultivation method of unincubated blastoderms enables a prolonged normal embryonic development without a distorted expansion of the blastoderm. Moreover, this method has the advantage that the albumen from only a single egg was needed and that gaseous exchange occurs on all sides.…”

Section: Resultsmentioning

confidence: 98%

Autoradiographic Demonstration of the Penetration of Albumen-Derived Material Through the Vitelline Membrane into the Egg Yolk, Exterior to the Avian Blastoderm

Callebaut¹

1983

Poultry Science

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A method enabling in ovo transplantation and cultivation of fertilized, unincubated quail yolks is described. By this technique, nonradioactive yolks were transferred into nonradioactive egg shells filled with in vivo radioactively labeled albumen. With autoradiography it was demonstrated that during early avian blastoderm development, albumen-derived material can penetrate through the vitelline membrane of the acellular rimzone distal to the margin of over-growth.

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“…Romanoff (1943) reported that the capillaries are easily destroyed when beakers are shaken. Romanoff (1943) reported that the capillaries are easily destroyed when beakers are shaken.…”

Section: Resultsmentioning

confidence: 99%

“…Romanoff (1943) (1961) successfully grew embryos in petri dishes and in evaporating dishes. Romanoff (1943) (1961) successfully grew embryos in petri dishes and in evaporating dishes.…”

Section: Introductionmentioning

confidence: 99%

A Method of Growing Chick Embryos In Vitro

Boone

1963

Poultry Science

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Cultivation of the early chick embryo in vitro

Cited by 15 publications

References 8 publications

Effects of varying chamber construction and embryo pre‐incubation age on survival and growth of chick embryos in shell‐less culture

Effects of varying chamber construction and embryo pre‐incubation age on survival and growth of chick embryos in shell‐less culture

Autoradiographic Demonstration of the Penetration of Albumen-Derived Material Through the Vitelline Membrane into the Egg Yolk, Exterior to the Avian Blastoderm

A Method of Growing Chick Embryos In Vitro

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