Cultivation of Retinal Pigment Epithelial Cells from Human Choroidal Neovascular Membranes in Age Related Macular Degeneration

Schlunck, Günther; Martin, Gottfried; Agostini, Hansjürgen; Camatta, Gabriele; Hansen, Lutz L.

doi:10.1006/exer.2001.1148

Cited by 19 publications

(24 citation statements)

References 19 publications

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“…ARPE-19 contains retinal G-protein receptor (RGR) and peropsin as light detecting chromophore. Previous observation of vimentin in RPE cells derived from human choroidal neovascular membranes in AMD, as well as in drusen and melanolipofuscin, supporting our choice of phosphoproteome biomarkers in RPE cells under stress [Schlunck et al, 2002;Crabb et al, 2002;Warburton et al, 2007]. Previously, proteomic analysis of the retina revealed that the expression levels of vimentin and PP2A are significantly increased when C3HeB/FeJ mice (rd1 allele, 12 weeks, photoreceptor degenerated) are exposed under continuous light for 7 days compared to a condition of 12h light/dark cycling exposure [Zhang et al, 2010].…”

Section: Phosphorylation Signaling In the Rpesupporting

confidence: 60%

New Biomarkers in the Retina and RPE Under Oxidative Stress

Jahng¹

2012

Ocular Diseases

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Section: Phosphorylation Signaling In the Rpesupporting

confidence: 60%

New Biomarkers in the Retina and RPE Under Oxidative Stress

Jahng¹

2012

Ocular Diseases

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“…The cultured CNV-derived RPE cells from the 2 patients still featured characteristics of RPE cells, as described before [16,17]. However, both CNV-derived RPE cell lines lost their ability to produce pigment in the cell culture.…”

Section: Discussionmentioning

confidence: 74%

“…The sample acquisition and cell culture methods have previously been described by our group [16,17]. In short, the surgical extraction of CNV membranes was a therapeutic option for patients with advanced neovascular AMD before the availability of anti-VEGF agents for intravitreal injection.…”

Section: Sample Acquisitionmentioning

confidence: 99%

“…The retinotomy was secured by a laser retinopexy and gas endotamponade. The extracted CNV membranes were cultured in DMEM/Ham F-12 medium and characterised for RPE features, such as the expression of the RPE markers cytokeratine 18, vimentin, CRALBP, and RPE65, as well as the absence of glial fibrillary acidic protein or alpha smooth muscle actin [16,17].…”

Section: Sample Acquisitionmentioning

confidence: 99%

“…We previously established that these cell cultures from CNV cells bear morphologic characteristics of human RPE cells, that they express markers for RPE, and that angioregulatory factors are differentially expressed [16,17]. By using CNVderived RPE cells, we had the unique opportunity to study cells from a pathologically altered tissue reflecting disease-associated factors such as age and epigenetic changes.…”

mentioning

confidence: 99%

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Expression of Angiogenic and Inflammatory Factors in Choroidal Neovascularisation-Derived Retinal Pigment Epithelium

Ehlken

Guichard²,

Schlunck³

et al. 2017

Ophthalmic Res

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Purpose: Anti-angiogenic treatment is well established in the management of exudative age-related macular degeneration (AMD), but not sufficient in all patients. The characterisation of factors driving this chronic disease could serve to identify additional treatment options. The purpose of this study was to assess gene expression patterns and distinct changes in cells derived from surgically extracted choroidal neovascularisation (CNV) membranes. Materials and Methods: The expression of >11,000 genes was analysed by means of a microarray in cells cultured from 2 late-stage CNV membranes compared to primary human retinal pigment epithelium (RPE) and ARPE-19 cells. A pathway analysis was performed to identify gene expression patterns associated with exudative AMD. Results: The analysis revealed significant alterations in gene sets associated with inflammatory processes in CNV-derived cells, involving the upregulation of pro-inflammatory factors IL6, C3, and C5, and downregulation of anti-inflammatory complement factor B and complement factor I. Factors associated with angiogenesis, such as VEGFA or ANGPT2, were not significantly regulated in the 2 RPE-derived cell lines. Conclusion: In late-stage CNV membrane-derived RPE, gene expression was shifted towards a pro-inflammatory state. Angiogenesis-associated factors were regulated differently in the 2 CNV-derived RPE membranes. While inflammation seems to be continuously stimulated by RPE associated with late exudative AMD, this appears not to be the case with regard to angioregulatory mechanisms.

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Magnetic Nanoparticles for Tissue Engineering

Ito¹,

Honda

2003

Nanotechnologies for the Life Sciences

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The sections in this article are Introduction Mesenchymal Stem Cell Isolation and Expansion MSC Expansion using MCLs MSC Isolation and Expansion using AMLs Mag‐seeding Construction of 3 D Tissue‐like Structure Cell Sheet Engineering using RGD ‐ MCLs Construction of a Keratinocyte Sheet using MCLs Delivery of Mag‐tissue Engineered RPE Sheet Construction of a Liver‐like Structure using MCLs Construction of Tubular Structures using MCLs Conclusion

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Cultivation of Retinal Pigment Epithelial Cells from Human Choroidal Neovascular Membranes in Age Related Macular Degeneration

Cited by 19 publications

References 19 publications

New Biomarkers in the Retina and RPE Under Oxidative Stress

New Biomarkers in the Retina and RPE Under Oxidative Stress

Expression of Angiogenic and Inflammatory Factors in Choroidal Neovascularisation-Derived Retinal Pigment Epithelium

Magnetic Nanoparticles for Tissue Engineering

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