Cultivation of Pichia capsulata as a whole-cell biocatalyst with NADH-dependent alcohol dehydrogenase activity for R -1-phenylethanol production

Homola, Patrik; Kurák, Tomáš; Illeová, Viera; Polakovič, Milan

doi:10.1016/j.fbp.2015.07.007

Cited by 12 publications

(8 citation statements)

References 26 publications

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“…For the prediction of acetophenone reduction to 1-phenylethanol with immobilized cells, the effective diffusion coefficients of R-1-phenylethanol were approximated by the values of acetophenone effective diffusion coefficients. Kinetic experiments of acetophenone reduction to R-1-phenylethanol by a Pichia capsulata whole-cell biocatalyst (36 g•l -1 ) were carried out at optimal pH (pH 5), temperature of 25 °C and glucose concentration of 50 g•l -1 (Homola et al, 2015). The progress curves of acetophenone and R-1-phenylethanol were applied to collect the kinetic data.…”

Section: Resultsmentioning

confidence: 99%

“…During fermentation, 5 ml of samples were taken every 1.5 hour. Concentration of ethanol in supernatant was measured using HPLC (Agilent, USA) equipped with a refractive index detector (Homola 2015). At the end of cultivation, the biomass was collected by centrifugation (Hettich, Germany) (T = 6 °C, 9000 rpm for 10 min), three times washed with water and mixed with a buffer (0.1 mol•l -1 phosphate, pH 5) in the exact mass ratio of 1 : 3 on an analytical balance.…”

Section: Processing Of Samples and Final Biomass From Cultivationmentioning

confidence: 99%

“…The upper organic phase was removed with a pipette and mixed with Na 2 SO 3 to remove water. Then, the samples were filtered into vials and the concentrations of the acetophenone, R-1-phenylethanol and S-1-phenylethanol were determined using GC (Agilent Technologies, USA) under conditions previously published (Homola, 2015). The enantiomeric excess (e.e) was calculated from known concentrations of each enantiomers using the equation:…”

Section: Reaction Experimentsmentioning

confidence: 99%

“…Among the 23 wild yeasts strains tested, only Pichia capsulata ATCC 16753 meets both required criteria. Concentration of glucose, control of oxygen during cultivation as well as cultivation time contribute to high yield and high enantioselectivity of the mentioned homochiral 1-phenyethanol (Homola et al, 2015). As discussed in the introduction, immobilization of different S-specific microorganisms into hydrogels improved the enantiomeric excess, yield and stabi lity of the catalyst in the cells.…”

Section: Introductionmentioning

confidence: 98%

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Biotransformation of acetophenone to R-1-phenylethanol with immobilized Pichia capsulata in batch reactor

Illeová

Ačai

Féher

et al. 2015

Acta Chimica Slovaca

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Biotransformation of acetophenone to R-1-phenylethanol with Pichia capsulata immobilized in Caalginate gel beads was studied. Experiments were carried out in a batch reactor stirred on an orbital shaker (300 rpm) at 25 ℃. Based on experimental data, uncoupled parameters of reaction kinetics (measurements with free cells) and diffusion coefficients (sorption method) were estimated. The model expressing the conversion of acetophenone with immobilized cells in the batch reactor consisted of mass balance equations of acetophenone and R-1-phenylethanol in the liquid and solid phases. A satisfactory agreement between the experimental and predicted values of acetophenone and R-1-phenylethanol concentrations was achieved

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Section: Resultsmentioning

confidence: 99%

Section: Processing Of Samples and Final Biomass From Cultivationmentioning

confidence: 99%

Section: Reaction Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Biotransformation of acetophenone to R-1-phenylethanol with immobilized Pichia capsulata in batch reactor

Illeová

Ačai

Féher

et al. 2015

Acta Chimica Slovaca

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“…The effectiveness of P. pastoris as a whole cell biocatalyst has been investigated for ester biosynthesis, acetophenone reduction . d ‐Amino acid oxidation and kinetic resolution of racemic α‐methylbenzylamine to 99% ee ( R )‐α‐methylbenzylamine .…”

Section: Introductionmentioning

confidence: 99%

Biotransformation of β‐hydroxypyruvate and glycolaldehyde to l‐erythrulose by Pichia pastoris strain GS115 overexpressing native transketolase

Wei

Braun‐Galleani

Henriquez

et al. 2017

Biotechnology Progress

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Transketolase is a proven biocatalytic tool for asymmetric carbon‐carbon bond formation, both as a purified enzyme and within bacterial whole‐cell biocatalysts. The performance of Pichia pastoris as a host for transketolase whole‐cell biocatalysis was investigated using a transketolase‐overexpressing strain to catalyze formation of l‐erythrulose from β‐hydroxypyruvic acid and glycolaldehyde substrates. Pichia pastoris transketolase coding sequence from the locus PAS_chr1‐4_0150 was subcloned downstream of the methanol‐inducible AOX1 promoter in a plasmid for transformation of strain GS115, generating strain TK150. Whole and disrupted TK150 cells from shake flasks achieved 62% and 65% conversion, respectively, under optimal pH and methanol induction conditions. In a 300 μL reaction, TK150 samples from a 1L fed‐batch fermentation achieved a maximum l‐erythrulose space time yield (STY) of 46.58 g L−1 h−1, specific activity of 155 U normalgCDW−1, product yield on substrate (Yp/s) of 0.52 mol mol−1 and product yield on catalyst (Yp/x) of 2.23g normalgCDW−1. We have successfully exploited the rapid growth and high biomass characteristics of Pichia pastoris in whole cell biocatalysis. At high cell density, the engineered TK150 Pichia pastoris strain tolerated high concentrations of substrate and product to achieve high STY of the chiral sugar l‐erythrulose. © 2017 The Authors Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers Biotechnol. Prog., 34:99–106, 2018

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Rational design of the carbonyl reductase EbSDR8 for efficient biosynthesis of enantiopure (R)-3-chloro-1-phenyl-1-propanol

Shao

Yang

et al. 2020

Appl Microbiol Biotechnol

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Cultivation of Pichia capsulata as a whole-cell biocatalyst with NADH-dependent alcohol dehydrogenase activity for R -1-phenylethanol production

Cited by 12 publications

References 26 publications

Biotransformation of acetophenone to R-1-phenylethanol with immobilized Pichia capsulata in batch reactor

Biotransformation of acetophenone to R-1-phenylethanol with immobilized Pichia capsulata in batch reactor

Biotransformation of β‐hydroxypyruvate and glycolaldehyde to l‐erythrulose by Pichia pastoris strain GS115 overexpressing native transketolase

Rational design of the carbonyl reductase EbSDR8 for efficient biosynthesis of enantiopure (R)-3-chloro-1-phenyl-1-propanol

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