Cultivation of Microorganisms in the Cultural Medium Made from Squid Internal Organs and Accumulation of Polyunsaturated Fatty Acids in the Cells

Morita, Naoki; Ichise, Nobutoshi; Yumoto, Isao; Yano, Yutaka; Ohgiya, Satoru; Okuyama, Hidetoshi

doi:10.1007/s10529-005-7187-3

Cited by 4 publications

(4 citation statements)

References 21 publications

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“…In addition to P2 and P3 domains, it has two subdomains (P1a and P1b) and one domain (P0) highly conserved only in PPTases responsible for the biosynthesis of n − 3 PUFAs. Second, the protein encoded by pfaE could completely replace PPTase of SCRC‐2738, and third, EPA is scarcely produced in MP‐1 [22].…”

Section: Resultsmentioning

confidence: 99%

A phosphopantetheinyl transferase gene essential for biosynthesis of n− 3 polyunsaturated fatty acids from Moritella marina strain MP‐1

et al. 2006

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A phosphopantetheinyl transferase (PPTase) gene (pfaE), cloned from the docosahexaenoic acid (DHA)-producing bacterium Moritella marina strain MP-1, has an open reading frame of 861 bp encoding a 287-amino acid protein. When the pfaE gene was expressed with pfaA-D, which are four out of five essential genes for biosynthesis of eicosapentaenoic acid (EPA) derived from Shewanella pneumatophori SCRC-2738 in Escherichia coli, the recombinant produced 12% EPA of total fatty acids. This suggests that pfaE encodes a PPTase required for producing n À 3 polyunsaturated fatty acids, which is probably involved in the synthesis of DHA in M. marina strain MP-1.

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Section: Resultsmentioning

confidence: 99%

A phosphopantetheinyl transferase gene essential for biosynthesis of n− 3 polyunsaturated fatty acids from Moritella marina strain MP‐1

et al. 2006

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“…Existing studies with MP-1 have used complex media (Morita et al 2005a;Morita et al 2005b). Here we have tested MOPS minimal media, modified to contain 20 g/L NaCl (MMOPS), for its ability to support M. marina MP-1 growth and DHA production at 10, 15 and 20 0 C (Table 1).…”

Section: Defined Media Supports Dha Productionmentioning

confidence: 99%

“…Among the characterized marine isolates, Moritella marina MP-1, originally known as Vibrio marinus, is particularly interesting due to the presence of DHA in its membrane lipids as a fatty acyl component. A systematic comparison of several marine bacteria showed that M. marina MP-1 produced more than twice as much DHA as the other 9 species examined (Morita et al 2005a). A draft genome sequence of this organism was recently announced (Kathaurapu and Jarboe, submitted).…”

Section: Introductionmentioning

confidence: 99%

“…It has been demonstrated that growth in media rich in fatty acids or supplemented with the antibiotic cerulenin increased the overall DHA titer (Morita et al 2005a;Morita et al 2005b). However, the current literature lacks a systematic investigation on the effect of growth media composition, nitrogen and carbon source, media pH, or growth temperature on DHA production by M. marina MP-1.…”

Section: Introductionmentioning

confidence: 99%

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Growth condition optimization for docosahexaenoic acid (DHA) production by Moritella marina MP-1

Kautharapu

Rathmacher

Jarboe

2012

Appl Microbiol Biotechnol

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The marine organism Moritella marina MP-1 produces the polyunsaturated fatty acid docosahexaenoic acid (DHA). While the basic metabolic pathway for DHA production in this organism has been identified, the impact of growth conditions on DHA production is largely unknown. This study examines the effect of supplemental carbon, nitrogen and salts, growth temperature and media composition and pH on DHA and biomass production and the fatty acid profile. The addition of supplemental nitrogen significantly increased the overall DHA titer via an increase in biomass production. Supplemental glucose or glycerol increased biomass production, but decreased the amount of DHA per biomass, resulting in no net change in the DHA titer. Acidification of the baseline media pH to 6.0 increased DHA per biomass. Changes in growth temperature or provision of supplemental sodium or magnesium chloride did not increase DHA titer. This organism was also shown to grow on defined minimal media. For both media types, glycerol enabled more DHA production per biomass than glucose. Combination of these growth findings into marine broth supplemented with glycerol, yeast extract, and tryptone at pH 6.0 resulted in a final titer of 82 Â± 5 mg/L, a nearly eightfold increase relative to the titer of 11 Â± 1 mg/L seen in the unsupplemented marine broth. The relative distribution of other fatty acids was relatively robust to growth condition, but the presence of glycerol resulted in a significant increase in myristic acid (C14:0) and decrease in palmitic acid (C16:0). In summary, DHA production by M. marina MP-1 can be increased more than fivefold by changing the growth media. Metabolic engineering of this organism to increase the amount of DHA produced per biomass could result in additional increases in titer. ABSTRACTThe marine organism Moritella marina MP-1 produces the polyunsaturated fatty acid docosahexaenoic acid (DHA). While the basic metabolic pathway for DHA production in this organism has been identified, the impact of growth conditions on DHA production is largely unknown. This study examines the effect of supplemental carbon, nitrogen and salts, growth temperature and media composition and pH on DHA and biomass production and the fatty acid profile. The addition of supplemental nitrogen significantly increased the overall DHA titer via an increase in biomass production. Supplemental glucose or glycerol increased biomass production, but decreased the amount of DHA per biomass, resulting in no net change in the DHA titer.Acidification of the baseline media pH to 6.0 increased DHA per biomass. Changes in growth temperature or provision of supplemental sodium or magnesium chloride did not increase DHA titer. This organism was also shown to grow on defined minimal media. For both media types, glycerol enabled more DHA production per biomass than glucose. Combination of these growth findings into marine broth supplemented with glycerol, yeast extract and tryptone at pH 6.0 resulted in a final titer of 82+5 mg/L, a nearly 8-fold increase...

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Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella marina MP-1 (ATCC 15381)

Kautharapu

Jarboe

2012

J Bacteriol

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Cultivation of Microorganisms in the Cultural Medium Made from Squid Internal Organs and Accumulation of Polyunsaturated Fatty Acids in the Cells

Cited by 4 publications

References 21 publications

A phosphopantetheinyl transferase gene essential for biosynthesis of n− 3 polyunsaturated fatty acids from Moritella marina strain MP‐1

A phosphopantetheinyl transferase gene essential for biosynthesis of n− 3 polyunsaturated fatty acids from Moritella marina strain MP‐1

Growth condition optimization for docosahexaenoic acid (DHA) production by Moritella marina MP-1

Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella marina MP-1 (ATCC 15381)

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