Cultivation of Medicinal Plants from the Wild Flora in Bulgaria-Possibilities and Prospects

Angelova, Siya; Varbanova, Kana; Peeva, Ivanka; Guteva, Yana; Dimitrova, Dimitriya

doi:10.1300/j044v02n02_02

Cited by 4 publications

(2 citation statements)

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“…The germination percentages of the studied population were higher compared with data in previous studies by Casalini et al (2017) and Benvenuti and Bacci (2010) who reported germination percentages 50% to 60%. The high germination, without any seed pretreatment, soon after seed harvest and for a year after, indicated that there was no dormancy, similarly to other Clinopodium species, as C. sandaliotica (Mattana et al, 2016) and C. vulgare (Angelova et al, 1994). The Lamiaceae family includes species either nondormant or physiologically dormant (Baskin and Baskin, 1998).…”

Section: Resultsmentioning

confidence: 99%

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Vlachou¹,

Papafotiou²,

Bertsouklis³

2019

horts

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Seed ecophysiology and micropropagation of Clinopodium nepeta, an aromatic Mediterranean plant with pharmaceutical and horticultural uses was investigated. The optimum germination temperature of seeds stored at room temperature for 0, 6, or 12 months was 15 to 20 °C (100% germination completed in 10 to14 days) and cardinal temperatures were defined at 10 and 30 °C (80% to 82% and 62% to 76% germination, respectively). Six or 12 months of storage did not seem to affect germination, although 12-month-old seeds germinated at higher percentage and completed germination earlier at 15 °C than at 20 °C. Concerning micropropagation, shoot multiplication at subcultures of both adult plant- and seedling-origin nodal explants was tested on Murashige and Skoog (MS) medium supplemented with various cytokinin types, i.e., zeatin (ZEA), 6-benzyladenine (BA), kinetin (KIN), and 6-γ-γ-(dimethylallylamino)-purine (2IP), at various concentrations from 0.0 to 8.0 mg·L−1. Both explant types presented a rather similar response during in vitro culture. Increasing concentration of all cytokinin types resulted in an increase in shoot number per responding explant (1.1–5.3) and in most cases a decrease in shoot length (0.6–3.4 cm). Increasing cytokinin concentration induced hyperhydricity to a number of shoots (0.1–6.5) per explant, mostly when ZEA and BA were used. Supplementing the MS medium with 8.0 mg·L−1 BA combined with 0.1 mg·L−1 1-naphthaleneacetic acid (NAA) led to almost elimination of hyperhydricity and very satisfactory shoot production (80%/88% explant response and 6.5/7.5 shoot number per responding explant for seedling- / adult-origin explants, respectively). Alternatively, increasing the agar concentration to 12.0 g·L−1 and supplementing the medium with 8.0 mg·L−1 BA only, resulted in the same effect on eliminating hyperhydricity, such as the addition of NAA, and in the best shoot multiplication response achieved in this study (100% explant response, 9.4/9.9 shoots per explant for seedling-/adult-origin explants, respectively). Microshoots rooted abundantly (92% to 100%) on half-strength MS medium, either Hf or supplemented with 0.5 mg·L−1 to 4.0 mg·L−1 indole-3-butyric acid (IBA). The addition of IBA to the rooting medium, regardless of its concentration, affected only the root length by increasing it 2- to 3-fold. Microshoot clusters produced on multiplication media rooted at 96% when cultured on Hf half-strength MS medium. Rooted microshoots and shoot clusters survived at 80% to 100%, respectively, after ex vitro acclimatization in peat:perlite 1:1 (v/v).

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Section: Resultsmentioning

confidence: 99%

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Vlachou¹,

Papafotiou²,

Bertsouklis³

2019

horts

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“…The high germination observed soon after seed harvest and for at least one year after, without any seed pretreatment, indicates absence of dormancy, something that characterizes relative species as well, i.e. C. nepeta (Vlachou et al, 2019), C. vulgare (Angelova et al, 1994) and C. sandaliotica (Mattana et al, 2016), a number of other Lamiaceae plants of Crete Origanum dictamnus, Sideritis syriaca ssp. syriaca, Salvia pomifera ssp.…”

Section: Germinationmentioning

confidence: 99%

Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica

Vlachou

Papafotiou

Bertsouklis

2020

Not Bot Horti Agrobo

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The optimum range of temperature for germination (96-100%) of Calamintha cretica, an herb with potential pharmaceutical and horticultural uses, was 15 to 20 °C, with 10 and 30 °C cardinal temperatures. Storage up to one year did not affect germination. The effect of zeatin (ZEA), 6-benzyladenine (BA), kinetin, and 6-γ-γ-(dimethylallylamino)-purine added in MS medium at concentrations from 0.0 to 8.0 mg L-1 was tested for shoot proliferation of both adult- and seedling-origin nodal explants at first- and sub-culture. Both explant types responded similarly during in vitro culture. At cytokinin concentrations up to 1 mg L-1 explant response was high (over 85%) but shoot number per explant was low (1.2-2.2). Increasing cytokinin from 2.0 to 8.0 mg L-1 resulted to an analogous decrease of explant response and shoot length, and an increase of shoot number, particularly when ZEA or BA was used (5.0-6.6 shoots per explant, 0.5-1.0 cm long) with simultaneous though increase of hyperhydricity (up to 50%). The addition of 0.1 mg L-1 naphthaleneacetic acid into the 8.0 mg L-1 BA medium almost eliminated hyperhydricity and increased explant response, while the increase of agar concentration from 8.0 to 12.0 g L-1 eliminated hyperhidricity and induced the highest shoot proliferation (93-95% explant response, 11.2-12.3 shoots per explant, 0.8-1.0 cm long). Microshoots and microshoot clusters rooted (88-96%) on half-strength MS medium either hormone free or supplemented with 1 to 4 mg L-1 indole-3-butyric acid. Plantlets survived at 80% to 100% after ex vitro acclimatization in peat: perlite 1:1 (v/v).

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Feasibility of Electrochemical Fingerprinting for Plant Phylogeography Study: A Case of Chimonanthus praecox

Lu¹

2020

International Journal of Electrochemical Science

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Investigation of plant relationships is very important in phylogeny and plant culture. In this work, we proposed an electrochemical fingerprint-based method for the investigation of Chimonanthus praecox from different locations. This is the first time that electrochemical fingerprinting technology has been applied to the study of the relationships between subspecies. Three buffer solutions were used as a solvents along with an electrolyte during the Chimonanthus praecox leaf extraction and fingerprint recording. The voltammetric curves of Chimonanthus praecox from different locations exhibited very similar profiles, confirming the feasibility of species identification based on the electrochemical fingerprint. A two-dimensional scatter pattern deduced from two sets of fingerprints was used to distinguish the locations. In addition, this phylogeographic study based on electrochemical fingerprinting suggested that Chimonanthus praecox has multisite and multitime origins.

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Cultivation of Medicinal Plants from the Wild Flora in Bulgaria-Possibilities and Prospects

Cited by 4 publications

References 0 publications

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica

Feasibility of Electrochemical Fingerprinting for Plant Phylogeography Study: A Case of Chimonanthus praecox

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