Cultivation of <i>Mycobacterium Paratuberculosis</i> from Bovine Fecal Specimens and a Suggested Standardized Procedure

Whipple, Diana L.; Callihan, Donald R.; Jarnagin, Jerald L.

doi:10.1177/104063879100300424

Cited by 130 publications

(94 citation statements)

References 20 publications

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“…This study reports the results of an investigation on MAP infection in Korean black goat by using ELISA, faecal culture and molecular assays. Whipple et al (1991). Two grams of each sample were homogenized and decontaminated with hexadecylpyridinium (HPC) 0.9% overnight, and were centrifuged at 3500 x g for 20 min.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Mycobacterium avium subspecies paratuberculosis in Korean black goat (Capra hircus aegagrus)

et al. 2015

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Isolation and characterization of RESUMENLa paratuberculosis, causada por Mycobacterium avium subsp. paratuberculosis (MAP), es una infección crónica e incurable del tracto intestinal de los animales. El estudio se realizó en 2012, usando muestras procedentes de 491 cabras negras coreanas, menores de 2 años, procedentes de un rebaño caprino de la región Sur de Corea. Los animales se evaluaron para detectar presencia de paratuberculosis. Los métodos utilizados para investigar las infecciones por MAP fueron ensayo por inmunoabsorción ligado a enzimas y cultivos microbiológicos. De las 491 cabras de 100 rebaños seleccionados, 4 (0,8%) y 3 (0,6%) dieron resultados positivos para MAP en la prueba de ELISA y el estudio microbiológico de coprocultivo, respectivamente. Las colonias aisladas fueron confirmadas como MAP por IS900-PCR y su tipificación se realizó por medio del ensayo de IS1311 PCR-REA. Los resultados revelaron una baja tasa de prevalencia de infecciones por MAP de cepas de "tipo bovino" y "tipo bisonte".Palabras claves: cabra, Mycobacterium avium subsp. paratuberculosis, cultivo. SUMMARYParatuberculosis, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic incurable infection of intestinal tract of animals. The study took place in 2012, on 491 Korean black goats, older than 2 years, from goat herds located in Southern region, Korea. The animals were sampled and tested for paratuberculosis. Enzyme-linked immunosorbent assay and microbiological culture were used to investigate MAP infection. Among the 491 goats from 100 herds screened, 4 (0.8%) and 3 (0.6%) goats were defined positive for MAP by ELISA and faecal culture, respectively. Isolated colonies were confirmed as MAP by IS900-PCR, and genotyped by IS1311 PCR-REA assays. The results revealed MAP infection at a low prevalence rate with "cattle type" and "bison type" genotypes.

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Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Mycobacterium avium subspecies paratuberculosis in Korean black goat (Capra hircus aegagrus)

et al. 2015

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“…However, the bacterial growth is slow with test results being available only after months of incubation and sampling of multiple tissues may be required to establish the infection status of an animal [43]. However, it is also widely recognized that fecal culture results would severely underestimate true infection status and the fecal cultures have a high level of test failures due to the contaminating bacteria in the feces and irregular shedding [44]. Pooling of fecal samples (e.g.…”

Section: Diagnosis/current Testing Optionsmentioning

confidence: 99%

Paratuberculosis: Diagnostic Methods and their Constraints

Mohana¹,

P²

2015

J Veterinar Sci Technol

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Paratuberculosis (Johne's disease) is a chronic intestinal disease of ruminants and the disease is caused by Mycobacterium avium subspecies paratuberculosis (MAP). The disease has a prolonged pre-patent period and the clinical symptoms of the disease are apparent only during the later stages of the disease. However, the infected animal can shed the organism much before the clinical disease with the risk of spreading the disease to other susceptible animals. The disease manifestation is in four stages with each of these stages requiring a different diagnostic method. During the early stages of the disease Cell mediated immune response (CMI) is higher, and shedding of the organism and serum antibody response is absent or minimized. Delayed type hypersensitivity (DTH) response upon injection of Johnin purified protein derivative (Johnin PPD) in the skin of the animal and interferon gamma release assay (IGRA) is the common CMI based tests for the diagnosis of paratuberculosis. As the disease progress, CMI response reduces and humoral response predominates with progressively increased shedding of the organism. ELISA based tests are commercially available to detect the MAP specific antibodies in serum or milk, and nucleic acid detection methods are used to identify the organism. However, all these CMI and humoral antibody based tests have advantages and disadvantages. It is essential to apply a combination of these tests to detect or rule out the disease in farms of endemic countries.

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“…Os meios para cultivo podem ser líquidos, como o caldo Middlebrook 7H9, ou sólidos que incluem Lowenstein-Jensen, LowensteinJensen modificado e meio Herrold gema de ovo (Herrol's Egg Yolk Medium -HEYM) (WhiPPle et al, 1991). Independentemente do meio usado para o cultivo, todos exigem a suplementação com micobactina J (thorel et al, 1990), um composto que auxilia na captação de ferro pelo micro-organismo.…”

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Paratuberculose em pequenos ruminantes domésticos

et al. 2012

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RESUMOMycobacterium avium subesp. paratuberculosis (MAP) é o agente etiológico da paratuberculose em ruminantes domésticos e silvestres. Em caprinos e ovinos causa uma enterite granulomatosa crônica com emagrecimento progressivo seguido de morte, raramente a diarreia é observada. Nesse aspecto, a doença é considerada uma ameaça mundial aos rebanhos, pois pode permanecer no estádio subclínico por anos, manifestando perdas indiretas na produção animal e na disseminação do agente. Além disso, existem relatos da possível relação de MAP com a doença de Crohn, determinando, assim, a sua relevância na saúde pública. O Brasil não dispõe de dados quantificando as reais perdas produtivas nos rebanhos acometidos pela doença, e poucas informações do comprometimento de caprinos e ovinos no país são relatadas. Assim, este artigo busca revisar a paratuberculose em pequenos ruminantes domésticos. PALAVRAS-CHAVE:Mycobacterium avium subesp. paratuberculosis, ovinos, caprinos. ABSTRACT PARATUBERCULOSIS IN SMALL DOMESTIC RUMINANTS. Mycobacterium avium subsp.paratuberculosis (MAP) is the causative agent of paratuberculosis in domestic and wild ruminants. In sheep and goats it causes a chronic granulomatous enteritis with progressive emaciation and death, diarrhea rarely being observed. In this aspect, the disease is considered a worldwide threat to livestock, as it may remain subclinical for years, with indirect losses in production and agent dissemination. In addition, there are reports of a possible relationship with the agent of Crohn's disease, so it is also of relevance to public health. Brazil does not have any data quantifying the actual production losses in herds affected by the disease, and few details of the involvement of goats and sheep in the country have been reported. Thus, this paper aims to review the occurrence of paratuberculosis in flocks of domestic small ruminants. KEY WORDS: Mycobacterium avium subsp. paratuberculosis, sheep, goats.A paratuberculose é uma doença bacteriana contagiosa crônica do trato intestinal que afeta predominantemente ruminantes domésticos e silvestres (Chiodini et al., 1984;Clarke, 1997; ayele et al., 2001;Mota et al., 2010), causada pelo Mycobacterium avium subesp. paratuberculosis (MAP), da família Mycobacteriaceae. Estes são bastonetes aeróbicos, imóveis, catalase positiva, sendo considerados Gram positivos (tiMMs et al., 2011). Por apresentarem grande quantidade de ácido micólico e lipídios na parede celular, coram-se melhor pela técnica de Ziehl-Neelsen, onde a fucsina carbólica se liga aos lipídeos da parede celular, resistindo ao descorante álcool-ácido, sendo classificados como bacilos álcool-ácido resistentes (BAAR) (Quinn et al., 2005). Essa característica lipídica em sua parede celular confere elevada resistência no meio ambiente. Whittington et al. (2004) demonstraram que MAP pode permanecer viável em solos sombreados por mais de 55 semanas, sendo resistentes à maioria dos desinfetantes comumente utilizados (Collins, 2003). Recentemente, foi verificado que MAP pode também uti...

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Cultivation of Mycobacterium Paratuberculosis from Bovine Fecal Specimens and a Suggested Standardized Procedure

Cited by 130 publications

References 20 publications

Isolation and characterization of Mycobacterium avium subspecies paratuberculosis in Korean black goat (Capra hircus aegagrus)

Isolation and characterization of Mycobacterium avium subspecies paratuberculosis in Korean black goat (Capra hircus aegagrus)

Paratuberculosis: Diagnostic Methods and their Constraints

Paratuberculose em pequenos ruminantes domésticos

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