Cultivation of Bordetella pertussis on agar media

Heptakis(2,6-O-dimethyl)beta-cyclodextrin: a novel growth stimulant for Bordetella pertussis phase I

“…However, they are sensitive to some growth inhibitors such as peptone, sulfur, peroxide, manganese, and fatty acids (12)(13)(14)(15). The effects of these inhibitors can be partially overcome by the addition of blood, albumin, charcoal, soluble starch, or anion-exchange resins to the medium, which serves to inactivate or to sequester one or more inhibitors (2,11,16,20).…”

Effects of fatty acids on growth of Bordetella pertussis in defined medium

“…Bordetella pertussis is a fastidious, slowgrowing bacterium which is difficult to isolate on laboratory medium. Freshly isolated strains are known to be susceptible to a number of inhibitors present in both solid and liquid media, including peptone, sulfur, peroxides, manganese, and fatty acids (3,(10)(11)(12)(13)(14)18). This inhibition has been overcome by the addition of blood, albumen, charcoal, starch, or anion exchange resins to the medium, which serves to neutralize or bind inhibitors (10-15, 17, 18).…”

Use of Glutamic Acid to Supplement Fluid Medium for Cultivation of Bordetella pertussis

“…There have been very few attempts to increase the yield of cells from fluid media by adding extra amino acids, and those which have been reported met with only limited success (4, 7,13,14,16,20,21). In an attempt to improve the economics of vaccine production, it seemed pertinent to determine whether the cessation of cell growth in Cohen and Wheeler broth (2) was due simply to exhaustion of the energy source and, if so, whether the cell yield could be improved by adding extra amino acids to the medium.…”