Cultivation of Avian Encephalomyelitis Virus in vitro. 1. In Chick Embryo Neuroglial Cell Culture

Avian encephalomyelitis: A review

“…Virus was detected for up to 25 days in CEF and 7 days in CEK cultures, and maximum titres were obtained that were approximately lO 2 EID 5 o less than that reported for CEB cell cultures (Mancini & Yates, 1967) with an eclipse phase of 3 days. Despite growth of the virus, no visible c.p.e.…”

“…However, in a subsequent report, Burke et al (1965; quoting unpublished data from R. E. Luginbuhl) indicated that Hwang and others had, in fact, propagated chicken embryo lethal organ (CELO) virus and not AEV. Mancini & Yates (1967) reported multiplication of the egg-adapted Van Roekel strain in cultures of chicken embryo brain (CEB) cells, using inoculation of susceptible chicken embryos to detect cultured virus. Titres of up to 10 50 EIDso/ml were obtained 15 days p.i.…”