Cultivation of Avian Encephalomyelitis Virus in vitro. 2. In Chick Embryo Fibroblastic Cell Culture

Mancini, L; Yates, Vance J.

doi:10.2307/1588227

Cited by 9 publications

(2 citation statements)

References 4 publications

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“…Chicken embryo fibroblast (CEF) and chicken embryo kidney (CEK) cultures were shown to support multiplication of AEV to low titre (Mancini & Yates, 1968, 1986. Virus was detected for up to 25 days in CEF and 7 days in CEK cultures, and maximum titres were obtained that were approximately lO 2 EID 5 o less than that reported for CEB cell cultures (Mancini & Yates, 1967) with an eclipse phase of 3 days.…”

Section: Propagation Of Aev In Cell Culturementioning

confidence: 99%

Avian encephalomyelitis: A review

Tannock

Shafren

1994

Avian Pathology

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Section: Propagation Of Aev In Cell Culturementioning

confidence: 99%

Avian encephalomyelitis: A review

Tannock

Shafren

1994

Avian Pathology

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“…Chicken embryo brain (CEB) and fibroblast (CEF) cell cultures were prepared as described by Sato et al (1971) and Mancini & Yates (1968), respectively, using 11-day-old specified-pathogen-free (SPF) embryonating chicken eggs (Line M, Nisseiken Co., Ltd., Tokyo, Japan). Cells were grown at 37°C in a humidified 5% CO 2 incubator in a growth medium consisting of Eagle's minimal essential medium and 5% foetal calf serum.…”

Section: Cell Culturesmentioning

confidence: 99%

Detection of avian encephalomyelitis viral antigen with a monoclonal antibody

et al. 1994

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SUMMARYA monoclonal antibody (MAb), designated VR9-1, prepared against avian encephalomyelitis virus (AEV) reacted with the embryo-adapted strain, Van Roekel, two vaccine and two field strains in indirect immunofluorescent and immunoperoxidase staining of frozen sections of chicken brain, chicken embryo fibroblast and brain cells inoculated with each virus. The MAb was also used as a detector in avidin-biotin-peroxidase staining in paraffin sections where results indicate that it recognized a common epitope among strains of AEV and may be useful for detection and quantitative studies.

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Dynamic distribution and tissue tropism of avian encephalomyelitis virus isolate XY/Q-1410 in experimentally infected Korean quail

Fan

Huang

et al. 2017

Arch Virol

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Avian encephalomyelitis (AE) is an important infectious poultry disease worldwide that is caused by avian encephalomyelitis virus (AEV). However, to date, the dynamic distribution of AEV in quails has not been well described. Quantitative real-time polymerase chain reaction (qPCR) and immunohistochemistry (IHC) assays were used to investigate the dynamic distribution and tissue tropism of AEV in experimentally infected Korean quail. AEV was detected in the cerebrum, cerebellum, proventriculus, intestine, liver, pancreas, spleen, bursa, lung and kidney as early as 3 days post-infection (dpi). The viral loads in the proventriculus, intestine, spleen and bursa were relatively higher than in other tissues. According to the qPCR results, AEV XY/Q-1410 infection lasted for at least 60 days in infected Korean quail. Immunohistochemistry-positive staining signals of AEV antigen were analysed by Image-Pro Plus software. A positive correlation between qPCR and IHC results was identified in most tissues. Our results provide an insight into the dynamic distribution of AEV in various tissues after infection. The distinct dynamic distribution of the viral genome in Korean quail in the early and late stages of infection suggests that AEV replication is affected by antibody levels and the maturity of the immune system of the host.

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Cultivation of Avian Encephalomyelitis Virus in vitro. 2. In Chick Embryo Fibroblastic Cell Culture

Cited by 9 publications

References 4 publications

Avian encephalomyelitis: A review

Avian encephalomyelitis: A review

Detection of avian encephalomyelitis viral antigen with a monoclonal antibody

Dynamic distribution and tissue tropism of avian encephalomyelitis virus isolate XY/Q-1410 in experimentally infected Korean quail

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