Cultivation of Arthrospira (Spirulina) platensis by Fed-Batch Process

Carvalho, João Carlos Monteiro de; Bezerra, Raquel Pedrosa; Matsudo, Marcelo Chuei; Sato, Sunao

doi:10.1007/978-1-4614-3348-4_33

Cited by 11 publications

(14 citation statements)

References 87 publications

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“…( 3) Air pump; ( 4) 20 W Fluorescent lamps; ( 5) Airlift system (Carvalho et al 2013 The tubular design allowed the possibility of overcoming some of the problems, such as contamination, pH control, harvesting and introduction of nutrients, but light intensity, mixing, aggregation and temperature control are not resolved yet.…”

Section: Characteristics Of Photobioreactorsmentioning

confidence: 99%

“…Careful control of pH and other physical conditions for introducing CO 2 into the ponds allow for more than 90 % utilization of injected CO 2 (Demirbas and Demirbas 2010), and it is also important for optimizing the uptake of ammoniacal nitrogen sources, like ammonium salts and urea, when they are employed (Ávila-Leon et al 2012;Rodrigues et al 2011;Carvalho et al 2013). …”

Section: Ph Controlmentioning

confidence: 99%

“…It is known that the preferred nitrogen supply is in the form of ammonia or urea, either of which is economically more favorable than nitrate, which is more expensive and requires considerable metabolic energy for its assimilation (Sassano et al 2004;Carvalho et al 2013). It is generally assumed that, before assimilation, nitrate-nitrogen is reduced to ammonium-N in two reducing steps catalyzed by the enzymes nitrate reductase and nitrite reductase (Hattori and Myers 1966).…”

Section: Nutrientsmentioning

confidence: 99%

“…The culture volume may vary, depending on the nutrient concentration and evaporation of the system (Carvalho et al 2013). The culture volume may vary, depending on the nutrient concentration and evaporation of the system (Carvalho et al 2013).…”

Section: Cultivation Processesmentioning

confidence: 99%

“…Fed-batch operations can be the best option for some systems in which the nutrients or any other substrates are only sparingly soluble or are too toxic to add the whole requirement for a batch process at the start (Carvalho et al 2013). In this case, it was used to prevent or reduce substrate-associated growth inhibition by controlling nutrient supply.…”

Section: Cultivation Processesmentioning

confidence: 99%

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Microalgae Bioreactors

Carvalho

Matsudo

Bezerra

et al. 2013

Algal Biorefineries

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Photobioreactor design and operation mode are essential steps to ensure a high overall microalgae yield and cell productivities, making viable the commercial production. For this reason, there are trends of research in the field of microalgae that encompass design and development of reactor systems towards maximum productivity with minimum operation costs. In the literature, various photobioreactor designs have been employed such as open ponds, bubble column, flat plate, and tubular (conical, helical, etc.). Open ponds are the most commonly applied photobioreactor design in industrial processes. On the other hand, studies have been focused on tubular photobioreactors due to the possibility of achieving high volumetric productivity and better biomass quality. Therefore, in this chapter, some photobioreactor designs and their characteristics such as geometrical configuration, building material, and cell circulation systems will be discussed. Moreover, the operation mode, such as temperature and pH control, nutrient feeding, CO 2 addition systems, flow rate, light supply, mixing, cultivation process and cleanness will also be considered to be important parameters in this field.

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Section: Characteristics Of Photobioreactorsmentioning

confidence: 99%

Section: Ph Controlmentioning

confidence: 99%

Section: Nutrientsmentioning

confidence: 99%

Section: Cultivation Processesmentioning

confidence: 99%

Section: Cultivation Processesmentioning

confidence: 99%

See 3 more Smart Citations

Microalgae Bioreactors

Carvalho

Matsudo

Bezerra

et al. 2013

Algal Biorefineries

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Semicontinuous system for the production of recombinant mCherry protein in Chlamydomonas reinhardtii

Arias

Matsudo

Ferreira-Camargo

et al. 2020

Biotechnology Progress

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Biotechnology advances have allowed bacteria, yeasts, plants, mammalian and insect cells to function as heterologous protein expression systems. Recently, microalgae have gained attention as an innovative platform for recombinant protein production, due to low culture media cost, compared to traditional systems, as well as the fact that microalgae such as Chlamydomonas reinhardtii are considered safe (GRAS) by the Food and Drug Administration (FDA). Previous studies showed that recombinant protein production in traditional platforms by semicontinuous process increased biomass and bio product productivity, when compared to batch process. As there is a lack of studies on semicontinuous process for recombinant protein production in microalgae, the production of recombinant mCherry fluorescent protein was evaluated by semicontinuous cultivation of Chlamydomonas reinhardtii in bubble column photobioreactor. This semicontinuous cultivation process was evaluated in the following conditions: 20%, 40%, and 60% culture portion withdrawal. The highest culture withdrawal percentage (60%) provided the best results, as an up to 161% increase in mCherry productivity (454.5 RFU h −1 -Relative Fluorescence Unit h −1 ), in comparison to batch cultivation (174.0 RFU h −1 ) of the same strain. All cultivations were carried out for 13 days, at pH 7, temperature 25 C and, by semicontinuous process, two culture withdrawals were taken during the cultivations. Throughout the production cycles, it was possible to obtain biomass concentration up to 1.

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