Cultivation of an Ovine Strain of Ehrlichia phagocytophila in Tick Cell Cultures

Woldehiwet, Z.; Horrocks, B. K.; Scaife, Helena; Ross, Gordon D.; Munderloh, Ulrike G.; Bown, Kevin; Edwards, Steven W.; Hart, C. A.

doi:10.1053/jcpa.2002.0574

Cited by 42 publications

(32 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Organisms derived from IDE8 cells remained infective, causing clinical ehrlichiosis in dogs; a cell line derived from the natural vector, Rhipicephalus sanguineus, did not become infected in vitro [19]. Ehrlichia equi, the human granulocytic ehrlichiosis agent, and Ehrlichia phagocytophila, all recently re-classified as Anaplasma phagocytophilum, have been successfully propagated in the I. scapularis cell lines IDE8 or ISE6 or both [20][21][22]. Both the equine [20] and ovine [22] variants derived from tick cell cultures remained infective for their respective mammalian hosts, and antigen prepared from A. phagocytophilum-infected IDE8 cells was as sensitive and specific in ELISA as infected ovine granulocyte antigen [23].…”

Section: Propagation Of Ehrlichia and Anaplasmamentioning

confidence: 99%

“…Ehrlichia equi, the human granulocytic ehrlichiosis agent, and Ehrlichia phagocytophila, all recently re-classified as Anaplasma phagocytophilum, have been successfully propagated in the I. scapularis cell lines IDE8 or ISE6 or both [20][21][22]. Both the equine [20] and ovine [22] variants derived from tick cell cultures remained infective for their respective mammalian hosts, and antigen prepared from A. phagocytophilum-infected IDE8 cells was as sensitive and specific in ELISA as infected ovine granulocyte antigen [23]. The first continuous propagation of Ehrlichia ruminantium was carried out in IDE8 cells [24] and subsequently in cell lines from other non-vector species R. appendiculatus, Boophilus decoloratus * , B. microplus and I. ricinus and the vector species Amblyomma variegatum (Figure 1c,d) [25][26][27].…”

Section: Propagation Of Ehrlichia and Anaplasmamentioning

confidence: 99%

See 1 more Smart Citation

Tick cell lines: tools for tick and tick-borne disease research

Bell‐Sakyi

Zweygarth

Blouin

et al. 2007

Trends in Parasitology

161

176

View full text Add to dashboard Cite

Section: Propagation Of Ehrlichia and Anaplasmamentioning

confidence: 99%

Section: Propagation Of Ehrlichia and Anaplasmamentioning

confidence: 99%

Tick cell lines: tools for tick and tick-borne disease research

Bell‐Sakyi

Zweygarth

Blouin

et al. 2007

Trends in Parasitology

161

176

View full text Add to dashboard Cite

“…Anaplasma phagocytophilum infects granulocytes and causes a variety of clinical signs, including human granulocytic anaplasmosis, fever and loss of milk yield in cattle, fever in small ruminants and fever, edemas, and petechia in horses (16,18,23,25).…”

mentioning

confidence: 99%

Serologic Cross-Reactivity between Anaplasma marginale and Anaplasma phagocytophilum

Dreher

Fuente

Hofmann‐Lehmann

et al. 2005

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

In the context of a serosurvey conducted on the Anaplasma marginale prevalence in Swiss cattle, we suspected that a serological cross-reactivity between A. marginale and A. phagocytophilum might exist. In the present study we demonstrate that cattle, sheep and horses experimentally infected with A. phagocytophilum not only develop antibodies to A. phagocytophilum (detected by immunofluorescent-antibody assay) but also to A. marginale (detected by a competitive enzyme-linked immunosorbent assay). Conversely, calves experimentally infected with A. marginale also developed antibodies to A. phagocytophilum using the same serological tests. The identity of 63% determined in silico within a 209-amino-acid sequence of major surface protein 5 of an isolate of A. marginale and one of A. phagocytophilum supported the observed immunological cross-reactivity. These observations have important consequences for the serotesting of both, A. marginale and A. phagocytophilum infection of several animal species. In view of these new findings, tests that have been considered specific for either infection must be interpreted carefully.

show abstract

“…Successful initiation and maintenance of A. phagocytophilum in the I. scapularis tick cell lines IDE8 and/or ISE6 has been reported (MUNDERLOH et al, 1996;WOLDEHIWET et al, 2002). Although tick cell lines are not routinely used for direct isolation of A. phagocytophilum, it was shown that organisms derived from infected HL60 cells can invade and grow in tick cell cultures (MUNDERLOH et al, 1999).…”

Section: Culture Initiation and Propagation Of Anaplasma Phagocytophilummentioning

confidence: 99%

“…Cultures are initiated by adding infected granulocytes from venous blood of infected animals into tick cell cultures. Whole blood (WOLDEHIWET et al, 2002), washed buffy coat cells (MUNDERLOH et al, 1999) or white blood cells after hypotonic lysis of the erythrocytes (MUNDERLOH et al, 1996;ZWEYGARTH et al, 2010) can be used for initiation. Culture propagation is basically the same as with A. marginale, including culture medium and incubation temperature.…”

Section: Culture Initiation and Propagation Of Anaplasma Phagocytophilummentioning

confidence: 99%

In vitro cultivation of Anaplasma marginale and A. phagocytophilum in tick cell lines: a review

Passos¹

2012

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

Continuous cell lines have been established from several ixodid and argasid tick species, representing an excellent tool suitable for the isolation of pathogens and their subsequent propagation, which in turn allows the production of antigenic material for diagnostic tests, antibody and vaccine production, and also for studies on host-vector-pathogen relationships. This paper reviews the use of tick cells for culture initiation and maintenance of two obligate intracellular bacterial pathogens, Anaplasma marginale and Anaplasma phagocytophilum. These in vitro cultivation systems have been used in a wide range of studies, covering morphological ultrastructural analysis, genetics, proteomics and biological differences between strains, including genome transcriptional and protein expression approaches, enabling comparisons between host and vector cells. Thus, such systems open a new window for a better understanding of interactions between pathogens and tick cells. Last but not least, such systems contribute to the reduction in usage of animals for experimental research, as antigenic material can be produced in reasonably large quantities without the use of in vivo species-specific systems.

show abstract

Cultivation of an Ovine Strain of Ehrlichia phagocytophila in Tick Cell Cultures

Cited by 42 publications

References 25 publications

Tick cell lines: tools for tick and tick-borne disease research

Tick cell lines: tools for tick and tick-borne disease research

Serologic Cross-Reactivity between Anaplasma marginale and Anaplasma phagocytophilum

In vitro cultivation of Anaplasma marginale and A. phagocytophilum in tick cell lines: a review

Contact Info

Product

Resources

About