Cultivation of a novel cold-adapted nitrite oxidizing betaproteobacterium from the Siberian Arctic

Alawi, Mashal; Lipski, André; Sanders, Tina; Pfeiffer, Eva‐Maria; Spieck, Eva

doi:10.1038/ismej.2007.34

Cited by 199 publications

(212 citation statements)

References 31 publications

(40 reference statements)

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“…From the end of the nineteenth century, researchers have continued to investigate the diversity of AOB and NOB, and at present, at least 31 different species of AOB and four genera with eight described species of NOB are widespread in different ecosystems (Alawi et al 2007;Koops et al 1991;Koops and Pommerening-Röser 2001). Generation and maintenance of a nitritation reaction requires that nitrite-oxidizing bacteria are removed from the biomass in biological treatment systems (van Dongen et al 2001).…”

Section: Role Of Nitrite Formationmentioning

confidence: 99%

Potential roles of anaerobic ammonium and methane oxidation in the nitrogen cycle of wetland ecosystems

Zhu

Jetten

Kuschk

et al. 2010

Appl Microbiol Biotechnol

166

108

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Anaerobic ammonium oxidation (anammox) and anaerobic methane oxidation (ANME coupled to denitrification) with nitrite as electron acceptor are two of the most recent discoveries in the microbial nitrogen cycle. Currently the anammox process has been relatively well investigated in a number of natural and man-made ecosystems, while ANME coupled to denitrification has only been observed in a limited number of freshwater ecosystems. The ubiquitous presence of anammox bacteria in marine ecosystems has changed our knowledge of the global nitrogen cycle. Up to 50% of N 2 production in marine sediments and oxygendepleted zones may be attributed to anammox bacteria. However, there are only few indications of anammox in natural and constructed freshwater wetlands. In this paper, the potential role of anammox and denitrifying methanotrophic bacteria in natural and artificial wetlands is discussed in relation to global warming. The focus of the review is to explore and analyze if suitable environmental conditions exist for anammox and denitrifying methanotrophic bacteria in nitrogen-rich freshwater wetlands.

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Section: Role Of Nitrite Formationmentioning

confidence: 99%

Potential roles of anaerobic ammonium and methane oxidation in the nitrogen cycle of wetland ecosystems

Zhu

Jetten

Kuschk

et al. 2010

Appl Microbiol Biotechnol

166

108

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“…After 3 weeks of incubation with 13 C-bicarbonate, sulfur-oxidizing bacteria were still present, including Thiobacillus (four clones), Thiovirga (one clone). Four 16S rRNA clones were associated with the ammonia-oxidizing bacterium Nitrosomonas and 13 clones showed high identity to the 16S rRNA from Candidatus 'Nitrotoga arctica', a novel nitrite-oxidizing bacterium isolated from the Siberian Arctic (Alawi et al, 2007).…”

Section: Diversity Of Soxb and Amoa Genesmentioning

confidence: 99%

Life without light: microbial diversity and evidence of sulfur- and ammonium-based chemolithotrophy in Movile Cave

et al. 2009

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Microbial diversity in Movile Cave (Romania) was studied using bacterial and archaeal 16S rRNA gene sequence and functional gene analyses, including ribulose-1,5-bisphosphate carboxylase/ oxygenase (RuBisCO), soxB (sulfate thioesterase/thiohydrolase) and amoA (ammonia monooxygenase). Sulfur oxidizers from both Gammaproteobacteria and Betaproteobacteria were detected in 16S rRNA, soxB and RuBisCO gene libraries. DNA-based stable-isotope probing analyses using 13 C-bicarbonate showed that Thiobacillus spp. were most active in assimilating CO 2 and also implied that ammonia and nitrite oxidizers were active during incubations. Nitrosomonas spp. were detected in both 16S rRNA and amoA gene libraries from the 'heavy' DNA and sequences related to nitriteoxidizing bacteria Nitrospira and Candidatus 'Nitrotoga' were also detected in the 'heavy' DNA, which suggests that ammonia/nitrite oxidation may be another major primary production process in this unique ecosystem. A significant number of sequences associated with known methylotrophs from the Betaproteobacteria were obtained, including Methylotenera, Methylophilus and Methylovorus, supporting the view that cycling of one-carbon compounds may be an important process within Movile Cave. Other sequences detected in the bacterial 16S rRNA clone library included Verrucomicrobia, Firmicutes, Bacteroidetes, alphaproteobacterial Rhodobacterales and gammaproteobacterial Xanthomonadales. Archaeal 16S rRNA sequences retrieved were restricted within two groups, namely the Deep-sea Hydrothermal Vent Euryarchaeota group and the Miscellaneous Crenarchaeotic group. No sequences related to known sulfur-oxidizing archaea, ammonia-oxidizing archaea, methanogens or anaerobic methane-oxidizing archaea were detected in this clone library. The results provided molecular biological evidence to support the hypothesis that Movile Cave is driven by chemolithoautotrophy, mainly through sulfur oxidation by sulfur-oxidizing bacteria and reveal that ammonia-and nitrite-oxidizing bacteria may also be major primary producers in Movile Cave.

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“…Five genera of NOB have been identified so far: Nitrospira, Nitrospina, Nitrococcus, Nitrobacter and the newly discovered single-species genus Nitrotoga (Alawi et al, 2007). All genera except for Nitrotoga have been detected in marine environments, although Nitrobacter is generally not abundant in oceanic settings (Koops and PommereningRoser, 2001).…”

Section: Introductionmentioning

confidence: 99%

Nitrite oxidation in the Namibian oxygen minimum zone

et al. 2011

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Nitrite oxidation is the second step of nitrification. It is the primary source of oceanic nitrate, the predominant form of bioavailable nitrogen in the ocean. Despite its obvious importance, nitrite oxidation has rarely been investigated in marine settings. We determined nitrite oxidation rates directly in 15 N-incubation experiments and compared the rates with those of nitrate reduction to nitrite, ammonia oxidation, anammox, denitrification, as well as dissimilatory nitrate/nitrite reduction to ammonium in the Namibian oxygen minimum zone (OMZ). Nitrite oxidation (p372 nM NO 2 À d À1 ) was detected throughout the OMZ even when in situ oxygen concentrations were low to non-detectable. Nitrite oxidation rates often exceeded ammonia oxidation rates, whereas nitrate reduction served as an alternative and significant source of nitrite. Nitrite oxidation and anammox co-occurred in these oxygen-deficient waters, suggesting that nitrite-oxidizing bacteria (NOB) likely compete with anammox bacteria for nitrite when substrate availability became low. Among all of the known NOB genera targeted via catalyzed reporter deposition fluorescence in situ hybridization, only Nitrospina and Nitrococcus were detectable in the Namibian OMZ samples investigated. These NOB were abundant throughout the OMZ and contributed up to B9% of total microbial community. Our combined results reveal that a considerable fraction of the recently recycled nitrogen or reduced NO 3 À was re-oxidized back to NO 3 À via nitrite oxidation, instead of being lost from the system through the anammox or denitrification pathways.

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Cultivation of a novel cold-adapted nitrite oxidizing betaproteobacterium from the Siberian Arctic

Cited by 199 publications

References 31 publications

Potential roles of anaerobic ammonium and methane oxidation in the nitrogen cycle of wetland ecosystems

Potential roles of anaerobic ammonium and methane oxidation in the nitrogen cycle of wetland ecosystems

Life without light: microbial diversity and evidence of sulfur- and ammonium-based chemolithotrophy in Movile Cave

Nitrite oxidation in the Namibian oxygen minimum zone

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