Cultivation in industrially relevant conditions has a strong influence on biological properties and performances of Nannochloropsis gaditana genetically modified strains

Perin, Giorgio; Simionato, Diana; Bellan, Alessandra; Riggio, Vincenzo Andrea; Occhipinti, Andrea; Maffei, Massimo E.; Morosinotto, Tomas

doi:10.1016/j.algal.2017.10.013

Cited by 21 publications

(22 citation statements)

References 54 publications

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“…The contribution of linear (LEF) and cyclic electron flow (CEF) pathways to the total electron transport was also quantified using specific inhibitors of PSII and cytochrome b 6 f, respectively ( Figure 6B). Under all conditions, CEF was barely detectable, and LEF was responsible for total electron transport activity, confirming earlier results in Nannochloropsis (Perin et al, 2017b;Simionato et al, 2013).…”

Section: Effect Of Fluctuating Light On Psi and Photosynthetic Electrsupporting

confidence: 90%

Photosynthesis Regulation in Response to Fluctuating Light in the Secondary Endosymbiont Alga Nannochloropsis gaditana

Bellan

Bucci

Perin

et al. 2019

Plant and Cell Physiology

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In nature, photosynthetic organisms are exposed to highly dynamic environmental conditions where the excitation energy and electron flow in the photosynthetic apparatus need to be continuously modulated. Fluctuations in incident light are particularly challenging because they drive oversaturation of photosynthesis with consequent oxidative stress and photoinhibition. Plants and algae have evolved several mechanisms to modulate their photosynthetic machinery to cope with light dynamics, such as thermal dissipation of excited chlorophyll states (non-photochemical quenching, NPQ) and regulation of electron transport. The regulatory mechanisms involved in the response to light dynamics have adapted during evolution, and exploring biodiversity is a valuable strategy for expanding our understanding of their biological roles. In this work, we investigated the response to fluctuating light in Nannochloropsis gaditana, a eukaryotic microalga of the phylum Heterokonta originating from a secondary endosymbiotic event. Nannochloropsis gaditana is negatively affected by light fluctuations, leading to large reductions in growth and photosynthetic electron transport. Exposure to light fluctuations specifically damages photosystem I, likely because of the ineffective regulation of electron transport in this species. The role of NPQ, also assessed using a mutant strain specifically depleted of this response, was instead found to be minor, especially in responding to the fastest light fluctuations.

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Section: Effect Of Fluctuating Light On Psi and Photosynthetic Electrsupporting

confidence: 90%

Photosynthesis Regulation in Response to Fluctuating Light in the Secondary Endosymbiont Alga Nannochloropsis gaditana

Bellan

Bucci

Perin

et al. 2019

Plant and Cell Physiology

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“…can accumulate more than 40% of lipids [ 49 ] and, its content is strongly dependent on growth conditions. In particular, high light intensity can induce a consistent increase of the production of total lipid and triacylglycerols (TAGs) [ 73 , 74 ]. The photoperiod can be also among the factors that affect lipid production, which decreases by up to 25.6% w / w on a dry basis when the photoperiod is 24 h and increases by up to 30% when the photoperiod is 18 h light: 6 h dark cycle [ 72 ].…”

Section: Resultsmentioning

confidence: 99%

Microalgae Characterization for Consolidated and New Application in Human Food, Animal Feed and Nutraceuticals

Molino

Iovine

Casella

et al. 2018

IJERPH

173

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The exploration of new food sources and natural products is the result of the increase in world population as well as the need for a healthier diet; in this context, microalgae are undoubtedly an interesting solution. With the intent to enhance their value in new commercial applications, this paper aims to characterize microalgae that have already been recognized as safe or authorized as additives for humans and animals (Chlorella vulgaris, Arthrospira platensis, Haematococcus pluvialis, Dunaliella salina) as well as those that have not yet been marketed (Scenedesmus almeriensis and Nannocholoropsis sp.). In this scope, the content of proteins, carbohydrates, lipids, total dietary fiber, humidity, ash, and carotenoids has been measured via standard methods. In addition, individual carotenoids (beta-carotene, astaxanthin, and lutein) as well as individual saturated, monounsaturated, and polyunsaturated fatty acids have been identified and quantified chromatographically. The results confirm the prerogative of some species to produce certain products such as carotenoids, polyunsaturated fatty acids, and proteins, but also show how their cellular content is rich and diverse. H. pluvialis green and red phases, and Nannochloropsis sp., in addition to producing astaxanthin and omega-3, contain about 25–33% w/w proteins on a dry basis. D. salina is rich in beta-carotene (3.45% w/w on a dry basis), S. Almeriensis is a source of lutein (0.30% w/w on a dry basis), and the C. vulgaris species is a protein-based microalgae (45% w/w on a dry basis). All, however, can also produce important fatty acids such as palmitic acid, γ-linolenic acid, and oleic acid. Considering their varied composition, these microalgae can find applications in multiple sectors. This is true for microalgae already on the market as well as for promising new sources of bioproducts such as S. almeriensis and Nannochloropsis sp.

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“…Recent scientific efforts have demonstrated enhanced microalgae PBCE [64], albeit only under optimal conditions without consideration for complex dynamic changes in the natural environment. The importance of preparing for the intended cultivation environment is in fact often underestimated [64] and scaling-up trials are also often compromised by contamination [65] and genetic instability of the engineered strains [66], thereby compromising the robustness of the production process [64,67]. Recent advancements illustrate the power of creative synthetic biology, by providing potentially crucial solutions to some of these scale-up problems.…”

Section: Directions From Here 61 Strategies For Further Improvementmentioning

confidence: 99%

Economic feasibility and long-term sustainability criteria on the path to enable a transition from fossil fuels to biofuels

Perin

Jones

2019

Current Opinion in Biotechnology

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Cultivation in industrially relevant conditions has a strong influence on biological properties and performances of Nannochloropsis gaditana genetically modified strains

Cited by 21 publications

References 54 publications

Photosynthesis Regulation in Response to Fluctuating Light in the Secondary Endosymbiont Alga Nannochloropsis gaditana

Photosynthesis Regulation in Response to Fluctuating Light in the Secondary Endosymbiont Alga Nannochloropsis gaditana

Microalgae Characterization for Consolidated and New Application in Human Food, Animal Feed and Nutraceuticals

Economic feasibility and long-term sustainability criteria on the path to enable a transition from fossil fuels to biofuels

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