Cultivation, Growth Media, Division Rates and Applications of Dunaliella Species

Rao, DV Subba

doi:10.1201/b10300-4

Cited by 7 publications

(7 citation statements)

References 135 publications

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“…The increased temperature condition resulted in reduced control algal growth rates and cell density at 96 h, but increased cellular lipid and chlorophyll concentrations. The optimum temperature for growth of D. tertiolecta is 20°C (Subba Rao,2009). Increasing exposure salinity did not significantly alter any of the test endpoints.…”

Section: Discussionmentioning

confidence: 99%

“…When phytoplankton growth is limited, as D. tertiolecta was under the increased temperature conditions, high energy cellular storage products may be accumulated (Subba Rao,2009). In this study, D. tertiolecta showed greater per/cell concentrations of chlorophyll and lipids under temperature stress.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, D. tertiolecta showed greater per/cell concentrations of chlorophyll and lipids under temperature stress. It should be noted that some green algae preferentially store starch, rather than lipids, and some can store either, under different conditions (Subba Rao,2009). In this study, cellular starch content was the only endpoint not affected by exposure condition with control algae.…”

Section: Discussionmentioning

confidence: 99%

“…It is possible that at higher temperatures and salinities there is an increase in the rate of chemical diffusion across the cell membrane, resulting in greater herbicide toxicity and greater starch content. D.tertiolecta lacks a rigid cell wall (Subba Rao,2009), and the Dunaliella cell membrane is known to shrink and then regain size as internal volume goes through osmoregulatory changes (Ben‐Amotz and Avron,1973). Membrane structure and fluidity is also known to change in response to temperature, which may affect permeability (Quinn,1988).…”

Section: Discussionmentioning

confidence: 99%

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Influence of increasing temperature and salinity on herbicide toxicity in estuarine phytoplankton

DeLorenzo

Danese

Baird

2011

Environmental Toxicology

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Ecological risk assessments are, in part, based on results of toxicity tests conducted under standard exposure conditions. Global climate change will have a wide range of effects on estuarine habitats, including potentially increasing water temperature and salinity, which may alter the risk assessment of estuarine pollutants. We examined the effects of increasing temperature and salinity on the toxicity of common herbicides (irgarol, diuron, atrazine, and ametryn) to the phytoplankton species Dunaliella tertiolecta. Static 96-h algal bioassays were conducted for each herbicide under four exposure scenarios: standard temperature and salinity (25°C, 20 ppt), standard temperature and elevated salinity (25°C, 40 ppt), elevated temperature and standard salinity (35°C, 20 ppt), and elevated temperature and elevated salinity (35°C, 40 ppt). The endpoints assessed were algal cell density at 96 h, growth rate, chlorophyll a content, lipid content, and starch content. Increasing exposure temperature reduced growth rate and 96-h cell density but increased the cellular chlorophyll and lipid concentrations of the control algae. Exposure condition did not alter starch content of control algae. Herbicides were found to decrease growth rate, 96 h cell density, and cellular chlorophyll and lipid concentrations, while starch concentrations increased with herbicide exposure. Herbicide effects under standard test conditions were then compared with those observed under elevated temperature and salinity. Herbicide effects on growth rate, cell density, and starch content were more pronounced under elevated salinity and temperature conditions. To encompass the natural variability in estuarine temperature and salinity, and to account for future changes in climate, toxicity tests should be conducted under a wider range of environmental conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Influence of increasing temperature and salinity on herbicide toxicity in estuarine phytoplankton

DeLorenzo

Danese

Baird

2011

Environmental Toxicology

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“…São organismos que toleram condições extremas de temperatura, salinidade, pH e iluminação (Subba Rao, 2009) e possuem um ciclo de vida curto, envolvendo algumas gerações durante o tempo de duração do experimento, que permite a avaliação de efeitos sub-letais (Anastácio et al, 2000) em diversas condições.…”

Section: Utilização De Dunaliella Tertiolecta Em Ensaios Ecotoxicológunclassified

Sensibilidade da microalga marinha Dunaliella tertiolecta BUTCHER a diferentes substâncias químicas utilizadas como referência em ensaios ecotoxicológicos

Minei¹

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À profª Ceci, sempre muito paciente e atenciosa, principalmente pelo convívio e orientação. Obrigada pela oportunidade e por me apresentar à Ecotoxicologia. À Marcia, técnica do laboratório, que participou de todas as etapas, desde o estágio até a conclusão deste trabalho de mestrado, contribuindo com muitas informações e sugestões. À Dra. Sônia Gianesella pelo fornecimento da microalga Dunaliella tertiolecta, utilizada neste trabalho e disponibilização de equipamentos do Laboratório de Microrganismos Marinhos. À Dra. Flávia Saldanha-Corrêa e aos técnicos Tomás Silva e especialmente à Marta Stephan, por tornarem possível a realização deste trabalho. Ao Dr. David pelos esclarecimentos sobre a metodologia. À técnica Maysa Pompeu, pelo auxílio com a microscopia de contraste de fase. À bióloga Paula Nishimura, do Instituto de Biociências pela ajuda com a montagem das lâminas semi-permanentes. Aos biólogos da Aplysia Katia, Felipe e Karina, por sanarem muitas dúvidas estatísticas. Aos membros da Comissão de Estudo Especial de Análises Ecotoxicológicas da ABNT pelas discussões muito produtivas durante a revisão do protocolo de ensaios com microalgas.

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The beauty and the yeast: can the microalgae Dunaliella form a borderline lichen with Hortaea werneckii?

et al. 2020

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Lichenized fungi usually develop complex, stratified morphologies through an intricately balanced living together with their algal partners, but several species are known to form only more or less loose associations with algae. These borderline lichens are still little explored although they could inform us about early stages of lichen evolution. We studied the association of the extremely halotolerant fungus Hortaea werneckii with the alga Dunaliella atacamensis, discovered in a cave in the Atacama Desert (Chile), and with D. salina, common inhabitant of saltern brines. D. atacamensis forms small colonies, in which cells of H. werneckii can be frequently observed, while such interaction has not been observed with D. salina. As symbiotic interactions between Dunaliella and Hortaea have not been reported, we performed a series of co-cultivation experiments to inspect whether these species could interact and develop more distinct lichen-like symbiotic structures. We set up co-cultures between axenic strains of Hortaea werneckii (isolated both from Mediterranean salterns and from the Atacama cave) and isolates of D. atacamensis (from the Atacama cave) and D. salina (isolated from Mediterranean salterns). Although we used different growth media and cultivation approaches, bright field and SEM microscopy analyses did not indicate any mutual effects in these experiments. We discuss the implications for fungal algal interactions along the transition from algal exploiters to lichen symbioses.

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Cultivation, Growth Media, Division Rates and Applications of Dunaliella Species

Cited by 7 publications

References 135 publications

Influence of increasing temperature and salinity on herbicide toxicity in estuarine phytoplankton

Influence of increasing temperature and salinity on herbicide toxicity in estuarine phytoplankton

Sensibilidade da microalga marinha Dunaliella tertiolecta BUTCHER a diferentes substâncias químicas utilizadas como referência em ensaios ecotoxicológicos

The beauty and the yeast: can the microalgae Dunaliella form a borderline lichen with Hortaea werneckii?

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