Cultivation and Visualization of Mycobacteria on Molecular Filter Membranes

Abstract

The molecular filter membrane (MF) was developed for the recovery of small numbers of bacterial contminants in water and air (Goets,

Mycobacterium Fortuitum Cruz From the Tropical Fish Hyphessobrycon Innesi

“…Two methods were used for this determination: (a) The tube technique described by Dubos and Middlebrook (1948) was employed using 5-day-old cultures from heart infusion agar. (b) Similar cultures were used in the molecular filter membrane procedure as outlined by Wayne (1955).…”

Use of membrane filters for measurement of mycobactericidal activity of alkaline glutaraldehyde solution

“…In an attempt to obtain a more quantitative procedure, mycobacteria were attached to a Millipore membrane surface before being exposed to the glutaraldehyde solution. The membrane was then cultured on agar medium in a manner similar to that used in the direct recovery and enumeration of mycobacteria present in air, water, and clinical samples (7,8,10). This allowed the direct enumeration of the surviving bacilli on the membrane surface, providing a direct demonstration of sterility after the bacterial population had been exposed to the standard disinfectant solution.…”