Cultivation and Immortalization of Human B‐Cells Producing a Human Monoclonal IgM Antibody Binding to MDA‐LDL: Further Evidence for Formation of Atherogenic MDA‐LDL Adducts in Humans <i>In Vivo</i>

Tatzber, Franz; Pursch, Edith; Resch, Ulrike; Pfragner, Roswitha; Holasek, Sandra; Lindschinger, Meinrad; Cvirn, Gerhard; Wonisch, Willibald

doi:10.1155/2017/6047142

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…MDA-LDL IgM was measured with the MDA-LDL-IgM ELISA from Omnignostica Ltd. (Höflein/D., Austria), which is standardized on a human monoclonal antibody as described previously [ 17 ]. Serum myeloperoxidase (MPO) concentrations were measured with the MPO enzyme-linked immunosorbent assay (ELISA) Kit (Immundiagnostik AG, Bensheim, Germany) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Work Intensity, Low‐Grade Inflammation, and Oxidative Status: A Comparison between Office and Slaughterhouse Workers

Zelzer

Tatzber

Herrmann

et al. 2018

Oxidative Medicine and Cellular Longevity

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Limited knowledge exists about the impact of physical workload on oxidative stress in different occupational categories. Thus, we aimed to investigate the oxidative and inflammatory status in employees with different physical workloads. We enrolled a total of 79 male subjects, 27 office workers (mean age 38.8 ± 9.1 years) and 52 heavy workers, in a slaughterhouse (mean age 40.8 ± 8.2 years). Fasting blood was drawn from an antecubital vein in the morning of the midweek before an 8-hour or 12-hour work shift. The antioxidative capacity was assessed measuring total antioxidant capacity (TAC), uric acid, total polyphenols (PPm), and endogenous peroxidase activity (EPA). Total peroxides (TOC), malondialdehyde (MDA), and myeloperoxidase (MPO) were analyzed as prooxidative biomarkers, and an oxidative stress index (OSI) was calculated. In addition, hsCRP, interleukin-6 (IL-6), MDA-LDL IgM antibodies, galectin-3, adrenocorticotropic hormone (ACTH), and the brain-derived neurotrophic factor (BDNF) were measured as biomarkers of chronic systemic inflammation and emotional stress. TOC (p = 0.032), TAC (p < 0.001), ACTH (p < 0.001), OSI (p = 0.011), and hsCRP (p = 0.019) were significantly increased in the heavy workers group, while EPA, BDNF (p < 0.001), and polyphenols (p = 0.004) were significantly higher in office workers. Comparison between 8 and 12 h shifts showed a worse psychological condition in heavy workers with increased levels for hsCRP (p = 0.001) and reduced concentration of BDNF (p = 0.012) compared to office workers. Oxidative stress and inflammation are induced in heavy workers and are particularly pronounced during long working hours, that is, 12-hour versus 8-hour shifts.

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Section: Methodsmentioning

confidence: 99%

Work Intensity, Low‐Grade Inflammation, and Oxidative Status: A Comparison between Office and Slaughterhouse Workers

Zelzer

Tatzber

Herrmann

et al. 2018

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

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“…Therefore, even very efficient antisera are only available for a limited period of time. Of course, immunization of mammals can be carried out to obtain immunoglobulin-producing cells, which could then be immortalized by fusion with, e.g., mouse myeloma cells [ 20 ]. Such immunoglobulins are so-called monoclonal antibodies (MCA) and are at least theoretically available in unlimited amounts.…”

Section: Strategies and Effects Of Passive Immunization In Times Of P...mentioning

confidence: 99%

“…Östberg and Pursch published such a technique in 1983 [ 22 ]. In addition, for conclusiveness, an hMCA against malondialdehyde-modified low-density lipoprotein was previously published [ 20 ].…”

Section: Strategies and Effects Of Passive Immunization In Times Of P...mentioning

confidence: 99%

Thinking beyond Vaccination: Promising Add-On Strategies to Active Immunization and Vaccination in Pandemics—A Mini-Review

Tatzber¹,

Wonisch²,

Resch

et al. 2023

Viruses

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There is little doubt that final victories over pandemics, such as COVID-19, are attributed to herd immunity, either through post-disease convalescence or active immunization of a high percentage of the world’s population with vaccines, which demonstrate protection from infection and transmission and are available in large quantities at reasonable prices. However, it is assumable that humans with immune defects or immune suppression, e.g., as a consequence of allograft transplantation, cannot be immunized actively nor produce sufficient immune responses to prevent SARS-CoV-2 infections. These subjects desperately need other strategies, such as sophisticated protection measures and passive immunization. Hypertonic salt solutions attack vulnerable core areas of viruses; i.e., salt denatures surface proteins and thus prohibits virus penetration of somatic cells. It has to be ensured that somatic proteins are not affected by denaturation regarding this unspecific virus protection. Impregnating filtering facepieces with hypertonic salt solutions is a straightforward way to inactivate viruses and other potential pathogens. As a result of the contact of salt crystals on the filtering facepiece, these pathogens become denatured and inactivated almost quantitatively. Such a strategy could be easily applied to fight against the COVID-19 pandemic and other ones that may occur in the future. Another possible tool to fight the COVID-19 pandemic is passive immunization with antibodies against SARS-CoV-2, preferably from human origin. Such antibodies can be harvested from human patients’ sera who have successfully survived their SARS-CoV-2 infection. The disadvantage of a rapid decrease in the immunoglobulin titer after the infection ends can be overcome by immortalizing antibody-producing B cells via fusion with, e.g., mouse myeloma cells. The resulting monoclonal antibodies are then of human origin and available in, at least theoretically, unlimited amounts. Finally, dry blood spots are a valuable tool for surveilling a population’s immunity. The add-on strategies were selected as examples for immediate, medium and long-term assistance and therefore did not raise any claim to completeness.

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Thinking beyond Vaccination: Promising Add-On Strategies to Active Immunization and Vaccination in Pandemics - a Mini-Review

Tatzber¹,

Wonisch²,

Resch³

et al. 2023

Preprint

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There is little doubt that final victories over pandemics, such as COVID-19, are attributed to herd immunity, either through post-disease convalescence or active immunization of a high percentage of the world's population with vaccines, demonstrating protection from infection and transmission, being available in large quantities and at reasonable prices. However, it is assumable that humans with immune defects or immune suppression, e.g., as a consequence of allograft transplantation, cannot be immunized actively nor produce sufficient immune responses to prevent SARS-CoV-2 infections. These subjects desperately need other strategies, such as sophisticated protection measures and active immunization. Hypertonic salt solutions attack vulnerable core areas of viruses, i.e., salt denatures surface proteins and thus prohibits virus penetration of somatic cells. It has to be ensured that somatic proteins are not affected by denaturation regarding this unspecific virus protection. Impregnating filtering facepieces with hypertonic salt solutions is a straightforward way to inactivate viruses and other potential pathogens. As a result of the contact of salt crystals on the filtering facepiece, these pathogens become denatured and inactivated almost quantitatively. Such a strategy could be easily applied to fight against the COVID-19 pandemic and other ones that may occur in the future. Another possible tool to fight the COVID-19 pandemic is passive immunization with antibodies against SARS-CoV-2, preferably from human origin. Such antibodies can be harvested from patients´ human sera, which have successfully survived their SARS-CoV-2 infection. The disadvantage of a rapid decrease of the immunoglobulin titer after infection ends can be overcome by immortalizing antibody-producing B-cells via fusion with, e.g., mouse myeloma cells. The resulting monoclonal antibodies are then of human origin and available in, at least theoretically, unlimited amounts. Finally, dry blood spots are a valuable tool for surveilling the population´s immunity.

show abstract

Cultivation and Immortalization of Human B‐Cells Producing a Human Monoclonal IgM Antibody Binding to MDA‐LDL: Further Evidence for Formation of Atherogenic MDA‐LDL Adducts in Humans In Vivo

Cited by 3 publications

References 27 publications

Work Intensity, Low‐Grade Inflammation, and Oxidative Status: A Comparison between Office and Slaughterhouse Workers

Work Intensity, Low‐Grade Inflammation, and Oxidative Status: A Comparison between Office and Slaughterhouse Workers

Thinking beyond Vaccination: Promising Add-On Strategies to Active Immunization and Vaccination in Pandemics—A Mini-Review

Thinking beyond Vaccination: Promising Add-On Strategies to Active Immunization and Vaccination in Pandemics - a Mini-Review

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