Cultivation and biochemical characterization of heterotrophic bacteria associated with phytoplankton bloom in the Amundsen sea polynya, Antarctica

Choi, Seon-Bin; Kim, Jong-Geol; Jung, Man‐Young; Kim, So-Jeong; Min, Ui-Gi; Si, Ok-Ja; Park, Soo-Je; Hwang, Chung Yeon; Park, Jisoo; Lee, Sanghoon; Rhee, Sung‐Keun

doi:10.1016/j.dsr2.2015.04.027

Cited by 10 publications

(8 citation statements)

References 44 publications

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“…3 ). The concomitant occurrence of these three species is not surprising since they are among the most important ones during phytoplankton bloom as reported by Choi et al (2016) in an Antarctic polynya. Recently, a Sulfitobacter species was isolated from the toxic marine diatom Pseudo-nitzschia multiseries ( Hong et al, 2015 ).…”

Section: Discussionsupporting

confidence: 56%

Mussel biofiltration effects on attached bacteria and unicellular eukaryotes in fish-rearing seawater

Voudanta

Kormas

Monchy

et al. 2016

PeerJ

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Mussel biofiltration is a widely used approach for the mitigation of aquaculture water. In this study, we investigated the effect of mussel biofiltration on the communities of particle-associated bacteria and unicellular eukaryotes in a sea bass aquaculture in southern North Sea. We assessed the planktonic community changes before and after biofiltration based on the diversity of the 16S and 18S rRNA genes by using next generation sequencing technologies. Although there was no overall reduction in the operational taxonomic units (OTU) numbers between the control (no mussels) and the test (with mussels) tanks, a clear reduction in the relative abundance of the top three most dominant OTUs in every sampling time was observed, ranging between 2–28% and 16–82% for Bacteria and Eukarya, respectively. The bacterial community was dominated by OTUs related to phytoplankton blooms and/or high concentrations of detritus. Among the eukaryotes, several fungal and parasitic groups were found. Their relative abundance in most cases was also reduced from the control to the test tanks; a similar decreasing pattern was also observed for both major higher taxa and functional (trophic) groups. Overall, this study showed the effectiveness of mussel biofiltration on the decrease of microbiota abundance and diversity in seawater fueling fish farms.

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Section: Discussionsupporting

confidence: 56%

Mussel biofiltration effects on attached bacteria and unicellular eukaryotes in fish-rearing seawater

Voudanta

Kormas

Monchy

et al. 2016

PeerJ

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“…Ammonium concentrations are seasonally variable in the WAP with peak concentrations (>3.5 μm) recorded in early autumn (March–May) (Henley et al, 2017 ). Previous studies have established successional patterns between phytoplankton blooms and some specific bacterioplankton members (Abell and Bowman, 2005 ; Obernosterer et al, 2011 ; Kim et al, 2014 ; Choi, 2016 ; Luria et al, 2016 ; Liu et al, 2019 ). In the PF, AS, and AP zones, the generalist Oceanospirillales, Rhodobacterales , and Flavobacteriales dominated the indicator taxa.…”

Section: Discussionmentioning

confidence: 99%

Biogeography of Southern Ocean Active Prokaryotic Communities Over a Large Spatial Scale

Maturana-Martínez

Iriarte

et al. 2022

Front. Microbiol.

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The activity of marine microorganisms depends on community composition, yet, in some oceans, less is known about the environmental and ecological processes that structure their distribution. The objective of this study was to test the effect of geographical distance and environmental parameters on prokaryotic community structure in the Southern Ocean (SO). We described the total (16S rRNA gene) and the active fraction (16S rRNA-based) of surface microbial communities over a ~6,500 km longitudinal transect in the SO. We found that the community composition of the total fraction was different from the active fraction across the zones investigated. In addition, higher α-diversity and stronger species turnover were displayed in the active community compared to the total community. Oceanospirillales, Alteromonadales, Rhodobacterales, and Flavobacteriales dominated the composition of the bacterioplankton communities; however, there were marked differences at the order level. Temperature, salinity, silicic acid, particulate organic nitrogen, and particulate organic carbon correlated with the composition of bacterioplankton communities. A strong distance–decay pattern between closer and distant communities was observed. We hypothesize that it was related to the different oceanic fronts present in the Antarctic Circumpolar Current. Our findings contribute to a better understanding of the complex arrangement that shapes the structure of bacterioplankton communities in the SO.

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“…The obligate psychrophilic nature of Polaribacter spp. among members of Bacteroidetes isolated from Southern Ocean including ASP [58] may contribute to their adaptation to blooms of ASP [59]. The high ANI value and conserved synteny between fosmid clone Ant4D3 [60] and GM1_Ant, which was dominant in all phases of the polynya bloom (see Additional file 3: Figure S9), support the recurring pattern of the most well-adapted clades in the Southern Ocean.…”

Section: Discussionmentioning

confidence: 83%

Genomic and metatranscriptomic analyses of carbon remineralization in an Antarctic polynya

Kim

Lee

et al. 2019

Microbiome

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Background Polynyas in the Southern Ocean are regions of intense primary production, mainly by Phaeocystis antarctica . Carbon fixed by phytoplankton in the water column is transferred to higher trophic levels, and finally, to the deep ocean. However, in the Amundsen Sea, most of this organic carbon does not reach the sediment but is degraded in the water column due to high bacterial heterotrophic activity. Results We reconstructed 12 key bacterial genomes from different phases of bloom and analyzed the expression of genes involved in organic carbon remineralization. A high correlation of gene expression between the peak and decline phases was observed in an individual genome bin-based pairwise comparison of gene expression. Polaribacter belonging to Bacteroidetes was found to be dominant in the peak phase, and its transcriptional activity was high (48.9% of the total mRNA reads). Two dominant Polaribacter bins had the potential to utilize major polymers in P. antarctica , chrysolaminarin and xylan, with a distinct set of glycosyl hydrolases. In the decline phase, Gammaproteobacteria (Ant4D3, SUP05, and SAR92), with the potential to utilize low molecular weight-dissolved organic matter (LMW-DOM) including compatible solutes, was increased. The versatility of Gammaproteobacteria may contribute to their abundance in organic carbon-rich polynya waters, while the SAR11 clade was found to be predominant in the sea ice-covered oligotrophic ocean. SAR92 clade showed transcriptional activity for utilization of both polysaccharides and LMW-DOM; this may account for their abundance both in the peak and decline phases. Ant4D3 clade was dominant in all phases of the polynya bloom, implicating the crucial roles of this clade in LMW-DOM remineralization in the Antarctic polynyas. Conclusions Genomic reconstruction and in situ gene expression analyses revealed the unique metabolic potential of dominant bacteria of the Antarctic polynya at a finer taxonomic level. The information can be used to predict temporal community succession linked to the availability of substrates derived from the P. antarctica bloom. Global warming has resulted in compositional changes in phytoplankton from P. antarctica to diatoms, and thus, repeated parallel studies in various polynyas are required to predict global warming-related changes in carbon remineralization. Electronic supplementary material The online version of this article (10.1186/s40168-019-0643-4) contains supplementary material, which is available to authorized users.

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Cultivation and biochemical characterization of heterotrophic bacteria associated with phytoplankton bloom in the Amundsen sea polynya, Antarctica

Cited by 10 publications

References 44 publications

Mussel biofiltration effects on attached bacteria and unicellular eukaryotes in fish-rearing seawater

Mussel biofiltration effects on attached bacteria and unicellular eukaryotes in fish-rearing seawater

Biogeography of Southern Ocean Active Prokaryotic Communities Over a Large Spatial Scale

Genomic and metatranscriptomic analyses of carbon remineralization in an Antarctic polynya

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