Cultivar variation for imazamox resistance in wheat (Triticum aestivum L.): Insights into enzymatic assays for early selection

Breccia, Gabriela; Picardi, Liliana Amelia; Nestares, G.

doi:10.1016/j.plaphy.2020.03.045

Cited by 4 publications

(4 citation statements)

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“…In wheat, the hexaploid variation for many agronomic traits is modulated by genetic interactions among homeologous genes in the A, B, and D subgenomes (Borrill, Adamski, & Uauy, 2015). In some wheat cultivars, the ahasL gene from the D subgenome appears to have a greater contribution to the enzyme pool (Breccia et al., 2020; Hanson et al., 2006). However, the results of the present study allow deducing that ahasL‐D1 transcript levels did not vary among the three wheat lines evaluated (Figure 1c).…”

Section: Discussionmentioning

confidence: 99%

“…Previous works have demonstrated that the in vivo AHAS assay reflects the resistance at whole‐plant level (Breccia et al., 2020; Rainbolt, Thill, Zemetra, & Shaner, 2005). In the present study, the whole‐plant assay showed that the resistance level of the R2 line was threefold higher than that of the R1 line (Figure 5, Table 4).…”

Section: Discussionmentioning

confidence: 99%

“…Leaf and root tissues were ground in liquid nitrogen and suspended in 5 ml g −1 fresh weight of buffer containing 50 mM N‐(2‐hydroxyethyl)‐piperazine‐N9‐(2‐ethanesulfonic acid), pH 7, 200 mM sodium pyruvate, 20 mM MgCl 2 , 2 mM thiamine pyrophosphate, and 20 μM flavin adenine dinucleotide. The homogenate was centrifuged at 12,000 g for 10 min and immediately used for enzyme activity assays, as previously (Breccia, Picardi, & Nestares, 2020). Data were analyzed by two‐way ANOVA (line and tissue as fixed factors) and means were separated using Tukey's test at a 5% probability level, using the R and agricolae package (de Mendiburu, 2019).…”

Section: Methodsmentioning

confidence: 99%

“…In vivo AHAS activity was determined as previously (Breccia et al, 2020). The experiment was conducted with a completely Crop Science randomized design and three replicates.…”

Section: In Vivo Ahas Dose-response Curvesmentioning

confidence: 99%

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An integrated approach for the characterization of one‐ and two‐gene imazamox‐resistant wheat lines

et al. 2020

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In wheat (Triticum aestivum L.), cultivars resistant to imidazolinone herbicides are an important tool for weed management. The target site of imidazolinones is acetohydroxyacid synthase (AHAS), an enzyme coded by three homeologous genes (ahasL‐A1, ahasL‐B1, and ahasL‐D1). This study aimed to evaluate the effect of the imidazolinone herbicide imazamox on a single‐gene (AhasL‐D1) resistant line (R1) and a two‐gene (AhasL‐B1 and AhasL‐D1) resistant line (R2) at multiple biological levels. These lines showed variation in the transcriptional levels of ahasL homeologs, but no differences in total AHAS activity. The relative contribution of resistant alleles to the ahasL transcript pool accounted for 47% in the R1 line and 77% in the R2 line. The in vitro AHAS inhibition of the R1 line was intermediate between the R2 and the susceptible lines. At an anatomical level, high imazamox concentrations induced alterations in the root tip features of R1 plants but did not affect R2 plants, whereas at in vivo AHAS activity and whole‐plant levels, both resistant lines showed high resistance. The herbicide dose that reduced shoot biomass of resistant lines by 50% was 35‐fold higher than that of the susceptible line. We conclude that homeolog variation allows a higher contribution of resistance genes to the ahasL transcript pool in the R1 and R2 lines. Although both resistant lines showed high whole‐plant resistance, the differential root damage induced by imazamox between wheat lines suggests that two‐gene resistant cultivars could be an effective option to avoid the negative impacts caused by herbicide residues in the soil.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…In vivo AHAS activity was determined as previously (Breccia et al, 2020). The experiment was conducted with a completely Crop Science randomized design and three replicates.…”

Section: In Vivo Ahas Dose-response Curvesmentioning

confidence: 99%

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