Cultivar preference exhibited by two sympatric and genetically distinct populations of the soybean fungal pathogen <i>Phialophora gregata</i> f.sp. <i>sojae</i>

Meng, Xiangqi; Grau, C. R.; Chen, W.

doi:10.1111/j.1365-3059.2005.01162.x

Cited by 8 publications

(9 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results were found in other plant pathogens, e.g. Phialophora gregata (Meng et al ., 2005). The comparison between the two regions is interesting, because, as with P. gregata , the two groups A and B of grapevine powdery mildew are associated with different symptoms and show cultivar preference.…”

Section: Discussionsupporting

confidence: 89%

A single nucleotide polymorphism in the β‐tubulin gene distinguishing two genotypes of Erysiphe necator expressing different symptoms on grapevine

Amrani

Corio‐Costet²

2006

Plant Pathology

View full text Add to dashboard Cite

The biotrophic fungus Erysiphe necator (formerly Uncinula necator), for which two genetic groups have been described in European vineyards, is the causal agent of grapevine powdery mildew. By analysing the pathogen population with respect to polymorphism in the sequence of the β‐tubulin gene, which distinguishes two groups of isolates, a new tool was developed for epidemiological and population studies and tested in the vineyard. As in many ascomycetes, the β‐tubulin gene of E. necator (Entub) includes six introns and seven exons and encodes a 447‐amino‐acid protein. A single nucleotide polymorphism (SNP) in the intron‐3 region of the Entub gene distinguished two genetic groups (A and B). This method was used to examine differences in the ratio of the two groups from a total of 289 grape powdery mildew samples collected at the beginning of the growing season from either flag shoots or leaves with sparse‐spot symptoms in four different vineyards. The SNP in the intron‐3 region of the β‐tubulin gene, similar to SNPs in the CYP51 gene, was associated with genotypes A and B of E. necator and confirmed the existence of two sympatric populations of the pathogen in the French vineyards. Differences in the relative proportions of each group varied with the presence or absence of flag‐shoot symptoms and with the region in which isolates had been collected.

show abstract

Section: Discussionsupporting

confidence: 89%

A single nucleotide polymorphism in the β‐tubulin gene distinguishing two genotypes of Erysiphe necator expressing different symptoms on grapevine

Amrani

Corio‐Costet²

2006

Plant Pathology

View full text Add to dashboard Cite

show abstract

“…In addition, the sensitivity and specificity of recently developed q-PCR assays were used in conjunction with standard methods to study the interaction between soybean and P. gregata f. sp. so- (25), the results of this study suggest that both stem and soil populations of P. gregata f. sp. sojae are influenced by host genetics.…”

Section: Discussionmentioning

confidence: 53%

“…Earlier studies by Chen et al (8), Malvick et al (23), and Meng et al (25) have provided evidence suggesting that P. gregata f. sp. sojae genotypes A and B exhibit cultivar preference.…”

Section: Discussionmentioning

confidence: 97%

“…In general, isolates of genotype A are considered more aggressive than genotype B, causing more severe foliar symptoms on BSR-susceptible cultivars. Studies by Chen et al (8), Malvick et al (23), and Meng et al (25) also suggest that soybean cultivars are preferentially colonized by isolates of genotype A and B. Cultivars classified as resistant to BSR were colo-nized more frequently by P. gregata f. sp. sojae genotype B than genotype A, whereas cultivars susceptible to BSR were colonized more frequently by P. gregata f. sp.…”

mentioning

confidence: 93%

See 1 more Smart Citation

Influence of Monocropping Brown Stem Rot–Resistant and –Susceptible Soybean Accessions on Soil and Stem Populations of Phialophora gregata f. sp. sojae

et al. 2009

Self Cite

View full text Add to dashboard Cite

Brown stem rot (BSR)-resistant and -susceptible soybean accessions were continuously cropped in an area never previously seeded to soybean to study the influence of monocultures on soil and stem populations of Phialophora gregata f. sp. sojae. P. gregata f. sp. sojae population size and genotype composition were determined by dilution plating, isolation of P. gregata f. sp. sojae and standard polymerase chain reaction (PCR), and by quantitative real-time PCR (q-PCR. In general, the sizes of P. gregata f. sp. sojae populations in soil were similar regardless of monoculture. The percentage of P. gregata f. sp. sojae genotype B was greater than A in soil following the monoculture of both BSR-susceptible and -resistant soybean accessions. Following the monoculture of a BSR-resistant accession, the percentage of P. gregata f. sp. sojae genotype B was greater than A. Overall, P. gregata f. sp. sojae populations in stems of a BSR-susceptible accession were greater than those in stems of a BSR-resistant accession. P. gregata f. sp. sojae genotype B was detected more often than A in stems of both resistant and susceptible accessions planted following a BSR-resistant monoculture. P. gregata f. sp. sojae genotype B was also detected more often than A in stems of a BSR-resistant accession planted following a BSR-susceptible monoculture. P. gregata f. sp. sojae genotypes A and B were isolated at similar frequencies from stems of a BSR-susceptible accession planted following a BSR-susceptible monoculture. However, q-PCR results indicate that the percentage of P. gregata f. sp. sojae genotype A was greater than B in stems of a BSR-susceptible accession planted following a BSR-susceptible monoculture. Among BSR-susceptible accessions, those with the soybean cyst nematode (SCN)-resistant cv. Peking in their parentage had the largest populations of P. gregata f. sp. sojae and a greater percentage of P. gregata f. sp. sojae genotype B. Similar results were observed for BSR-resistant accessions derived from SCN-resistant PI 88788.

show abstract

“…Also, the two genotypes displayed cultivar preference (Chen et al. , 2000; Meng et al. , 2005; Malvick & Impullitti, 2007).…”

Section: Discussionmentioning

confidence: 99%

Genotyping Cephalosporium gramineum and development of a marker for molecular diagnosis

Baaj

Kondo

2011

Plant Pathology

View full text Add to dashboard Cite

This study investigated the genetic variation of 40 isolates of Cephalosporium gramineum, the causal agent of cephalosporium stripe disease of wheat, based on variations in internal transcribed spacers (ITS) and intergenic spacers (IGS) of rDNA. Of the isolates, 29 were from Japan and the rest from the USA and Europe. The ITS region was about 600 bp and almost identical among these isolates. In the IGS region (5 kbp), restriction fragment length polymorphism analysis detected four genotypes among the 40 isolates. One representative isolate was selected from each of the four genotypes, and the IGS region was sequenced. Attempts to design a genotype-specific marker based on the size of PCR products amplified with selected primers failed to differentiate among the four genotypes. Alternatively, a species-specific primer set (CGIGS1 and CGIGS2) was developed that annealed within the conserved region, producing a DNA fragment of about 1AE8 kbp. Tests of this primer set on a wide range of other fungi from 11 genera confirmed that it was specific to C. gramineum. This primer set could serve as an effective tool in the molecular diagnosis of C. gramineum and has the potential to assist in a better understanding of the host-pathogen interaction.

show abstract

Cultivar preference exhibited by two sympatric and genetically distinct populations of the soybean fungal pathogen Phialophora gregata f.sp. sojae

Cited by 8 publications

References 33 publications

A single nucleotide polymorphism in the β‐tubulin gene distinguishing two genotypes of Erysiphe necator expressing different symptoms on grapevine

A single nucleotide polymorphism in the β‐tubulin gene distinguishing two genotypes of Erysiphe necator expressing different symptoms on grapevine

Influence of Monocropping Brown Stem Rot–Resistant and –Susceptible Soybean Accessions on Soil and Stem Populations of Phialophora gregata f. sp. sojae

Genotyping Cephalosporium gramineum and development of a marker for molecular diagnosis

Contact Info

Product

Resources

About