2013
DOI: 10.17221/452/2012-cjfs
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Cultivar influence on total polyphenol and rutin contents and total antioxidant capacity in buckwheat, amaranth, and quinoa seeds

Abstract: Vollmannová A., Margitanová E., Tóth T., Timoracká M., Urminská D., Bojňanská T., Čičová I. (2013): Cultivar influence on total polyphenol and rutin contents and total antioxidant capacity in buckwheat, amaranth, and quinoa seeds. Czech J. Food Sci., 31: 589-590.Five cultivars from each of the three types of pseudocereals, i.e. buckwheat, amaranth, and quinoa, were studied for total polyphenol and rutin contents as well as total antioxidant capacity of seeds. A spectrophotometric method was used for the deter… Show more

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Cited by 48 publications
(28 citation statements)
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“…a. The level observed for raw quinoa extract was 266.0 ± 15.9 μmol TE/100 g DW, which was comparable to those reported by other authors using the same method in quinoa seeds (Alvarez‐Jubete et al ., ; Dini et al ., ; Vollmannová et al ., ). Roasting process at 100 °C or 130 °C did not change the radical scavenging activity of quinoa extracts significantly ( P > 0.05) compared to unroasted sample; even a decrease was observed in the samples roasted up to 30 min for both temperatures.…”
Section: Resultsmentioning
confidence: 97%
“…a. The level observed for raw quinoa extract was 266.0 ± 15.9 μmol TE/100 g DW, which was comparable to those reported by other authors using the same method in quinoa seeds (Alvarez‐Jubete et al ., ; Dini et al ., ; Vollmannová et al ., ). Roasting process at 100 °C or 130 °C did not change the radical scavenging activity of quinoa extracts significantly ( P > 0.05) compared to unroasted sample; even a decrease was observed in the samples roasted up to 30 min for both temperatures.…”
Section: Resultsmentioning
confidence: 97%
“…The antioxidant capacity of the buckwheat extracts was determined using the DPPH (2.2-diphenyl-1-picrylhydrazyl) free radical scavenging method as described by Vollmannova et al (2013) with a minor modification. An aliquot (0.1 ml) of the sample extract (or pure methanol as blank) was mixed with 3.9 ml of 25 mM methanolic solution of the DPPH radical and vortexed (WiseMix VM-10, Daihan, Korea) for 15-30 s. The reaction was allowed to proceed in the dark at room temperature for 30 min, and the absorbance at 515 nm was then measured with a UV⁄ VIS spectrophotometer (Shimadzu 1800, Kyoto, Japan).…”
Section: Chemical Analysismentioning
confidence: 99%
“…Trolox was used as a reference standard, and antioxidant capacity was expressed as µmol Trolox equivalents (TE) per g dry weight of sample according to the calibration curve (y = 116.5x + 10.508, R 2 = 0.9917). The total phenolic content of the extracts was determined using the Folin-Ciocalteu method as described by Vollmannova et al (2013) with a minor modification. Diluted buckwheat extracts (1 ml) were mixed with 2.5 ml Folin-Ciocalteu reagent and 5 ml deionized H 2 O in 50-ml volumetric flasks and vortexed (WiseMix VM-10, Daihan, Korea) for 15 s. After the 3-min incubation, 7.5 ml of 20% Na 2 CO 3 was added and the mixture was diluted to 50 ml with deionized H 2 O.…”
Section: Chemical Analysismentioning
confidence: 99%
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“…Moreover, the phytochemical composition of cereal crops mainly depends quantitatively and qualitatively on their genotypes and environmental factors that affect growth [16,17]. Although many studies have measured the total phenolic contents and antioxidant capacity in some buckwheat varieties [12,18], information remains limited regarding the characterization and contents of free phenolic (FP) and bound phenolic (BP) fractions of different buckwheat varieties and their corresponding in vitro biological activities (especially anti-diabetic effects). Furthermore, the contributions of the total phenolic contents (TPC), total flavonoid contents (TFC), and the content of individual phenolic on their bio-activities has not been clearly investigated.…”
Section: Introductionmentioning
confidence: 99%