Cultivar identification in dry hop cones and pellets using microsatellite loci

Korbecka-Glinka, Grażyna; Skomra, U.; Olszak-Przybyś, Hanna

doi:10.1007/s00217-016-2715-z

Cited by 7 publications

(18 citation statements)

References 27 publications

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“…Hop is a labor-intensive crop for growers and a relatively expensive raw material for brewers. The specific taste and aroma profiles of beers depend on the hop variety or combination of varieties used and the character of the addition during the brewing process. − Therefore, quality control of a given hop batch supplied to the brewer is essential, especially on varietal origin. , Distinction between varieties can be based on plant morphological characteristics and the distinctive profile of biochemical components such as hop bitter acids, essential oils, or polyphenols. − However, the varietal origin of processed hop products used in brewing practice can hardly be determined unequivocally by observation of their external appearance, and in current brewing practice, determination of varietal origin of hop batches is mostly based on biochemical markers such as the content of hop bitter acids and essential oils. ,, This is, however, not straightforward because the biochemical profile of hop batches can vary by function of growth location and processing and storage conditions.…”

Section: Introductionmentioning

confidence: 99%

“…Several research groups have reported on the potential of molecular markers for identification and authenticity control of hop batches, as these are independent of environmental, processing, or storage conditions. Different types of molecular markers have been investigated such as random amplified polymorphic DNA (RAPD), , amplified fragment length polymorphism (AFLP), − microsatellites or simple sequence repeats (SSR), ,,,− sequence-tagged site (STS) markers, , and diversity array technology (DArT) markers . Nowadays, single nucleotide polymorphisms (SNPs) are the most commonly used molecular markers to assess genetic variation in plants.…”

Section: Introductionmentioning

confidence: 99%

“…9−12 Therefore, quality control of a given hop batch supplied to the brewer is essential, especially on varietal origin. 13,14 Distinction between varieties can be based on plant morphological characteristics and the distinctive profile of biochemical components such as hop bitter acids, essential oils, or polyphenols. 15−20 However, the varietal origin of processed hop products used in brewing practice can hardly be determined unequivocally by observation of their external appearance, and in current brewing practice, determination of varietal origin of hop batches is mostly based on biochemical markers such as the content of hop bitter acids and essential oils.…”

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Single Nucleotide Polymorphisms and Biochemical Markers As Complementary Tools To Characterize Hops (Humulus lupulus L.) in Brewing Practice

Holle

Muylle

Ruttink

et al. 2019

J. Agric. Food Chem.

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In brewing practice, the use of the appropriate hop variety is essential to produce consistent and high-quality beers. Yet, hop batches of the same variety cultivated in different geographical regions can display significant biochemical differences, resulting in specific taste- and aroma-related characteristics in beer. In this study, we illustrate the complementarity of genetic and biochemical fingerprinting methods to fully characterize hop batches. Using genotyping-by-sequencing (GBS), a set of 1 830 polymorphic single nucleotide polymorphism (SNP) markers generated 48 unique genetic fingerprints for a collection of 56 commercial hop varieties. Three groups of varieties, consisting of somaclonal variants, could not be further differentiated using this set of markers. Biochemical marker information offered added value to characterize hop samples from a given variety grown at different geographical locations. We demonstrate the power of combining genetic and biochemical fingerprints for quality control of hop batches in the brewing industry.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Single Nucleotide Polymorphisms and Biochemical Markers As Complementary Tools To Characterize Hops (Humulus lupulus L.) in Brewing Practice

Holle

Muylle

Ruttink

et al. 2019

J. Agric. Food Chem.

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“…Standardně se však využívají přímo v reakcích jednoduchých sekvenčních repetic (SSR). Proto byly a jsou SSR markery nejpoužívanější pro genotypování a studium molekulární variability u chmele (Jakše et al, 2001;2002;Čerenak et al, 2004;Hadonou et al, 2004;Murakami et al, 2006a;Bassil et al, 2008;Štajner et al, 2005;Peredo et al, 2010;Patzak et al, 2010a;Horreo et al, 2014;Karlsson Strese et al, 2014;Mongelli et al, 2015;Korbecka-Glinka et al, 2016). Většina SSR markerů se nachází v nekódujících oblastech genomu.…”

Section: ■ 3 Genetické Metodyunclassified

Methods for verifying the authenticity of hops - an effective tool against falsification

Olšovská¹,

Krofta²,

Jandovská³

et al. 2016

Kvasny Prum

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■ 1 ÚVODAutenticita potravin je termín, který jednoduše označuje, zda potraviny nakupované spotřebitelem odpovídají jejich popisu, a je aktuálním tématem v nejrůznějších potravinářských komoditách. Proto je v současné době věnováno mnoho pozornosti a jsou vynakládány nemalé fi nanční prostředky na vývoj analytických metod pro ověřo-vání autenticity potravin, nápojů a jejich surovin.Také chmel je komodita, ve které dochází k falzifi kaci původu a odrůd, což může ve velké míře ovlivnit senzorický profi l piva z něho vyrobeného. Žatecký poloraný červeňák (ŽPČ) je na trhu všeobec-ně považován za světový standard kvality v kategorii aromatických chmelů. Prvotřídní kvalitě odpovídá i vyšší cena. V uplynulých letech bylo zaznamenáno několik opakovaných případů jeho falzifi kace. Falzifi kace Žateckého poloraného červeňáku ve formě granulí byla Recenzovaný článek / Reviewed Paper Olšovská, J., Krofta, K., Jandovská, V., Patzak, J., Štěrba, K., 2016: Metody pro ověřování autenticity chmele -účinný nástroj proti falzifi kaci. Kvasny Prum. 62, č. 10, s. 294-305 K pokusům o falzifi kaci dochází téměř u všech komodit a výjimkou není ani chmel. Je proto nezbytné hledat způsoby, jak ověřovat jeho autenticitu. Přehledový článek shrnuje moderní metody pro ověřování autenticity chmele a uvádí rovněž nejnovější výsledky spolupráce Výzkumného ústavu pivovarského a sladařského, a.s. a Chmelařského institutu, s.r.o., v Žatci. K ověřování autenticity chmele se používají metody chemotaxonomické a genetické. V roce 2015 byly provedeny chemické analýzy novošlechtěných hybridů pro České pivo (obsah a složení alfa a beta kyselin, obsah a složení chmelových silic, obsah celkových polyfenolů) a byly provedeny molekulárně-genetické analýzy 150 vybraných genotypů chmele ze světového sortimentu a šlechtitelského materiálu. Pomocí výsledků byl z testovaných genotypů sestaven přehledný dendrogram. Autoři se dále zabývali profi lováním českých odrůd chmele na základě chemických profi lů proanthokyanidinů a podařilo se jim pomocí klastrové analýzy jasně odlišit odrůdy chmele včetně jejich genetické příbuznosti.Olšovská, J., Krofta, K., Jandovská, V., Patzak, J., Štěrba, K., 2016: Methods for verifying the authenticity of hops -an effective tool against falsifi cation. Kvasny Prum. 62, No. 10, pp. 294-305Falsifi cation attempts occur in almost all commodities and hops is no exception. It is therefore necessary to look for ways to verify its authenticity. This review summarizes modern methods for verifying the authenticity of hops and also provides the latest results of the collaboration of the Research Institute of Brewing and Malting, PLC. and the Hop Research Institute, Ltd., in Žatec. Chemotaxonomic and genetic methods are used to verify the authenticity of hops. Chemical analyzes of newly bred hybrids potentially suitable for Czech beer focused on the content and composition of alpha and beta acids, content and composition of hop oils, and content of total polyphenols were performed and molecular genetic analyses of 150 selected hop genotypes in...

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“…The collection is highly diverse and contains European cultivars, regional landraces, and wild native North American accessions. At the NCGR, each clonal accession is propagated vegetatively and could easily be mis-identified if solely based on morphology [3], since morphology can change based on environmental influences such as growing conditions [8,[19][20][21]. An economic DNA-based tool to confirm cultivar identity should be useful for management at the USDA and any other germplasm collection.…”

Section: Introductionmentioning

confidence: 99%

Two fingerprinting sets for Humulus lupulus based on KASP and microsatellite markers

et al. 2022

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Verification of clonal identity of hop (Humulus lupulus L.) cultivars within breeding programs and germplasm collections is vital to conserving genetic resources. Accurate and economic DNA-based tools are needed in dioecious hop to confirm identity and parentage, neither of which can be reliably determined from morphological observations. In this study, we developed two fingerprinting sets for hop: a 9-SSR fingerprinting set containing high-core repeats that can be run in a single PCR reaction and a kompetitive allele specific PCR (KASP) assay of 25 single nucleotide polymorphisms (SNPs). The SSR set contains a sex-linked primer pair, HI-AGA7, that was used to genotype 629 hop accessions from the US Department of Agriculture (USDA) National Clonal Germplasm Repository (NCGR), the USDA Forage Seed and Cereal Research (FSCR), and the University of Nebraska-Lincoln (UNL) collections. The SSR set identified unique genotypes except for 89 sets of synonymous samples. These synonyms included: cultivars with different designations, the same cultivars from different sources, heat-treated clones, and clonal variants. Population structure analysis clustered accessions into wild North American (WNA) and cultivated groups. Diversity was slightly higher in the cultivated samples due to larger sample size. Parentage and sib-ship analyses were used to identify true-to-type cultivars. The HI-AGA7 marker generated two male- and nine female-specific alleles among the cultivated and WNA samples. The SSR and KASP fingerprinting sets were compared in 190 samples consisting of cultivated and WNA accession for their ability to confirm identity and assess diversity and population structure. The SSR fingerprinting set distinguished cultivars, selections and WNA accessions while the KASP assays were unable to distinguish the WNA samples and had lower diversity estimates than the SSR set. Both fingerprinting sets are valuable tools for identity confirmation and parentage analysis in hop for different purposes. The 9-SSR assay is cost efficient when genotyping a small number of wild and cultivated hop samples (<96) while the KASP assay is easy to interpret and cost efficient for genotyping a large number of cultivated samples (multiples of 96).

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Cultivar identification in dry hop cones and pellets using microsatellite loci

Cited by 7 publications

References 27 publications

Single Nucleotide Polymorphisms and Biochemical Markers As Complementary Tools To Characterize Hops (Humulus lupulus L.) in Brewing Practice

Single Nucleotide Polymorphisms and Biochemical Markers As Complementary Tools To Characterize Hops (Humulus lupulus L.) in Brewing Practice

Methods for verifying the authenticity of hops - an effective tool against falsification

Two fingerprinting sets for Humulus lupulus based on KASP and microsatellite markers

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