Cultivar-biased regulation of HSFA7 and HSFB4a govern high-temperature tolerance in tomato

Rao, Sombir; Das, Jaishri Rubina; Balyan, Sonia; Verma, Radhika; Mathur, Saloni

doi:10.1007/s00425-021-03813-y

Cited by 11 publications

(9 citation statements)

References 71 publications

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“… HSFA1a tomato HSFA1-type HSF can promote its activity in HSE for stress protection Mesihovic A, et al 2021. [ 26 ] HsfA2 tomato Accumulation of HsfA2 by moderate HS treatment enhances the ability of seedlings to cope with subsequent severe HS Fragkostefanakis S. et al 2016 [ 27 ] HSFB4a bread wheat Mitigation of HSFB4a regulates downstream heat stress response genes to control heat tolerance in heat tolerant species Rao S.et al 2022 [ 28 ] TdHSP101C bread wheat Expression of TdHSP101 gene is associated with increased heat tolerance Bento M.et al 2017. [ 29 ] TaHSFA6e bread wheat TaHSFA6e TF can be used as a promising candidate gene for manipulating heat stress tolerance networks Kumar RR.…”

Section: Resultsmentioning

confidence: 99%

Combined transcriptomic and metabolomic analyses of high temperature stress response of quinoa seedlings

et al. 2023

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Background Quinoa (Chenopodium quinoa Willd.) originates in high altitude areas, such as the Andes, and has some inherent characteristics of cold, drought, and salinity tolerance, but is sensitive to high temperature. Results To gain insight into the response mechanism of quinoa to high temperature stress, we conducted an extensive targeted metabolomic study of two cultivars, Dianli-3101 and Dianli-3051, along with a combined transcriptome analysis. A total of 794 metabolites and 54,200 genes were detected, in which the genes related to photosynthesis were found down-regulated at high temperatures, and two metabolites, lipids and flavonoids, showed the largest changes in differential accumulation. Further analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and transcription factors revealed that quinoa inhibits photosynthesis at high temperatures, and the possible strategies being used for high temperature stress management are regulation of heat stress transcription factors (HSFs) to obtain heat tolerance, and regulation of purine metabolism to enhance stress signals for rapid response to high temperature stress. The tolerant genotype could have an enhanced response through lower purine levels. The induction of the stress response could be mediated by HSF transcription factors. The results of this study may provide theoretical references for understanding the response mechanism of quinoa to high temperature stress, and for screening potential high temperature tolerant target genes and high temperature tolerant strains. Conclusions These findings reveal the regulation of the transcription factor family HSF and the purinergic pathway in response to high temperature stress to improve quinoa varieties with high temperature tolerance.

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Section: Resultsmentioning

confidence: 99%

Combined transcriptomic and metabolomic analyses of high temperature stress response of quinoa seedlings

et al. 2023

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“…ABA inhibits the expression of miR159b, which downregulates the expression of its target genes, such as GAMYB1 , MYB29 -like, and HSP70 , to enhance high temperature tolerance in grafted cucumber plants ( Li et al, 2016 ). Heat stress induces the expression of miR4200 to degrade the mRNA of HSFB4a , a negative regulator in heat stress, thereby exhibiting higher heat stress tolerance in tomato ( Rao et al, 2022 ). Similarly, HSFA1b and HSFA7b induce miR398 to increase thermotolerance of Arabidopsis through downregulation the expression of CSD1 , CSD2, and CCS ( Guan et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

CsbZIP2-miR9748-CsNPF4.4 Module Mediates High Temperature Tolerance of Cucumber Through Jasmonic Acid Pathway

Chen

Yuan

et al. 2022

Front. Plant Sci.

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High temperature stress seriously affects the growth of cucumber seedlings, and even leads to a decline in yield and quality. miRNAs have been shown to be involved in regulating the response to stress in plants, but little is known about its effects on cucumber high temperature stress tolerance. Here, we found that high temperature stress induced the expression of miR9748 in cucumber. Overexpression of cucumber miR9748 in Arabidopsis improved high temperature tolerance. Transcriptome analysis revealed that miR9748 might mediate high temperature tolerance through plant hormone signal pathway. 5′ RNA ligase-mediated rapid amplification of cDNA ends (5′ RLM-RACE) and transient transformation technology demonstrated that CsNPF4.4 was the target gene of miR9748. CsNPF4.4 overexpression plants decreased high temperature tolerance accompanied by reducing the content of jasmonic acid (JA), but alleviated by foliar application of methyl jasmonate, indicating that CsNPF4.4 negatively regulated high temperature stress tolerance through inhibition JA signal pathway. Furthermore, high temperature stress also increased the expression level of CsbZIP2. Yeast one-hybrid and dual-luciferase assays showed that CsbZIP2 directly bound to the promoter of MIR9748 to induce its expression. Taken together, our results indicated that CsbZIP2 directly regulated miR9748 expression to cleave CsNPF4.4 to mediate high temperature tolerance through JA pathway.

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“…Sly-HSFA7, Sly-HSFA6b, Sly-HSFA4c, Sly-HSFB1 and Sly-HSFB2b act as downstream targets of Sly-HSFA1a (Rao et al, 2021). Sly-HSFA7 and Sly-HSFB4a have been shown as positive and negative regulators of heat stress in tomato, respectively (Rao et al, 2022). Moreover, Mesihovic et al (2022) have shown that HSFA7 interacts with HSFA1a to form a co-activator complex under severe heat stress conditions leading to the induction of genes required for thermotolerance.…”

Section: Introductionmentioning

confidence: 99%

“…In plants, there are multiple genes coding for HSFs; for example, Arabidopsis thaliana has 21 HSF family members (Von Koskull‐Döring et al., 2007) and tomato ( Solanum lycopersicum ) has 26 HSFs (Yang et al., 2016). The tomato heat stress transcription factor A1a (Sly‐HSFA1a) acts as the master regulator of the heat stress response by directly regulating the transcription of Sly‐HSFA2 (Rao et al., 2022). Sly‐HSFA7, Sly‐HSFA6b, Sly‐HSFA4c, Sly‐HSFB1 and Sly‐HSFB2b act as downstream targets of Sly‐HSFA1a (Rao et al., 2021).…”

Section: Introductionmentioning

confidence: 99%

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A conserved HSF:miR169:NF‐YA loop involved in tomato and Arabidopsis heat stress tolerance

et al. 2022

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Heat stress transcription factors (HSFs) and microRNAs (miRNAs) regulate different stress and developmental networks in plants. Regulatory feedback mechanisms are at the basis of these networks. Here, we report that plants improve their heat stress tolerance through HSF-mediated transcriptional regulation of MIR169 and post-transcriptional regulation of Nuclear Factor-YA (NF-YA) transcription factors. We show that HSFs recognize tomato (Solanum lycopersicum) and Arabidopsis MIR169 promoters using yeast one-hybrid/ chromatin immunoprecipitation-quantitative PCR. Silencing tomato HSFs using virus-induced gene silencing (VIGS) reduced Sly-MIR169 levels and enhanced Sly-NF-YA9/A10 target expression. Further, Sly-NF-YA9/A10 VIGS knockdown tomato plants and Arabidopsis plants overexpressing At-MIR169d or At-nf-ya2 mutants showed a link with increased heat tolerance. In contrast, Arabidopsis plants overexpressing At-NF-YA2 and those expressing a non-cleavable At-NF-YA2 form (miR169d-resistant At-NF-YA2) as well as plants in which At-miR169d regulation is inhibited (miR169d mimic plants) were more sensitive to heat stress, highlighting NF-YA as a negative regulator of heat tolerance. Furthermore, post-transcriptional cleavage of NF-YA by elevated miR169 levels resulted in alleviation of the repression of the heat stress effector HSFA7 in tomato and Arabidopsis, revealing a retroactive control of HSFs by the miR169:NF-YA node. Hence, a regulatory feedback loop involving HSFs, miR169s and NF-YAs plays a critical role in the regulation of the heat stress response in tomato and Arabidopsis plants.

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Cultivar-biased regulation of HSFA7 and HSFB4a govern high-temperature tolerance in tomato

Cited by 11 publications

References 71 publications

Combined transcriptomic and metabolomic analyses of high temperature stress response of quinoa seedlings

Combined transcriptomic and metabolomic analyses of high temperature stress response of quinoa seedlings

CsbZIP2-miR9748-CsNPF4.4 Module Mediates High Temperature Tolerance of Cucumber Through Jasmonic Acid Pathway

A conserved HSF:miR169:NF‐YA loop involved in tomato and Arabidopsis heat stress tolerance

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