Cultivable Microbiota Associated with Gold Ore from the Rozália Gold Mine, Hodruša-Hámre, Slovakia

Nosalova, Lea; Maliničová, Lenka; Kisková, Jana; Timková, Ivana; Sedláková-Kaduková, Jana; Pristaš, Peter

doi:10.1080/01490451.2021.1871685

Cited by 6 publications

(6 citation statements)

References 69 publications

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“…The present study follows our previous work of Timkova et al ( 2020 ), which demonstrated a positive correlation between increased ampicillin-chloramphenicol and nickel-copper tolerance as well as a linkage between increased tetracycline-kanamycin and zinc-lead tolerance in the bacterial community from the mine subsurface environment (Rozália Gold Mine in Hodruša-Hámre village, Slovakia). The RB2-047 strain isolated from the same environment was identified as Acinetobacter johnsonii by polyphasic approach—a combination of MALDI-TOF MS (Nosáľová et al 2021 ) as well as 16S rRNA gene and whole-genome sequence analysis (this study). It showed multiple antibiotic resistance (to ampicillin, kanamycin, chloramphenicol, tetracycline and ciprofloxacin) and high tolerance to several heavy metals (Zn, Ni, Cu and Pb).…”

Section: Discussionmentioning

confidence: 98%

“…The collected ore material was characterized by high concentrations of metals such as Zn (1,455 mg/kg), Fe (35,902 mg/kg), Mn (21,967 mg/kg), Cd (9 mg/kg) and Pb (653 mg/kg) (Timková et al 2020 ). The primary identification of the isolate was performed using Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) and it was identified as Acinetobacter johnsonii with the score value of 2.188 according to our previous study (Nosáľová et al 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…The 16S rRNA gene was amplified according to the protocol of Nosáľová et al ( 2021 ). PCR amplicons were sequenced in both directions using the Sanger sequencing method by Eurofins Genomics (Köln, Germany).…”

Section: Methodsmentioning

confidence: 99%

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Genomic insights into the adaptation of Acinetobacter johnsonii RB2-047 to the heavy metal-contaminated subsurface mine environment

Timková,

Maliničová,

Nosáľová

et al. 2023

Biometals

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The subsurface mine environments characterized by high levels of toxic metals and low nutrient availability represent an extreme threat to bacterial persistence. In recent study, the genomic analysis of the Acinetobacter johnsonii strain RB2-047 isolated from the Rozália Gold Mine in Slovakia was performed. As expected, the studied isolate showed a high level of heavy metal tolerance (minimum inhibitory concentrations were 500 mg/L for copper and nickel, 1,500 mg/L for lead, and 250 mg/L for zinc). The RB2-047 strain also showed noticeable resistance to several antibiotics (ampicillin, kanamycin, chloramphenicol, tetracycline and ciprofloxacin). The genomic composition analysis demonstrated a low number of antibiotic and metal resistance coding genes, but a high occurrence of efflux transporter genes located on the bacterial chromosome. The experimental inhibition of efflux pumps resulted in decreased tolerance to Zn and Ni (but not to Cu and Pb) and to all antibiotics tested. In addition, the H33342 dye-accumulation assay confirmed the high efflux activity in the RB2-047 isolate. These findings showed the important role of efflux pumps in the adaptation of Acinetobacter johsonii strain RB2-047 to metal polluted mine environment as well as in development of multi-antibiotic resistance.

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Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

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Genomic insights into the adaptation of Acinetobacter johnsonii RB2-047 to the heavy metal-contaminated subsurface mine environment

Timková,

Maliničová,

Nosáľová

et al. 2023

Biometals

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“…Acetylene-based collectors have garnered interest for their high reactivity, enabling strong chemical bonding with sulfides or other reactive sites on mineral surfaces, thereby enhancing mineral flotation performance, particularly in complex ore separation [16]. It was hypothesized that the acetylene group's hydrogen may interact with metals like Cu, Au, and Ag in aqueous solutions, forming organometallic compounds [17]. Burdonov et al [18] explored a novel collector, 2-methyl-3-butyn-2-ol, which shows promise in augmenting gold recovery from challenging-to-float sulfide minerals through electrostatic adsorption on pyrite surfaces.…”

Section: Introductionmentioning

confidence: 99%

Interactions of Acetylene-Derived Thioester Collectors with Gold Surfaces: A First-Principles Study

Qiu,

Qi,

Wei

et al. 2024

Minerals

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The high reactivity of the acetylene group enables the formation of strong chemical bonds with active sites on mineral surfaces, thereby improving the flotation performance of gold minerals. This study utilized density functional theory (DFT) to analyze the quantum chemical parameters of structure, Mulliken population, and the frontier orbitals of a thioester collector containing an acetylene group, PDEC (prop-2-yn-1-yl diethylcarbamodithioate). PDEC was compared with analogous thioester collectors Z-200 and Al-DECDT. The interaction mechanism of PDEC on the Au(1 1 1) surface was simulated, followed by empirical validation through adsorption experiments. The findings indicate that the S atom of PDEC in the carbon–sulfur group exhibits shorter covalent bond lengths, and has reduced carbon–sulfur double bonds and Mulliken population, resulting in enhanced electron localization. This confers greater selectivity to PDEC during its adsorption on mineral surfaces. Frontier orbital analysis shows that the electrons of the acetylene group possess a notable electron-accepting capacity, significantly influencing the frontier orbital energy of PDEC and playing a pivotal role in the bonding interaction with mineral surfaces. Both the S atom in the carbon–sulfur group and its acetylene group establish stable adsorption structures with the A(111) surface in a single coordination mode. The adsorption energy sequence is PDEC > Al-DECDT > Z-200. Partial density of states demonstrates that the S 3p orbit of the carbon–sulfur group hybridizes with the Au 5d orbit, while the C 2p orbit of the acetylene group engages in weaker back-donation bonding with the Au 5d orbit. This is corroborated by the electron density difference and post-adsorption Mulliken population analyses, revealing that the S atom of the carbon–sulfur group in PDEC donates electrons to the Au atom, forming dominant positive coordination bonds, whereas the acetylene group accepts partial electrons from the Au atom, resulting in weaker back-donation bonds. The adsorption experiments align with the DFT adsorption energy results.

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“…Thus, understanding the diversity and structure of microbial communities in rich metal areas, such as gold mines, is currently of high scientific, industrial and applied relevance. Nonetheless, microbial studies in such settings are still scarce, and while a considerable amount of information is available for prokaryotes, much less is known for fungal communities [6,22,[26][27][28][29][30][31][32][33][34][35][36][37][38]. Commonly, the study of bacteria and archaea in gold mining environments focuses on their uniqueness and phylogenetic diversity in these settings [6,26,27,30,[32][33][34][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

A Snapshot of the Microbiome of a Portuguese Abandoned Gold Mining Area

Trovão,

Soares,

Paiva

et al. 2023

Applied Sciences

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Microbial communities are known to contribute deeply to geochemical cycles, including weathering, protection from erosion and mineral precipitation. Studies aiming to understand mining areas’ microbiomes are of high relevance since they can help pinpoint the occurrence of environmental shifts, key bioremediation species, environmental metals recovery strategies, and microorganisms with relevant industrial properties. Nonetheless, so far, the study of Portuguese gold-rich areas’ microbiomes has been largely neglected. The main goal of this study was to apply high-throughput sequencing methods to study the microbiome (Bacteria and Fungi) and predict their functional/metabolic profiles in an abandoned Portuguese gold mining area (considering zones without a history of mining, the tailings and the flooded mine interior). The results obtained revealed high bacterial and fungal diversities at these sites while also pinpointing the presence of relative homogenous bacterial and heterogenous fungal communities. Areas without mining history were mainly dominated by WD2101 soil groups, Sphingomonas, Candidatus Solibacter, Helotiales, unclassified Fungi and Arxotrichum. The tailings were mainly colonized by Bryobacter, WD2101 soil groups, WPS-2 genera, Starmerella, Helotiales and Mollisia. On the other hand, the mine area displayed a dominance of Crossiella, Gemmataceae, Acidobacteriaceae (Subgroup 1), Acidiphilium, Mortierella, unclassified Fungi and Chaetothyriales. Furthermore, we verified that contrary to bacteria, the fungal structural diversity is somewhat more restricted to each site. In addition, metabolic, functional and ecological profiles revealed a strong distinction for both bacterial and fungal communities while also revealing the presence of well-adapted communities to each of the particular microenvironments considered.

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Cultivable Microbiota Associated with Gold Ore from the Rozália Gold Mine, Hodruša-Hámre, Slovakia

Cited by 6 publications

References 69 publications

Genomic insights into the adaptation of Acinetobacter johnsonii RB2-047 to the heavy metal-contaminated subsurface mine environment

Genomic insights into the adaptation of Acinetobacter johnsonii RB2-047 to the heavy metal-contaminated subsurface mine environment

Interactions of Acetylene-Derived Thioester Collectors with Gold Surfaces: A First-Principles Study

A Snapshot of the Microbiome of a Portuguese Abandoned Gold Mining Area

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