Cultivable Microbial Diversity Associated With Cellular Phones

Kurli, Rashmi; Chaudhari, Diptaraj; Pansare, Aabeejjeet N.; Khairnar, Mitesh; Shouche, Yogesh S.; Rahi, Praveen

doi:10.3389/fmicb.2018.01229

Cited by 30 publications

(25 citation statements)

References 42 publications

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“…In study of Kurli R. et al [ 32 ], matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry was used to generate protein profiles for 527 isolates obtained from cellular phones by swabs. A dendrogram was constructed based on the protein profiles of the remaining isolates, to group 112 isolates under 39 different proteotypes.…”

Section: Discussionmentioning

confidence: 99%

Degree of Bacterial Contamination of Mobile Phone and Computer Keyboard Surfaces and Efficacy of Disinfection with Chlorhexidine Digluconate and Triclosan to Its Reduction

Koščová

Hurníková

Pistl

2018

IJERPH

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The main aim of our study was to verify the effectiveness of simple disinfection using wet wipes for reduction of microbial contamination of mobile phones and computer keyboards. Bacteriological swabs were taken before and after disinfection with disinfectant wipes with active ingredients chlorhexidine digluconate and triclosan. The incidence and type of microorganisms isolated before and after disinfection was evaluated; the difference was expressed as percentage of contamination reduction. Our results confirmed the high degree of surface contamination with bacteria, some of which are opportunistic pathogens for humans. Before the process of disinfection, on both surfaces, mobile phones, and computer keyboards, the common skin commensal bacteria like coagulase-negative staphylococci were diagnosed most frequently. On the keyboards, species of the genus Bacillus and representatives of the family Enterobacteriaceae were abundant. The potentially pathogenic species were represented by Staphylococcus aureus. Cultivation of swabs performed 5 min after disinfection and subsequent calculation of the reduction of contamination have shown that simple wiping with antibacterial wet wipe led to a significant reduction of microbial contamination of surfaces, with effect ranging from 36.8 to 100%.

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Section: Discussionmentioning

confidence: 99%

Degree of Bacterial Contamination of Mobile Phone and Computer Keyboard Surfaces and Efficacy of Disinfection with Chlorhexidine Digluconate and Triclosan to Its Reduction

Koščová

Hurníková

Pistl

2018

IJERPH

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“…There is an increasing interest in studying forensically relevant microbial profiles left behind on objects and surfaces. For instance, several studies showed that there is often a high level of bacterial presence on personal objects such as mobile phones ( Koroglu et al, 2015 ; Kõljalg et al, 2017 ; Koscova et al, 2018 ; Kurli et al, 2018 ). Furthermore, human-associated items such as shoes and mobile phones have been shown to support distinct microbiomes ( Lax et al, 2015 ; Coil et al, 2019 ).…”

Section: Major Themes In the Forensic Microbiology Literaturementioning

confidence: 99%

Forensic Applications of Microbiomics: A Review

et al. 2021

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The rise of microbiomics and metagenomics has been driven by advances in genomic sequencing technology, improved microbial sampling methods, and fast-evolving approaches in bioinformatics. Humans are a host to diverse microbial communities in and on their bodies, which continuously interact with and alter the surrounding environments. Since information relating to these interactions can be extracted by analyzing human and environmental microbial profiles, they have the potential to be relevant to forensics. In this review, we analyzed over 100 papers describing forensic microbiome applications with emphasis on geolocation, personal identification, trace evidence, manner and cause of death, and inference of the postmortem interval (PMI). We found that although the field is in its infancy, utilizing microbiome and metagenome signatures has the potential to enhance the forensic toolkit. However, many of the studies suffer from limited sample sizes and model accuracies, and unrealistic environmental settings, leaving the full potential of microbiomics to forensics unexplored. It is unlikely that the information that can currently be elucidated from microbiomics can be used by law enforcement. Nonetheless, the research to overcome these challenges is ongoing, and it is foreseeable that microbiome-based evidence could contribute to forensic investigations in the future.

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“…Therefore, agreement between MALDI-TOF MS-based biotyping and 16S rRNA gene sequencing was observed to be limited. While the emerging MALDI biotyping is being popular in different fields of microbiology that has partly replaced conventional biochemical identification methods [12], variability of this methodology for the identification of microbes has been attributed to factors such as sample processing methods, matrix solutions, spectral quality, and data analysis methods [6,21]. The methodology is becoming more popular in clinical set-ups for speedy and low-cost microbial identification [28,34] and also in the analysis of bioactive compounds [46].…”

Section: Comparative Assessment Of Psychrotolerant Bacteria Using 16smentioning

confidence: 99%

16S rRNA gene sequencing and MALDI-TOF mass spectrometry based comparative assessment and bioprospection of psychrotolerant bacteria isolated from high altitudes under mountain ecosystem

et al. 2019

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The Himalayan Mountains are placed among the globally recognized biodiversity hot spots. While the Indian Himalayan Region (IHR) has been subjected to extensive studies on plant and animal biodiversity, microbial diversity is now being studied for its bioprospection. The present paper deals with the evaluation of bacterial diversity in high-altitude soil samples from IHR following polyphasic approach including comparison between the MALDI-TOF mass spectrometry and 16S rRNA gene sequencing for species-level identification. Initially, a culture collection of large number of bacterial isolates was established in the laboratory. Performing morphological and biochemical screenings, sixty-one representative isolates were selected for mass spectrometry and gene sequencing. Both the methods emerged with bacterial identification showing maximum number of Bacillus followed by Pseudomonas species. The other frequently isolated strains belonged to the genera Alcaligenes, Carnobacterium, Lysinibacillus, Microbacterium, Paenarthrobacter, Rhodococcus, Serratia and Stenotrophomonas. Although the MALDI-TOF technique appeared to be advantageous as less time-consuming in comparison with 16S rRNA-based method, the discrepancies at species level indicated the limited database of MALDI Biotyper and species complexity in the genera. The remarkable characteristics of the bacterial isolates were their tolerance to wide range of pH and temperature. Their potential to produce industrially valuable enzymes indicated their importance in bioprospection. Accessioning of these bacterial isolates in microbial culture collections is a cautious effort for their availability to conduct advanced research on these cold-adapted bacteria in future.

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Cultivable Microbial Diversity Associated With Cellular Phones

Cited by 30 publications

References 42 publications

Degree of Bacterial Contamination of Mobile Phone and Computer Keyboard Surfaces and Efficacy of Disinfection with Chlorhexidine Digluconate and Triclosan to Its Reduction

Degree of Bacterial Contamination of Mobile Phone and Computer Keyboard Surfaces and Efficacy of Disinfection with Chlorhexidine Digluconate and Triclosan to Its Reduction

Forensic Applications of Microbiomics: A Review

16S rRNA gene sequencing and MALDI-TOF mass spectrometry based comparative assessment and bioprospection of psychrotolerant bacteria isolated from high altitudes under mountain ecosystem

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