Cul3‐Klhl18 ubiquitin ligase modulates rod transducin translocation during light‐dark adaptation

Chaya, Taro; Tsutsumi, Ryotaro; Varner, Leah R.; Yamato, Masaki; Yoshida, Satoyo; Furukawa, Takahisa

doi:10.15252/embj.2018101409

Cited by 26 publications

(22 citation statements)

References 101 publications

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“…2 M Samd7 −/− , Samd11 −/− , Samd7/11 DKO, and WT control mice (n = 3) were used for the ERG study. The ERG recording method followed previously reported methods 16 . In brief, ERGs were recorded by a white LED luminescent electrode placed on the cornea (PuREC; Mayo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Functional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity

Kubo

Yamamoto

Kajimura

et al. 2021

Sci Rep

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Establishing correct neuronal cell identity is essential to build intricate neural tissue architecture and acquire precise neural function during vertebrate development. While it is known that transcription factors play important roles in retinal cell differentiation, the contribution of epigenetic factors to establishing cell identity during retinal development remains unclear. We previously reported that Samd7, a rod photoreceptor cell-specific sterile alpha motif (SAM) domain protein, functions as a Polycomb repressive complex 1 component (PRC1) that is essential for establishing rod identity. In the current study, we analyzed a functional role of Samd11, another photoreceptor-enriched SAM-domain protein, in photoreceptor differentiation and maturation. We observed that Samd11 interacts with Phc2 and Samd7, suggesting that Samd11 is a component of PRC1 in photoreceptor cells. We generated Samd11-null allele and established Samd7/11 double knock-out (DKO) mouse. The Samd7/11 DKO retina exhibits shortened photoreceptor outer segments by electron microscopy analysis. Microarray analysis revealed that Samd7/11 DKO up-regulated more retinal genes than Samd7−/− alone, partial functional redundancy of Samd7 and Samd11. Taken together, the current results suggest that Samd7 and Samd11 are PRC1 components and that Samd7 is the major regulator while Samd11 is an accessory factor used for the establishment of precise rod photoreceptor identity.

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Section: Methodsmentioning

confidence: 99%

Functional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity

Kubo

Yamamoto

Kajimura

et al. 2021

Sci Rep

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“…Electroretinograms were conducted, as described previously (Chaya et al, 2019;Kozuka et al, 2019). In brief, mice were dark-adapted overnight and then we anesthetized the mice by intraperitoneally injecting 100 mg/kg ketamine and 10 mg/kg xylazine.…”

Section: Erg Recordingmentioning

confidence: 99%

Influence of Aging on the Retina and Visual Motion Processing for Optokinetic Responses in Mice

et al. 2020

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The decline in visual function due to normal aging impacts various aspects of our daily lives. Previous reports suggest that the aging retina exhibits mislocalization of photoreceptor terminals and reduced amplitudes of scotopic and photopic electroretinogram (ERG) responses in mice. These abnormalities are thought to contribute to age-related visual impairment; however, the extent to which visual function is impaired by aging at the organismal level is unclear. In the present study, we focus on the age-related changes of the optokinetic responses (OKRs) in visual processing. Moreover, we investigated the initial and late phases of the OKRs in young adult (2–3 months old) and aging mice (21–24 months old). The initial phase was evaluated by measuring the open-loop eye velocity of OKRs using sinusoidal grating patterns of various spatial frequencies (SFs) and moving at various temporal frequencies (TFs) for 0.5 s. The aging mice exhibited initial OKRs with a spatiotemporal frequency tuning that was slightly different from those in young adult mice. The late-phase OKRs were investigated by measuring the slow-phase velocity of the optokinetic nystagmus evoked by sinusoidal gratings of various spatiotemporal frequencies moving for 30 s. We found that optimal SF and TF in the normal aging mice are both reduced compared with those in young adult mice. In addition, we measured the OKRs of 4.1G-null (4.1G–/–) mice, in which mislocalization of photoreceptor terminals is observed even at the young adult stage. We found that the late phase OKR was significantly impaired in 4.1G–/– mice, which exhibit significantly reduced SF and TF compared with control mice. These OKR abnormalities observed in 4.1G–/– mice resemble the abnormalities found in normal aging mice. This finding suggests that these mice can be useful mouse models for studying the aging of the retinal tissue and declining visual function. Taken together, the current study demonstrates that normal aging deteriorates to visual motion processing for both the initial and late phases of OKRs. Moreover, it implies that the abnormalities of the visual function in the normal aging mice are at least partly due to mislocalization of photoreceptor synapses.

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“…A recent study showed that Unc119 expression levels in photoreceptors are influenced by its phosphorylation/dephosphorylation by casein kinase 2 (CK2) and the calcium regulated calcineurin (Chaya et al, 2019 ). The phosphorylation state of Unc119 affects its interaction with cullin 3-kelch-like 18 (Cul3-Klhl18) ubiquitin E3 ligase, which ubiquitinylates unphosphorylated Unc119, leading to its degradation.…”

Section: Peripheral Membrane Protein Compartmentalization Within the mentioning

confidence: 99%

Compartmentalization of Photoreceptor Sensory Cilia

Barnes

Malhotra

Calvert

2021

Front. Cell Dev. Biol.

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Functional compartmentalization of cells is a universal strategy for segregating processes that require specific components, undergo regulation by modulating concentrations of those components, or that would be detrimental to other processes. Primary cilia are hair-like organelles that project from the apical plasma membranes of epithelial cells where they serve as exclusive compartments for sensing physical and chemical signals in the environment. As such, molecules involved in signal transduction are enriched within cilia and regulating their ciliary concentrations allows adaptation to the environmental stimuli. The highly efficient organization of primary cilia has been co-opted by major sensory neurons, olfactory cells and the photoreceptor neurons that underlie vision. The mechanisms underlying compartmentalization of cilia are an area of intense current research. Recent findings have revealed similarities and differences in molecular mechanisms of ciliary protein enrichment and its regulation among primary cilia and sensory cilia. Here we discuss the physiological demands on photoreceptors that have driven their evolution into neurons that rely on a highly specialized cilium for signaling changes in light intensity. We explore what is known and what is not known about how that specialization appears to have driven unique mechanisms for photoreceptor protein and membrane compartmentalization.

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Cul3‐Klhl18 ubiquitin ligase modulates rod transducin translocation during light‐dark adaptation

Cited by 26 publications

References 101 publications

Functional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity

Functional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity

Influence of Aging on the Retina and Visual Motion Processing for Optokinetic Responses in Mice

Compartmentalization of Photoreceptor Sensory Cilia

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