Cucumibacter marinus gen. nov., sp. nov., a marine bacterium in the family Hyphomicrobiaceae

Abstract

Cucumibacter marinus gen. nov., sp. nov., a marine bacterium in the family Hyphomicrobiaceae A Gram-negative, rod-shaped, strictly aerobic micro-organism, designated strain CL-GR60 T , was isolated from coastal seawater from the East Sea, Korea. A 16S rRNA gene sequence analysis revealed a clear affiliation with the family Hyphomicrobiaceae. Phylogenetic analyses showed that strain CL-GR60 T formed a robust cluster with the species of the genus Devosia at sequence similarity levels of 91.1-93.1 %; no other species in the family Hyphomicrobiaceae shared more than 90 % sequence similarity with strain CL-GR60 T . The strain grew optimally in the presence of 3-4 % sea salts at 30-35 6C and pH 7. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, two unidentified glycolipids, an unidentified phospholipid and an unidentified lipid. The strain contained C 18 : 1 v7c (54.7 %), 11-methyl C 18 : 1 v7c (19.3 %), C 18 : 0 (14.4 %) as the predominant fatty acids. Ubiquinone-10 was the major quinone. The DNA G+C content was 62.9 mol%. On the basis of physiological data, fatty acid composition and results of phylogenetic analysis of the 16S rRNA gene sequence, strain CL-GR60 T represents a novel genus and species within the family Hyphomicrobiaceae, for which the name Cucumibacter marinus gen. nov., sp. nov. is proposed. The type strain of Cucumibacter marinus is CL-GR60 T (5KCCM 90027 T 5DSM 18995 T ).

Zhangella mobilis gen. nov., sp. nov., a new member of the family Hyphomicrobiaceae isolated from coastal seawater

“…nov. E6 T ; 2, Cucumibacter; 3, Devosia; 4, Ahrensia; 5, Ochrobactrum. Data were taken from this study, Hwang & Cho (2008), Ryu et al (2008), Vanparys et al (2005), Rivas et al (2003), Nakagawa et al (1996), Rüger & Höfle (1992), Teyssier et al (2007), Uchino et al (1998), Tripathi et al (2006), Zurdo-Piñeiro et al (2007) and Trujillo et al (2005). All taxa were positive for catalase and oxidase activity.…”

“…Recently, members of the family Hyphomicrobiaceae have been isolated from marine environments, such as Dichotomicrobium thermohalophilum, Filomicrobium fusiforme, Hyphomicrobium aestuarii (Garrity et al, 2005) and Cucumibacter marinus (Hwang & Cho, 2008).…”

Maritalea porphyrae sp. nov., isolated from a red alga (Porphyra yezoensis), and transfer of Zhangella mobilis to Maritalea mobilis comb. nov.

“…Most of the species belonging to this family have been isolated from various non-marine habitats, such as soil and freshwater (Garrity et al, 2005). Recently, Cucumibacter marinus, Maritalea myrionectae and Zhangella mobilis have been isolated from marine environments, such as coastal seawater and a marine ciliate culture (Hwang & Cho, 2008;Hwang et al, 2009;Xu et al, 2009). M. myrionectae and Z. mobilis were separately proposed as novel species, each in a different genus, on the basis of DNA G+C content differentiating them from C. marinus (Hwang & Cho, 2008;Hwang et al, 2009;Xu et al, 2009).…”

“…The family Hyphomicrobiaceae is a member of the order Rhizobiales within the class Alphaproteobacteria and at the time of writing consists of 18 genera (Garrity et al, 2005;Lee et al, 2005;Hwang & Cho, 2008;Hwang et al, 2009;Xu et al, 2009). Most of the species belonging to this family have been isolated from various non-marine habitats, such as soil and freshwater (Garrity et al, 2005).…”

Marivita cryptomonadis gen. nov., sp. nov. and Marivita litorea sp. nov., of the family Rhodobacteraceae, isolated from marine habitats

“…The fatty acid methyl esters in whole cells of strains CL-SK44 T and CL-JM1 T grown on MA at 30 u C for 4 days were analysed by GC according to the instructions of the Microbial Identification System (MIDI) at the Korean Culture Center of Microorganisms (KCCM) in Seoul, Korea. The quinone system was determined according to Minnikin et al (1984) and was analysed by HPLC as described by Collins (1985), by using Cucumibacter marinus CL-GR60 T (Hwang & Cho, 2008) as a reference. The DNA G+C content was analysed by HPLC (HP 100; Hewlett Packard) analysis of deoxyribonucleosides as described by Mesbah et al (1989), after DNA extraction according to the method of Marmur (1961).…”