Cubogel as potential platform for glaucoma management

Abstract

The aim of this work is to survey the potential of cubogel as an ocular dosage form to boost the corneal permeability of Dorzolamide Hydrochloride DZ; an antiglaucomal drug. DZ-loaded cubosomal dispersions were prepared according to Box-Behnken design, where the effect of independent variables; Monoolein MO concentration (2.5, 5 and 7.5%w/w), Pluronic V R F127 concentration (0.25, 0.5 and 0.75%w/w) and magnetic stirrer speed of (400, 800 and 1200 rpm) was studied on PS (nm), Zp (ÀmV) and Q 2 h (%) respectively. The prepared formulae were characterized via drug content DC (%), particle size PS (nm), polydispersity index PDI, zeta potential Zp (ÀmV), in-vitro drug release (Q 2 h%) and finally TEM. The optimized formulation composed of: 6.13% w/w of MO, 0.75% w/w of F127 and prepared at 1200 rpm stirring speed was chosen based on the criteria of minimum PS (nm), maximum Zp (ÀmV) and minimum Q 2 h (%). Results revealed that the optimum formula showed PS of 153.3 ± 8.4 n, Zp of 32 ± 3 ÀmV and 37.78 ± 1.3% released after 2 h. Carbopol 934 (1% w/v) as gelling agent was used to prepare the optimum cubogel, which was further evaluated by DSC, ex-vivo permeation and stability studies at 4 C for three months. Moreover, in vivo studies of the optimized cubogel include; draize test, histological examination, confocal laser scanning microscopy (CLSM) and intraocular pressure (IOP) measurement. Results revealed that the optimized cubogel was considerably safe, stable and competent to corneal delivery as assured by draize and histological examination. CLSM showed a deeper penetration of more than 2.5-fold. A higher bioavailability (288.24 mg. h/ml) was attained from cubogel compared to the market product Trusopt V R eye drops (115.40 mg. h/ml) following IOP measurement. Therefore, DZ-loaded cubogel could be considered as promising delivery system to boost the transcorneal permeation hence corneal bioavailability of DZ as antiglaucomal drug.

An Fe‐MIL100 Based Drug Delivery System for pH and Glutathione Dual‐Responsive Drug Release

“…[20,21] Conventionally, the release of DOX which loaded in MIL100 could be excited by low pH condition and abundant H 2 O 2 in tumour. [22] In this study we found that lower pH and GSH can synergistically promote drug release from the DOX@PEG-MIL100 nanoparticles, thus to realize the pH and GSH dualsensitive drug deliver system.…”

Cubosomal Betamethasone-Salicylic Acid Nano Drug Delivery System for Enhanced Management of Scalp Psoriasis

“…All runs except for runs 4, 7, 8, 9 are within the accepted USP drug ranges which varies from 85–115%. 51 , 52 …”

Brain targeting efficiency of intranasal clozapine-loaded mixed micelles following radio labeling with Technetium-99m

“…Specimens were cleared by xylene and inserted in paraffin at 56 °C in hot air oven for 24 h. Sledge microtome is used to prepare paraffin bees wax tissue units at 4 µm thickness. The achieved tissue sections were gathered on glass slides, deparaffinized, stained by hematoxylin and eosin stain for usual assessment via the light electric microscope (Leica, Cambridge, UK) (Sayed et al, 2021 ).…”