Cu,Zn‐superoxide dismutase of <i>Saccharomyces cerevisiae</i> is required for resistance to hyperosmosis

Garay‐Arroyo, Adriana; Lledı́as, Fernando; Hansberg, Wilhelm; Covarrubias, Alejandra A.

doi:10.1016/s0014-5793(03)00199-6

Cited by 21 publications

(19 citation statements)

References 24 publications

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“…The SOD1Δ strain exhibited high sensitivity to paraquat and methionine auxotrophy (data not shown), that was observed with previously isolated Sod1-lacking mutants (Bilinski et al, 1985;Chang and Kosman, 1990). Osmotic stress also hampers the growth of yeast cells, and the SOD1 mutants are oversensitive to hypertonic medium (e.g., one containing 0.8 M NaCl) (Garay-Arroyo et al, 2003;Koziol et al, 2005). We compared cell growth under aerobic conditions of the SOD1Δ strain with that of wild-type yeast in YPD liquid media containing various concentrations of sorbitol, and also observed growth inhibition of the SOD1Δ strain at high concentrations of sorbitol ( Fig.…”

Section: Resultssupporting

confidence: 78%

“…3, the carbonyl content of some proteins between 40 and 70 kDa was increased by exposure to 1.7 M sorbitol, and SOD1Δ cells showed more enhanced protein carbonylation than wild-type cells. This result supports the idea that under hyperosmosis the Sod1 defect leads to a high protein oxidation level and that the mutant shows a growth arrest phenotype (Garay-Arroyo et al, 2003).…”

Section: Sod1 Sod2supporting

confidence: 88%

“…Upon osmotic shock, a number of genes encoding enzymes implicated in oxidative damage repair are also induced under the control of the HOG pathway and the general stress response (Garay-Arroyo et al, 2003;Hohmann et al, 2007). Therefore, the result suggests that the growth-arrested yeast cells could adapt to hyperosmotic stress, possibly by activation of a cellular response alternative to scavenging of endogenous ROS by Sod1.…”

Section: Sod1 Sod2mentioning

confidence: 99%

“…Hyperosmotic stress also hampers the growth of yeast cells (GarayArroyo et al, 2003). This effect is mediated by oxidative stress, since the SOD1Δ strain is oversensitive to hypertonic medium compared to wild-type yeast, and ROS accumulate in yeast cells exposed to hypertonic media (Garay-Arroyo et al, 2003;Koziol et al, 2005). These obvious phenotypes of SOD1Δ could be reversed by the addition of L-ascorbic acid (AsA), a potent antioxidant (Krzepilko et al, 2004;Zyracka et al, 2005a;Koziol et al, 2005, Saffi et al, 2006.…”

Section: Introductionmentioning

confidence: 99%

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A Simple Growth Test of a Saccharomyces cerevisiae Cu, Zn-Superoxide Dismutase-Deficient Mutant in Hypertonic Medium for Biological Evaluation of Antioxidants

Tamura¹,

Wada²,

Hase³

et al. 2010

FSTR

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Osmotic stress hampers the growth of Saccharomyces cerevisiae, and this effect is mediated by oxidative stress. A simple test for the biological evaluation of antioxidants was developed on the basis of recovery of growth delay of Cu, Zn-superoxide dismutase-lacking yeast (SOD1Δ) under hyperosmosis. The SOD1Δ strain suffered from growth inhibition in a medium containing 1.7 M sorbitol. Protein oxidation in SOD1Δ cells was greatly increased by exposure to a hypertonic medium, indicating the accumulation of reactive oxygen species followed by oxidation of cellular materials. The hyperosmosis-induced growth arrest of SOD1Δ was abolished by the addition of L-ascorbic acid to the medium; the antioxidant effect depended on the concentration, ranging from 1 to 10 mM. Cysteine, N-acetyl-cysteine, and glutathione were also able to restore the growth of SOD1Δ cells. High concentrations of these thiol compounds proved less effective, probably due to adverse effects of an excess of these antioxidants. No growth restoration was seen for typical polyphenol antioxidants, including curcumin, quercetin and catechin.

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Section: Resultssupporting

confidence: 78%

Section: Sod1 Sod2supporting

confidence: 88%

Section: Sod1 Sod2mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Simple Growth Test of a Saccharomyces cerevisiae Cu, Zn-Superoxide Dismutase-Deficient Mutant in Hypertonic Medium for Biological Evaluation of Antioxidants

Tamura¹,

Wada²,

Hase³

et al. 2010

FSTR

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“…During a hyperoxidant state, the amount and ratio of NAD(P)H/NAD(P) and glutathione/glutathione disulfide changes dramatically (40,42) and this will change many met- (34). Under these conditions, SOD is also regulated although through the protein kinase A-cyclic AMP (cAMP) and Skn7 pathways (11). In Schizosaccharomyces pombe (7) and Aspergillus nidulans (22), osmotic and oxidative stress activates the HOG1 homologues Spc1 and SakA, respectively.…”

mentioning

confidence: 99%

Asexual Development Is Increased in Neurospora crassa cat - 3 -Null Mutant Strains

2003

Self Cite

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We use asexual development of Neurospora crassa as a model system with which to determine the causes of cell differentiation. Air exposure of a mycelial mat induces hyphal adhesion, and adherent hyphae grow aerial hyphae that, in turn, form conidia. Previous work indicated the development of a hyperoxidant state at the start of these morphogenetic transitions and a large increase in catalase activity during conidiation. Catalase 3 (CAT-3) increases at the end of exponential growth and is induced by different stress conditions. Here we analyzed the effects of cat-3-null strains on growth and asexual development. The lack of CAT-3 was not compensated by other catalases, even under oxidative stress conditions, and cat-3 RIP colonies were sensitive to H 2 O 2 , indicating that wild-type (Wt) resistance to external H 2 O 2 was due to CAT-3. cat-3 RIP colonies grown in the dark produced high levels of carotenes as a consequence of oxidative stress. Light exacerbated oxidative stress and further increased carotene synthesis. In the cat-3 RIP mutant strain, increased aeration in liquid cultures led to increased hyphal adhesion and protein oxidation. Compared to the Wt, the cat-3 RIP mutant strain produced six times more aerial hyphae and conidia in air-exposed mycelial mats, as a result of longer and more densely packed aerial hyphae. Protein oxidation in colonies was threefold higher and showed more aerial hyphae and conidia in mutant strains than did the Wt. Results indicate that oxidative stress due to lack of CAT-3 induces carotene synthesis, hyphal adhesion, and more aerial hyphae and conidia.

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Current awareness on yeast

2003

Yeast

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 19th. Feb. 2003)

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Cu,Zn‐superoxide dismutase of Saccharomyces cerevisiae is required for resistance to hyperosmosis

Cited by 21 publications

References 24 publications

A Simple Growth Test of a Saccharomyces cerevisiae Cu, Zn-Superoxide Dismutase-Deficient Mutant in Hypertonic Medium for Biological Evaluation of Antioxidants

A Simple Growth Test of a Saccharomyces cerevisiae Cu, Zn-Superoxide Dismutase-Deficient Mutant in Hypertonic Medium for Biological Evaluation of Antioxidants

Asexual Development Is Increased in Neurospora crassa cat - 3 -Null Mutant Strains

Current awareness on yeast

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