CTX-M-55-Type Extended-Spectrum β-lactamase- Producing Shigella sonnei Isolated from a Korean Patient Who Had Travelled to China

Lee, Wonmok; Chung, Hae Sun; Lee, Hyukmin; Yum, Jong Hwa; Yong, Dongeun; Jeong, Seok Hoon; Lee, Kyungwon; Chong, Yunsop

doi:10.3343/alm.2013.33.2.141

Cited by 27 publications

(13 citation statements)

References 28 publications

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“…CTX-M-type ESBLs, of different genetic groups, have been reported by other studies from Spain, France, Japan and Korea, showing that the pattern of CTX-M-type ESBL genes could vary geographically ( 27 - 30 ). The results obtained by the PCR method showed that eight CTX-M-positive Klebsiella isolates harbored both blaCTX-M-1 and blaCTX-M-2 genes, and blaCTX-M-1, blaCTX-M-2 and blaCTX-M-9 genes coexisted in three CTX-M positive Klebsiella isolates.…”

Section: Discussionmentioning

confidence: 75%

Characterization of CTX-M-Type Extend-Spectrum β-Lactamase Producing Klebsiella spp. in Kashan, Iran

Afzali

Firoozeh

Amiri

et al. 2015

Jundishapur J Microbiol

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Context:The CTX-M family consists of more than 50 β-lactamases, which are grouped on the basis of sequences into five subtypes including CTX-M-1, CTX-M-2, CTX-M-8, CTX-M-9 and CTX-M-25.Objectives:The current study aimed to detect subtypes of CTX-M extended-spectrum β-lactamases (ESBLs) among ESBL positive Klebsiella isolates from patients in Kashan, Iran.Materials and Methods:A total of 100 clinical isolates of Klebsiella were collected and the isolates, which showed resistance or reduced susceptibility to cefotaxime, ceftazidime and/or aztreonam by the disk diffusion method were selected. These isolates were identified as ESBL-producing isolates by double disk synergy tests using clavulanic acid, cefotaxime, ceftazidime and aztreonam. The blaCTX-M type determinants were identified by the Polymerase Chain Reaction (PCR) method followed by DNA sequencing.Results:Of the 100 Klebsiella isolates, 41 (41%) demonstrated resistance or reduced susceptibility to ceftazidime and/or aztreonam and 35% (n = 35) were ESBL-producers. Twenty-eight (8o%) of the ESBL-producing isolates carried the blaCTX-M type genes. Based on PCR assays and sequencing of blaCTX-M genes, CTX-M-1, CTX-M-2 and CTX-M-9 were identified in 21 (60%), 15 (42%) and nine (34%) of these isolates, respectively (GenBank accession numbers KJ803828-KJ803829).Conclusions:Our study showed that the frequency of blaCTX-M genes among Klebsiella isolates in our region is at an alarming rate. Also, we found a high prevalence of blaCTX-M-1 β-lactamase in Klebsiella isolates in Kashan.

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Section: Discussionmentioning

confidence: 75%

Characterization of CTX-M-Type Extend-Spectrum β-Lactamase Producing Klebsiella spp. in Kashan, Iran

Afzali

Firoozeh

Amiri

et al. 2015

Jundishapur J Microbiol

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“…Over the past decade, the isolate rate of CTX-M-55 in Escherichia coli from animals has been increasingly raised. However, CTX-M-55 was not identified in clinical practices in China until 2010, when it was detected from a person who traveled to China [ 6 ]. Since then, plenty of surveys have confirmed the emergence of bla CTX - M - 55 among clinical pathogenic in China [ 7 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic contexts related to the diffusion of plasmid-mediated CTX-M-55 extended-spectrum beta-lactamase isolated from Enterobacteriaceae in China

Gou

Guo

et al. 2018

Ann Clin Microbiol Antimicrob

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BackgroundCTX-M-55 extended-spectrum beta-lactamases are being rapidly disseminated and transmitted in clinical practices around the world. The genetic contexts of the transferable plasmid-mediated blaCTX-M-55 gene in Enterobacteriaceae were detected and characterized in this study.MethodsIsolates were obtained from the First Affiliated Hospital of Zhengzhou University between September 2015 and March 2016. Based on polymerase chain reaction and BLAST analysis, resistance genes and genetic context of the blaCTX-M-55 gene were investigated. Conjugation experiments and multilocus sequence typing were performed to demonstrate plasmid-mediated blaCTX-M-55 transmission.ResultsThirteen blaCTX-M-55-positive isolates of Enterobacteriaceae were obtained. Seven isolates were Escherichia coli, 3 were Klebsiella pneumoniae, 1 was Citrobacter freundii, 1 was Morganella morganii and 1 was Serratia marcescens. The blaCTX-M-55 gene has not previously been identified from C. freundii and M. morganii. Four different blaCTX-M-55 genetic contexts were identified, and all of them harbored ISEcp1 in the region upstream of blaCTX-M-55 (in two cases, ISEcp1 was truncated by IS26, and in one case, it was truncated by IS1294), whereas ORF477 was detected downstream of the blaCTX-M-55 gene from 12 of 13 strains. The novel genetic context of ISEcp1∆-blaCTX-M-55-∆IS903 was firstly detected the IS903 element which was identified downstream of blaCTX-M-55. A conjugation assay revealed that all blaCTX-M-55 plasmids were quickly and easily transferable to recipient E. coli, which then presented resistance to multiple antibiotics.ConclusionsNumerous blaCTX-M-55-positive strains were isolated in a short period of 7 months. The findings indicate that blaCTX-M-55 was rapidly disseminated. The genetic context and conjugative transfer found in this study demonstrate that there is active transmission of blaCTX-M-55 among strains of Enterobacteriaceae in China, which could give rise to an urgent global public health threat.

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“…CTX-M-55 . Lee et al also reported the isolation of CTX-M-55-type ESBL-producing Shigella sonnei from a patient who had traveled to China (26). Most of these cases, including the current case, occurred in China, suggesting that CTX-M-55-producing Enterobacteriaceae are widely disseminated throughout the country.…”

Section: Discussionmentioning

confidence: 97%

Liver Abscess Caused by CTX-M-55-type Extended-spectrum β-lactamase (ESBL)-producing <i>Salmonella enteritidis</i>

et al. 2014

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Liver abscesses secondary to Salmonella species are rarely described in the general population. We herein describe a case of a liver abscess caused by CTX-M-55-type extended-spectrum β-lactamase (ESBL)-producing Salmonella enteritidis, which has not been reported in the literature. A 54-year-old male was admitted due to a high fever and was clinically diagnosed with a liver abscess. Culture of the fluid from the liver abscess revealed CTX-M-55-type ESBL-producing S. enteritidis. Although the patient underwent percutaneous transhepatic abscess drainage and antibiotic therapy, he died one month later. It should be noted that liver abscesses are potentially fatal depending on the causative pathogen.

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CTX-M-55-Type Extended-Spectrum β-lactamase- Producing Shigella sonnei Isolated from a Korean Patient Who Had Travelled to China

Cited by 27 publications

References 28 publications

Characterization of CTX-M-Type Extend-Spectrum β-Lactamase Producing Klebsiella spp. in Kashan, Iran

Characterization of CTX-M-Type Extend-Spectrum β-Lactamase Producing Klebsiella spp. in Kashan, Iran

Genetic contexts related to the diffusion of plasmid-mediated CTX-M-55 extended-spectrum beta-lactamase isolated from Enterobacteriaceae in China

Liver Abscess Caused by CTX-M-55-type Extended-spectrum β-lactamase (ESBL)-producing <i>Salmonella enteritidis</i>

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