CTX-M-15 Type β-lactamases From Clinical Isolates of Escherichia coli by Polymerase Chain Reaction and DNA Sequencing

Moghaddam, Mahboobeh Nakhaei; Zolfaghari, Mohammad Reza; Tavakoli‐Hoseini, Niloofar

doi:10.5812/zjrms.5814

Cited by 4 publications

(3 citation statements)

References 26 publications

(27 reference statements)

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“…Globally, bla CTX-M-15 had long been cited as the most prevalent variant of bla CTX-M in E. coli [27][28][29]. The high prevalence rate of bla CTX-M-15 (89.7%) observed among bla CTX-M positive strains in our study corroborates these earlier studies.…”

Section: Target Genes Sequences Genes Sizes (Bp) Annealingtemp (°C) R...supporting

confidence: 91%

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Molecular Characterization of Extended-Spectrum Beta-Lactamase-Producing Extra-Intestinal Pathogenic Escherichia coli Isolated in a University Teaching Hospital Dakar-Senegal

KM,

et al. 2022

austinjmicrobiol

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Extra-Intestinal Pathogenic Escherichia coli (ExPEC) is a predominant Gram-negative bacterial pathogen and is responsible of several diseases including Urinary Tract Infections (UTI), nosocomial pneumonia, and neonatal meningitis. ExPEC isolates are often multidrug resistant and clones producing Extended-Spectrum Beta-Lactamases (ESBL) are increasingly reported all over the world. Seventy-eight clinical ExPEC strains (49 Community-Acquired (CA) and 29 Hospital-Acquired (HA)) were selected for this study. The majority was from UTIs (n=51), while the rest (n=27) was from pus, sputum, bronchial fluid and vaginal samples (non-uropathogenic ExPEC). Antibiotic susceptibility testing was performed using the Kirby-Bauer disc diffusion method. Standard polymerase chain reaction was used to screen major ESBL genes (blaCTX-M, blaOXA-1, blaTEM, blaSHV) and blaCTX-M variants (blaCTX-M-1, blaCTX-M-9, blaCTX-M-15, blaCTX-M-25). All the tested isolates were resistant to ampicillin, ticarcillin, amoxicillin/ clavulanic acid combination, cefalotin, cefotaxime, ceftazidime, cefepime and aztreonam, but showed a high susceptibityto fosfomycin (98.7%, n = 77), ertapenem (96.2%, n = 75), and imipenem (100%). Moreover, isolates harbored at least one ESBL gene, including blaCTX-M (98.7%), blaOXA-1 (78.2%), blaTEM (44.9%) and blaSHV (3.8%). The CTX-M variants were also found with the predominance of blaCTX-M-1 (89.7%) and blaCTX-M-15 (89.7%) followed by blaCTX-M-9 (11.5%). Despite the resistance to most of the tested antibiotics, ExPEC isolates showed fortunately good susceptibility to fosfomycin and carbapenems. blaCTX-M1, blaCTX-M15 and blaOXA-1 seem to be E.coli major ESBL genes circulating in Senegal. The high level of resistance to antimicrobials observed stresses the need of establishing an epidemiological surveillance of antimicrobial resistance in both community and hospital settings.

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Section: Target Genes Sequences Genes Sizes (Bp) Annealingtemp (°C) R...supporting

confidence: 91%

“…These data suggest that bla CTX-M-15 is not the only major variant of bla CTX-M circulating in Senegal. The low prevalence rates of bla CTX-M-9 (11.5%) and bla CTX-M-25 (0%) follow trends observed in other parts of the world [27][28][29][30].…”

Section: Target Genes Sequences Genes Sizes (Bp) Annealingtemp (°C) R...supporting

confidence: 69%

Molecular Characterization of Extended-Spectrum Beta-Lactamase-Producing Extra-Intestinal Pathogenic Escherichia coli Isolated in a University Teaching Hospital Dakar-Senegal

KM,

et al. 2022

austinjmicrobiol

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“…The antibiotics used were amikacin (Ak), ceftazidime (CAZ), ceftriaxone (CRO), piperacillin/tazobactam (TZP), amoxicillin-clavulanic acid (AMC), imipenem (IPM), meropenem (MEM), cephradine (CE), ciprofloxacin (CIP), cefaclor (CEC), cefpirome (CPO), cefoperazone + sulbactam (SCF), gentamicin (GTX), doxycycline (DO), ampicillin (AMP), and co-trimoxazole (SXT). The inhibition zone of bacterial growth around the antibiotic disc was measured in mm and results were interpreted as stated by Clinical Laboratory Standard Institute (CLSI) guidelines [25]. …”

Section: Antibiotic Susceptibility Testmentioning

confidence: 99%

Antimicrobial susceptibility patterns and CTX-M β-lactamase producing clinical isolates from burn patients in Islamabad, Pakistan

Hussain¹,

Munir²,

Fatima³

et al. 2017

APJTD

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Phenotypic and molecular characterization of enteropathogenic Escherichia coli and Salmonella spp. causing childhood diarrhoea in Awka, South-Eastern Nigeria

Nwike,

Ugwu,

Ejikeugwu

et al. 2023

Bull Natl Res Cent

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Background Diarrhoea is a major cause of childhood disease in the developing countries. This experimental study investigated the prevalence of ESBL and MBL genes in enteropathogenic strains of Escherichia coli and Salmonella spp. isolated from diarrheagenic children in Awka, Nigeria. Methods Two hundred stool samples were collected from diarrhea patients in three paediatric hospitals within Awka metropolis, Nigeria. All E. coli and Salmonella spp. isolated through standard bacteriological methods were subjected to antibiotic-susceptibility testing. Double disc synergy and imipenem-EDTA combined disc tests were used to phenotypically confirm the presence of ESBL and MBL respectively. PCR amplification of β-lactamase genes was done. Results The prevalence of E. coli and Salmonella species in this study were 54% and 24.5% respectively. The organisms were highly resistant to metronidazole, cefuroxime and ceftazidime, and also showed a high sensitivity to nitrofurantoin and gentamicin. ESBL production was recorded in E. coli (49%) and Salmonella spp. (51.1%) while 27 isolates of E. coli (25%) and 7 isolates of Salmonella spp. were confirmed MBL positive by the combined disk diffusion technique. Eleven E. coli and 4 Salmonella spp. co-harbored both ESBL and MBL production. The most prevalent MBL gene in this study is the blaVIM gene (18.8%) which mediate MBL production in Gram negative bacteria; and this was followed by blaSHV (12.5%), blaTEM and blaCTX-M (6.3% each) for E. coli isolates. Salmonella spp. was recorded to have blaVIM (28.8%), blaSHV (28.8%), blaTEM (14.3%) and blaCTX-M (14.3%) genes. Conclusions This study reveals the prevalence of enteropathogenic E. coli and Salmonella strains bacteriologically recovered from diarrheic children in Awka, Nigeria, and which were found to be multiple resistant to clinically-relevant antibiotics because they co-express ESBL and MBL genes which mediate multidrug resistance in Gram negative bacteria.

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CTX-M-15 Type β-lactamases From Clinical Isolates of Escherichia coli by Polymerase Chain Reaction and DNA Sequencing

Abstract: Background: E. coli is a common cause of urinary tract infection and bacteremia in humans. That is frequently resistant to β-lactam

Cited by 4 publications

References 26 publications

Molecular Characterization of Extended-Spectrum Beta-Lactamase-Producing Extra-Intestinal Pathogenic Escherichia coli Isolated in a University Teaching Hospital Dakar-Senegal

Molecular Characterization of Extended-Spectrum Beta-Lactamase-Producing Extra-Intestinal Pathogenic Escherichia coli Isolated in a University Teaching Hospital Dakar-Senegal

Antimicrobial susceptibility patterns and CTX-M β-lactamase producing clinical isolates from burn patients in Islamabad, Pakistan

Phenotypic and molecular characterization of enteropathogenic Escherichia coli and Salmonella spp. causing childhood diarrhoea in Awka, South-Eastern Nigeria

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