CTT1 overexpression increases the replicative lifespan of MMS-sensitive Saccharomyces cerevisiae deficient in KSP1

Zhao, Wei; Zheng, Huazhen; Zhou, Tao; Hong, Xiaoshan; Cui, Hongjing; Jiang, Zhiwen; Chen, Hui-ji; Zhou, Zhongjun; Liu, Xinguang

doi:10.1016/j.mad.2017.03.008

Cited by 11 publications

(6 citation statements)

References 41 publications

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“…e PCK1 overexpression plasmid (pRS306-PCK1-OE) was constructed by inserting a 1900 bp BamHI-EcoRI fragment and a 1417 bp EcoRI-ClaI fragment amplified from yeast genomic DNA into the BamHI and ClaI sites of pRS306. In addition to the ORF of PCK1, ∼533 nucleotides of upstream sequence and ∼300 nucleotides of downstream sequence were amplified [19,20]. us, expression of PCK1 would be driven by its natural promoter.…”

Section: Pck1 Deletion and Overexpression Strains Constructionmentioning

confidence: 99%

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PCK1Deficiency Shortens the Replicative Lifespan ofSaccharomyces cerevisiaethrough Upregulation ofPFK1

Yuan

Lin

Cui

et al. 2020

BioMed Research International

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The cytosolic isozyme of phosphoenolpyruvate carboxykinase (PCK1) was the first rate-limiting enzyme in the gluconeogenesis pathway, which exerted a critical role in maintaining the blood glucose levels. PCK1 has been established to be involved in various physiological and pathological processes, including glucose metabolism, lipid metabolism, diabetes, and tumorigenesis. Nonetheless, the association of PCK1 with aging process and the detailed underlying mechanisms of PCK1 on aging are still far to be elucidated. Hence, we herein constructed the PCK1-deficient (pck1Δ) and PCK1 overexpression (PCK1 OE) Saccharomyces cerevisiae. The results unveiled that PCK1 deficiency significantly shortened the replicative lifespan (RLS) in the S. cerevisiae, while overexpression of PCK1 prolonged the RLS. Additionally, we noted that the ROS level was significantly enhanced in PCK1-deficient strain and decreased in PCK1 OE strain. Then, a high throughput analysis by deep sequencing was performed in the pck1Δ and wild-type strains, in an attempt to shed light on the effect of PCK1 on the lifespan of aging process. The data showed that the most downregulated mRNAs were enriched in the regulatory pathways of glucose metabolism. Fascinatingly, among the differentially expressed mRNAs, PFK1 was one of the most upregulated genes, which was involved in the glycolysis process and ROS generation. Thus, we further constructed the pfk1Δpck1Δ strain by deletion of PFK1 in the PCK1-deficient strain. The results unraveled that pfk1Δpck1Δ strain significantly suppressed the ROS level and restored the RLS of pck1Δ strain. Taken together, our data suggested that PCK1 deficiency enhanced the ROS level and shortened the RLS of S. cerevisiae via PFK1.

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Section: Pck1 Deletion and Overexpression Strains Constructionmentioning

confidence: 99%

“…e growth was assessed in the cell culture plates using a Bioscreen CMB instrument (Finland) [19,22,23]. Yeast cultures were prepared from a single colony and diluted in 10-fold series.…”

Section: Growth Determinationmentioning

confidence: 99%

PCK1Deficiency Shortens the Replicative Lifespan ofSaccharomyces cerevisiaethrough Upregulation ofPFK1

Yuan

Lin

Cui

et al. 2020

BioMed Research International

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“…Rapamycin slows down aging, suppresses cellular senescence, and postpone age-related diseases (Blagosklonny 2012). Further to validate the anti-aging activity, we employed specific spot assay with eukaryotic model organism Saccharomyces cerevisiae mutant strain BY4742 (MTCC-3157) as reported earlier (Zhao et al 2017). Using the method of Wei et al (2017), we observed that in the presence of ABRF2 crude extract, the formation of growth zone by yeast was higher in size as compared with control suggesting it's potential to enhance the lifespan and delay aging of yeast cell.…”

Section: Discussionmentioning

confidence: 93%

“…Traditional spot assay was employed for evaluation of the anti-aging capacity of identified compounds in eukaryotic haploid organism (Yeast) Saccharomyces cerevisiae specific mutant strain BY4742 (MTCC-3157) as described earlier (Zhao et al 2017). BY4742 strain of S. cerevisiae was inoculated in 5 mL YPD (Yeast Peptone Dextrose broth (Hi-Media, Mumbai, MH, India) and incubated overnight at 28 ± 2 °C till exponential phase.…”

Section: Anti-aging Analysismentioning

confidence: 99%

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In vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2

Sahu

Kaushik

Das

et al. 2020

Bioresour. Bioprocess.

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The present study evaluated the potential biological activities of rhizospheric fungi isolated from the Achanakmar Biosphere Reserve, India. Fungus, Talaromyces purpureogenus isolate-ABRF2 from the soil of the Achanakmar biosphere was characterized by using morphological, biochemical and molecular techniques. Fungus was screened for the production of secondary metabolites using a specific medium. The metabolites were extracted using a suitable solvent and each fraction was subsequently evaluated for their antioxidant, antimicrobial, antiproliferative and anti-aging properties. The ethanolic extract depicted the highest antioxidant activity with 83%, 79%, 80% and 74% as assessed by ferric reducing power, 2,2-diphenyl 1-picrylhydrazyl, 2,2′-azino-bis3-ethylbenzthiazoline-6-sulfonic and phosphomolybdenum assays, respectively. Similarly, ethanolic extracts depicted marked antimicrobial activity as compared with standard antibiotics and antifungal agents as well as demonstrated significant antiproliferative property against a panel of mammalian cancer cell lines. Furthermore, different fractions of the purified ethanolic extract obtained using adsorption column chromatography were evaluated for antiproliferative property and identification of an active metabolite in the purified fraction using gas chromatography-mass spectroscopy and nuclear magnetic resonance techniques yielded 3-methyl-4-oxo-pentanoic acid. Thus, the present study suggests that the active metabolite 3-methyl-4-oxo-pentanoic acid extracted from Talaromyces purpureogenus isolate-ABRF2 has a potential antiproliferative, anti-aging, and antimicrobial therapeutic properties that will be further evaluated using in vivo studies in future.

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Endosomal trafficking and DNA damage checkpoint kinases dictate survival to replication stress by regulating amino acid uptake and protein synthesis

et al. 2021

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CTT1 overexpression increases the replicative lifespan of MMS-sensitive Saccharomyces cerevisiae deficient in KSP1

Cited by 11 publications

References 41 publications

PCK1Deficiency Shortens the Replicative Lifespan ofSaccharomyces cerevisiaethrough Upregulation ofPFK1

PCK1Deficiency Shortens the Replicative Lifespan ofSaccharomyces cerevisiaethrough Upregulation ofPFK1

In vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2

Endosomal trafficking and DNA damage checkpoint kinases dictate survival to replication stress by regulating amino acid uptake and protein synthesis

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