CTR9‐mediated JAK2/STAT3 pathway promotes the proliferation, migration, and invasion of human glioma cells

Abstract

Background CTR9 (Cln three requiring 9) has been reported to be implicated in protein modification and oncogenesis of several human cancers. However, the protein expression and mechanism of CTR9 in glioma progression remain unclear. Methods We analyzed mRNA expression of CTR9 and CTR9‐related survival curves in the public database. Then, we detected CTR9 expression in glioma tissues and constructed U251 and U87 cells with stable silencing or overexpression of CTR9. Cell function tests and Western blot were conducted to explore the effects of CTR9 on glioma proliferation, invasion and migration, and the specific mechanism. All the date was presented as means ± SEM. Two‐sample t test and one‐way analysis of variance (ANOVA) were used to identify whether there was a significant difference between each group of data. Results We found that CTR9 was overexpressed in glioma and inversely associated with glioma patient survival. The results manifested that knockdown of CTR9 suppressed the proliferation, migration, and invasion of glioma cells, while overexpression facilitated them. The underlying molecular mechanism may involve the regulation of JAK2/STAT3 pathway by CTR9. Conclusion Our present study indicates that CTR9 is highly expressed in glioma and related to glioma grading and prognosis. CTR9 regulates malignant behaviors of glioma cells by activating JAK2/STAT3 pathway. Therefore, CTR9 may be a promising biomarker for the targeted therapy and prognosis evaluation of glioma.

FANCD2 inhibits ferroptosis by regulating the JAK2/STAT3 pathway in osteosarcoma

“…Panaxadiol inhibits proliferation, and induced apoptosis of pancreatic cancer cells, and suppresses the growth of xenograft models by suppressing the JAK2/STAT3 pathway [ 30 ]. In addition, the activation of JAK2/STAT3 pathway regulates malignant behaviors of glioma cells, including the proliferation and invasion of glioma cells [ 31 ]. A recent study showed that miR-19a can inhibit the JAK2/STAT3 pathway, thereby inhibiting cell proliferation and promoting apoptosis of SaOS-2 cells [ 32 ].…”

Pan-cancer analysis of homeodomain-containing gene C10 and its carcinogenesis in lung adenocarcinoma

“…These results indicated that these pathways may be inactivated in STAD, which was consistent with previous study [ 21 ]. On the contrary, HOXC10 was positively associated with IL6/JAK/STAT3 and IL2/STAT5 signaling in glioma, and previous studies also indicated that these two signaling pathways were activated [ 22 , 23 ]. We also evaluated the correlations of HOXC10 with immune infiltrations, and found that HOXC10 was positively associated with immune score in most cancers (LUAD, LUSC, TGCT, CESC, ESCA, PAAD, SARC, STAD, BRCA) except COAD.…”