CtCdc55p and CtHal3p: Two putative regulatory proteins from Candida tropicalis with long acidic domains

Rodriguez, Pedro L.; Ali, Rashid; Serrano, Ramón

doi:10.1002/(sici)1097-0061(199610)12:13<1321::aid-yea27>3.0.co;2-6

Cited by 14 publications

(6 citation statements)

References 30 publications

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“…1995 ; Ferrando et al . 1995 ), yeast open reading frame YOR053w ( ), Candida tropicalis CtHAL3 ( ) ( Rodríguez et al . 1996 ), and yeast open reading frame YKL088w ( ), using the progressive alignment method of Feng et al .…”

Section: Resultsmentioning

confidence: 99%

Arabidopsis thaliana AtHAL3: a flavoprotein related to salt and osmotic tolerance and plant growth

et al. 1999

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SummaryWe have isolated two Arabidopsis thaliana genes, AtHAL3a and AtHAL3b, showing homology with HAL3, a yeast protein which regulates the cell cycle and tolerance to salt stress through inhibition of the PPZ1 type-1 protein phosphatase. Expression of AtHAL3a in yeast hal3 mutants partially complements their LiCl sensitivity, suggesting possible conserved functions between both proteins. AtHAL3a and AtHAL3b are induced by salt stress and AtHAL3a is the most expressed in non-stressed plants, particularly in seeds. In situ hybridization demonstrates enrichment of AtHAL3a mRNA in seed embryos and in the vascular phloem of different plant tissues. AtHAL3 proteins show striking homology with a group of proteins found in fungi, plants and animals and some homology with a large family of prokaryotic¯avoproteins. Recombinant AtHAL3a protein puri®ed from Escherichia coli was yellow because it contained a non-covalently bound chromophore revealed as¯avin mononucleotide. Transgenic Arabidopsis plants, with gain of AtHAL3a function, show altered growth rates and improved tolerance to salt and osmotic stress.

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Section: Resultsmentioning

confidence: 99%

Arabidopsis thaliana AtHAL3: a flavoprotein related to salt and osmotic tolerance and plant growth

et al. 1999

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“…Amino acid sequence comparison of the predicted RGB1 polypeptide with 55 kDa B regulatory subunits of PP2A polypeptides from other organisms. The aligned sequences are as follows: DmRegB, Drosophila melanogaster (Mayer‐Jaekel et al ., 1993); HsRegB, Homo sapiens (Mayer et al ., 1991); SpRegB, Schizosaccharomyces pombe (Kinoshita et al ., 1996); CtRegB, Candida tropicalis (Rodriguez et al ., 1996); ScRegB, Saccharomyces cerevisiae (Healy et al ., 1991); and AtRegB, Arabidopsis thaliana (Corum et al ., 1996). Gaps (indicated by bars) have been inserted in the amino acid sequences for optimal alignment.…”

Section: Resultsmentioning

confidence: 99%

The B regulatory subunit of protein phosphatase 2A is required for completion of macroconidiation and other developmental processes in Neurospora crassa

Yatzkan

Yarden

1999

Molecular Microbiology

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rgb-1, encoding the tentative B regulatory subunit of the type 2A Ser/Thr phosphatase in Neurospora crassa, was isolated from cDNA and genomic libraries. Based on analysis of cDNA and genomic clones, rgb-1 is 3387 nucleotides in length, contains seven putative introns and encodes a 461-amino-acid polypeptide. Intron I, which is 5' to the presumed translation initiation codon, contains a uORF encoding 34 amino acids. Intron VI undergoes alternative splicing. Inactivation of rgb-1 by the repeat-induced point (RIP) mutation procedure produced progeny that grow slowly, have abnormal hyphal morphology, are female sterile and produce abundant amounts of arthroconidia. The rgb-1RIP strain does not produce major constriction chains or mature macroconidia. Minor constriction chains are formed, yet the growth process reverts to hyphal elongation. Microscopic and genetic analyses indicate that rgb-1 is a regulator of the budding subroutine of the macroconidiation process and that arthroconidiation, which shares common early and late events with macroconidiation, is induced as a default mechanism for asexual reproduction in this fungus.

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“…Osmoregulation mechanisms in C. tropicalis are still largely unknown. It has been shown the role of ion efflux pumps in this process, emphasizing transport systems such as Na + / K + and Na + /H + (Rodriguez et al, 1996; Garcia et al, 1997; Krauke and Sychrova, 2008). Therefore, it is possible that efflux pumps are over expressed by micro-organisms in the coastal environment, in response to the adaptation to stress conditions and may have somehow influenced resistance to some of the antifungal drugs tested by overexpression of efflux pumps.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil

et al. 2016

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Several studies have been developed regarding human health risks associated with the recreational use of beaches contaminated with domestic sewage. These wastes contain various micro-organisms, including Candida tropicalis. In this context, the objective of this study was to characterize C. tropicalis isolates from the sandy beach of Ponta Negra, Natal, Rio Grande do Norte, Brazil, regarding the expression of in vitro virulence factors, adaptation to osmotic stress and susceptibility to antifungal drugs. We analyzed 62 environmental isolates and observed a great variation among them for the various virulence factors evaluated. In general, environmental isolates were more adherent to human buccal epithelial cells (HBEC) than C. tropicalis ATCC13803 reference strain, and they also showed increased biofilm production. Most of the isolates presented wrinkled phenotypes on Spider medium (34 isolates, 54.8%). The majority of the isolates also showed higher proteinase production than control strains, but low phospholipase activity. In addition, 35 isolates (56.4%) had high hemolytic activity (hemolysis index > 0.55). With regard to C. tropicalis resistance to osmotic stress, 85.4% of the isolates were able to grow in a liquid medium containing 15% sodium chloride. The strains were highly resistant to the azoles tested (fluconazole, voriconazole and itraconazole). Fifteen strains were resistant to the three azoles tested (24.2%). Some strains were also resistant to amphotericin B (14 isolates; 22.6%), while all of them were susceptible for the echinocandins tested, except for a single strain of intermediate susceptibility to micafungin. Our results demonstrate that C. tropicalis isolated from the sand can fully express virulence attributes and showed a high persistence capacity on the coastal environment; in addition of showing high minimal inhibitory concentrations to several antifungal drugs used in current clinical practice, demonstrating that environmental isolates may have pathogenic potential.

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CtCdc55p and CtHal3p: Two putative regulatory proteins from Candida tropicalis with long acidic domains

Cited by 14 publications

References 30 publications

Arabidopsis thaliana AtHAL3: a flavoprotein related to salt and osmotic tolerance and plant growth

Arabidopsis thaliana AtHAL3: a flavoprotein related to salt and osmotic tolerance and plant growth

The B regulatory subunit of protein phosphatase 2A is required for completion of macroconidiation and other developmental processes in Neurospora crassa

Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil

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