CT-DNA/BSA Binding Studies of Thiosemicarbazone-Derivated Zn(II) Complex

Abstract

History

Exploring the anticancer potential of thiadiazole derivatives of substituted thiosemicarbazones formed via copper‐mediated cyclization

“…EB's fluorescence intensity is quenched upon displacement because its environment changes from hydrophobic (within DNA strand) to hydrophilic (in aqueous solvent). Water molecules reduce the population of triplet‐state EB by quenching it via abstracting a proton from triplet‐state EB 71–74 . We can observe a minute red shift (2–3 nm) also in the consecutive emission spectra recorded.…”

“…Water molecules reduce the population of triplet-state EB by quenching it via abstracting a proton from triplet-state EB. [71][72][73][74] We can observe a minute red shift (2-3 nm) also in the consecutive emission spectra recorded. The potential intercalative The relative EB displacement capacity of the compounds is proportional to its binding affinity to DNA and can be correlated using the Stern-Volmer/quenching constant, derived from the described equation:…”

“…The intensity of the quenching indicates the efficiency of the compound–protein binding. Synchronous fluorescence spectra of free BSA, and upon gradual addition of CFM and CFP , were observed at constant ∆ λ ( λ emission − λ excitation ) to ascertain the quenching of the intrinsic emission intensity of BSA, which in turn provides a quantitative idea for the binding of the compounds with BSA 71,72 . The quenching of the emission intensities of Trp and Tyr fluorophores of BSA was observed at ∆ λ values of 60 and 15 nm, respectively.…”