CsnA Dependent Development and Regulation of Amino Acid Biosynthesis of the Filamentous Ascomycete <i>Aspergillus nidulans</i>

Abstract

Chapter 1were found: the ones that exhibit light-grown seedling characteristics in the absence of light, a constitutive photomorphogenesis (cop) and the other group of mutants that showed deethiolation (det) . Lethal mutants in this class are allelic to fusca mutants which accumulate anthocyanin, a purple pigment, in the mature seed coat and the embryonic leaves (Gusmaroli et al., 2004;Misera et al., 1994). These mutants were classed as cop/det/fus mutants in A. thaliana and later on it could be shown that their respective gene loci coded for six of the eight subunits of the COP9 signalosome Wei et al., 1998). The mammalian CSN complex is also known as the JAB containing signalosome according to the fifth subunit JAB1 (Carrabino et al., 2004). It was originally the N-terminal HAM domain. The WH-domain is a globular / s t r u c t u r e w i t h a n ""-arrangement, which can be classified as a winged-helix (WH) motif. The HAM domain is entirely helical with a core of six regularly-spaced helices that form three antiparallel helical hairpins. It resembles structurally mainly HEAT and Armadillo-repeats, creating the name HAM-domain. Scheel and Hofmann found that TPR-like (tetratrico-peptide repeats) repeats precede many PCI domains, which consist of short bi-helical segments.