CSN5 Promotes Carcinogenesis of Thyroid Carcinoma Cells Through ANGPTL2

Xie, Peiyi; Wang, Hui; Fang, Jiayu; Du, Dongnian; Tian, Ze; Jing, Zhen; Liu, Yue; Ding, Yiming; Fu, Bidong; Liu, Fanrong; Huang, Da; Yu, Jichun

doi:10.1210/endocr/bqaa206

Cited by 9 publications

(7 citation statements)

References 31 publications

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“…Initially, the LIHC tissue and adjacent tissues were fixed in formalin and embedded in paraffin, and cut into 4-um-thick sections [ 70 ]. After the antigen was repaired by deparaffinization, rehydration, and microwave heating in a microwave-heated antigenic unsealing solution (EDTA, pH 8.0), the slices were sealed with goat serum for 30 minutes.…”

Section: Methodsmentioning

confidence: 99%

ZNF765 is a prognostic biomarker of hepatocellular carcinoma associated with cell cycle, immune infiltration, m6A modification, and drug susceptibility

Ding

Gong

Zeng

et al. 2023

Aging

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Hepatocellular carcinoma (HCC) is an ongoing challenge worldwide. Zinc finger protein 765 (ZNF765) is an important zinc finger protein that is related to the permeability of the blood-tumor barrier. However, the role of ZNF765 in HCC is unclear. This study evaluated the expression of ZNF765 in hepatocellular carcinoma and the impact of its expression on patient prognosis based on The Cancer Genome Atlas (TCGA). Immunohistochemical assays (IHC) were used to examine protein expression. Besides, a colony formation assay was used to examine cell viability. We also explored the relationship between ZNF765 and chemokines in the HCCLM3 cells by qRT-PCR. Moreover, we examined the effect of ZNF765 on cell resistance by measurement of the maximum half-inhibitory concentration. Our research revealed that ZNF765 expression in HCC samples was higher than that in normal samples, whose upregulation was not conducive to the prognosis. The results of GO, KEGG, and GSEA showed that ZNF765 was associated with the cell cycle and immune infiltration. Furthermore, we confirmed that the expression of ZNF765 had a strong connection with the infiltration level of various immune cells, such as B cells, CD4+ T cells, macrophages, and neutrophils. In addition, we found that ZNF765 was associated with m 6 A modification, which may affect the progression of HCC. Finally, drug sensitivity testing found that patients with HCC were sensitive to 20 drugs when they expressed high levels of ZNF765. In conclusion, ZNF765 may be a prognostic biomarker related to cell cycle, immune infiltration, m 6 A modification, and drug sensitivity for hepatocellular carcinoma.

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Section: Methodsmentioning

confidence: 99%

ZNF765 is a prognostic biomarker of hepatocellular carcinoma associated with cell cycle, immune infiltration, m6A modification, and drug susceptibility

Ding

Gong

Zeng

et al. 2023

Aging

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“…The qRT‐PCR was conducted as the previous study 16 . The standard Trizol reagent (Invitrogen, USA) was used to isolate total RNA of cells and tissues.…”

Section: Methodsmentioning

confidence: 99%

“…The qRT‐PCR was conducted as the previous study. 16 The standard Trizol reagent (Invitrogen, USA) was used to isolate total RNA of cells and tissues. The PrimeScript RT Reagent Kit (Invitrogen) was used to reverse transcription, and PCR was conducted by using SYBR Premix Ex Taq (TaKaRa Bio, Japan).…”

Section: Methodsmentioning

confidence: 99%

The deubiquitinase OTUB1 fosters papillary thyroid carcinoma growth through EYA1 stabilization

Xie

Chao

Liu

et al. 2021

J Cellular Molecular Medi

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Deubiquitinating enzyme OTU domain‐containing ubiquitin aldehyde‐binding proteins 1 (OTUB1) has been shown to have an essential role in multiple carcinomas. However, the function of OTUB1 in papillary thyroid cancer (PTC) and the underlying mechanisms regulating PTC cells proliferation remain poorly understood. In this study, OTUB1 was significantly upregulated in papillary thyroid carcinoma tissues and cells. Through in vitro and in vivo experiments, knockdown of OTUB1 suppressed PTC cells growth whereas OTUB1 overexpression enhanced the proliferation ability of PTC cells. Moreover, the eyes absent homologue 1 (EYA1) was recognized as a potential target of OTUB1 through mass spectrometry analysis, and we further verified that EYA1 protein level was positively correlated with OTUB1 expression in PTC cells and clinical samples. Mechanistically, OTUB1 could interact with EYA1 directly and deubiquitinate EYA1 to stabilize it. At last, EYA1 was found to play an essential role in OTUB1‐derived PTC cells growth. Overall, our investigation reveals that OTUB1 is a previously unrecognized oncogenic factor in PTC cells proliferation and suggests that OTUB1 might be a novel therapeutic target in PTC.

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“…JAB1 could also de-ubiquitinate and stabilize PD-L1 which, in turn, leaded to T cell suppression (Lim et al 2016 ). Moreover, JAB1 directly interacted with angiopoietin-like protein 2 (ANGPTL2) and attenuated its ubiquitin-mediated degradation through de-ubiquitylation (Xie et al 2021 ).…”

Section: Molecular Functions Of Jab1mentioning

confidence: 99%

Regulatory mechanisms and therapeutic potential of JAB1 in neurological development and disorders

Song

Gao

et al. 2023

Mol Med

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Abstractc-Jun activation domain binding protein-1 (JAB1) is a multifunctional regulator that plays vital roles in diverse cellular processes. It regulates AP-1 transcriptional activity and also acts as the fifth component of the COP9 signalosome complex. While JAB1 is considered an oncoprotein that triggers tumor development, recent studies have shown that it also functions in neurological development and disorders. In this review, we summarize the general features of the JAB1 gene and protein, and present recent updates on the regulation of JAB1 expression. Moreover, we also highlight the functional roles and regulatory mechanisms of JAB1 in neurodevelopmental processes such as neuronal differentiation, synaptic morphogenesis, myelination, and hair cell development and in the pathogenesis of some neurological disorders such as Alzheimer’s disease, multiple sclerosis, neuropathic pain, and peripheral nerve injury. Furthermore, current challenges and prospects are discussed, including updates on drug development targeting JAB1.

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CSN5 Promotes Carcinogenesis of Thyroid Carcinoma Cells Through ANGPTL2

Cited by 9 publications

References 31 publications

ZNF765 is a prognostic biomarker of hepatocellular carcinoma associated with cell cycle, immune infiltration, m6A modification, and drug susceptibility

ZNF765 is a prognostic biomarker of hepatocellular carcinoma associated with cell cycle, immune infiltration, m6A modification, and drug susceptibility

The deubiquitinase OTUB1 fosters papillary thyroid carcinoma growth through EYA1 stabilization

Regulatory mechanisms and therapeutic potential of JAB1 in neurological development and disorders

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